Rapid identification and differentiation of the vaccine strain Rac H from EHV 1 field isolates using a non-radioactive DNA probe

A method for rapid differentiation between the EHV 1 live vaccine strain Rac H and field isolates is described. Total DNA was isolated from virus-infected small scale cell cultures. DNA fragments digested with restriction endonuclease BamHI were separated, transfered and immobilized on filter membranes. A Digoxigenin-labeled probe derived from EHV 1 was used for hybridization. This probe hybridized specifically to sequences of the inverted terminal repeat region which in case of Rac H include a deletion of 0.8 kb. By comparing the different migration patterns after blot hybridization it could be shown that in 65 isolates from cases of abortion the live vaccine strain Rac H was not involve

Geolectric field measurement, modelling and validation during geomagnetic storms in the UK

Significant geoelectric fields are produced by the interaction of rapidly varying magnetic fields with the conductive Earth, particularly during intense geomagnetic activity. Though usually harmless, large or sustained geoelectric fields can damage grounded infrastructure such as high-voltage transformers and pipelines via geomagnetically induced currents (GICs). A key aspect of understanding the effects of space weather on grounded infrastructure is through the spatial and temporal variation of the geoelectric field. Globally, there are few long-term monitoring sites of the geoelectric field, so in 2012 measurements of the horizontal surface field were started at Lerwick, Eskdalemuir and Hartland observatories in the UK. Between 2012 and 2020, the maximum value of the geoelectric field observed was around 1 V/km in Lerwick, 0.5 V/km in Eskdalemuir and 0.1 V/km in Hartland during the March 2015 storm. These long-term observations also allow comparisons with models of the geoelectric field to be made. We use the measurements to compute magnetotelluric impedance transfer functions at each observatory for periods from 20 to 30,000 s. These are then used to predict the geoelectric field at the observatory sites during selected storm times that match the recorded fields very well (correlation around 0.9). We also compute geoelectric field values from a thin-sheet model of Britain, accounting for the diverse geological and bathymetric island setting. We find the thin-sheet model captures the peak and phase of the band-passed geoelectric field reasonably well, with linear correlation of around 0.4 in general. From these two modelling approaches, we generate geoelectric field values for historic storms (March 1989 and October 2003) and find the estimates of past peak geoelectric fields of up to 1.75 V/km in Eskdalemuir. However, evidence from high voltage transformer GIC measurements during these storms suggests these estimates are likely to represent an underestimate of the true value

Polyaniline/palladium nanohybrids for moisture and hydrogen detection.

Palladium nanoparticles display fascinating electronic, optical and catalytic properties, thus they can be used for various applications such as sensor fabrication. Conducting polymers such as polyaniline have also been widely used in sensor technology due to its cost effectiveness, versatility, and ease of synthesis. In this research, attention was given to unify the exceptional properties of these two materials and construct palladium nanoparticle coated polyaniline films to detect hydrogen and moisture. Electrochemical polymerization of aniline was carried out on gold sputtered epoxy resin boards. Polyaniline film was generated across a gap of 0.2 mm created by a scratch made on the gold coating prior to electrochemical polymerization. A palladium nanoparticle dispersion was prepared using sonochemical reduction method and coated on to polyaniline film using drop-drying technique. Polyaniline only films were also fabricated for comparative analysis. Sensitivity of films towards humidity and hydrogen was evaluated using impedance spectroscopy in the presence of the respective species. According to the results, polyaniline films exhibited an impedance drop in the presence of humidity and the response was significantly improved once palladium nanoparticles were incorporated. Interestingly, polyaniline only films did not respond to hydrogen. Nevertheless, palladium nanoparticle coated polyaniline films exhibited remarkable response towards hydrogen

Crustal 3-D geometry of the Kristineberg area (Sweden) with implications on VMS deposits

Structural analysis of the Palaeoproterozoic volcanogenic massive sulfide (VMS) hosting Kristineberg area, Sweden, constrained by existing magnetotelluric (MT) and seismic reflection data, reveals that the complex geometry characterized by non-cylindrical antiformal structures is due to transpression along the termination of a major high-strain zone. Similar orientations of the host rock deformation fabrics and the VMS ore lenses indicate that the present-day geometry of the complex VMS deposits in the Kristineberg area may be attributed to tectonic transposition. The tectonic transposition was dominantly controlled by reverse shearing and related upright to overturned folding, with increasing contribution of strike-slip shearing and sub-horizontal flow towards greater crustal depths. Furthermore, the northerly dip of the previously recognized subsurface crustal reflector within the Kristineberg area is attributed to formation of crustal compartments with opposite polarities within the scale of the whole Skellefte district. The resulting structural framework of the main geological units is visualized in a 3-D model which is available as a 3-D PDF document through the publication website

An abstract modeling approach towards system-level design-space exploration.

Integration of increasingly complex systems on a chip augments the need of system-level methods for specification and design. In the earliest phases of the design process important design decisions can be taken on the basis of a fast exploration of the design space. This paper describes an abstract modeling approach towards system-level design-space exploration, which is formal and flexible. It uses a uniform system model that contains both functional and architectural information. Disjunct, parameterizable resources represent the real-time behavior of the target architecture. Due to the expressiveness of the modeling language (POOSL), control as well as data oriented behavior can be specified in the functional part of the system model. Well-founded design decisions can be taken as a result of performance estimations that are based on Markov theory

MT and reflection seismics in northwestern Skellefte Ore district, Sweden

A seismic reflection and MT survey was carried out along a 27-km long transect in northwestern Skellefte District, as part of a bigger 3D modeling project. The main motivation for the data acquisition is to elucidate the geologic relationship between the known mineralizations in the Adak mining camp to the north and in the well studied Kristineberg area south of the transect. The seismic reflection data were acquired with a VIBSIST system, and show reflectivity down to 3 s. Apart from the conventional processing for crystalline environments, the seismic data was also subject to an azimuthal binning procedure and crossdip analysis, allowing the orientation of planar reflectors in 3D. Regarding the MT data, it is primarily of good quality along the 17 installed sites. The inversion of the determinant of the impedance tensor yielded a stable 2D resistivity model, dominated by resistors corresponding to the postorogenic intrusions along the transect. Adding the location of the analyzed seismic reflectors in the MT inversion rendered an integrated model that facilitated a preliminary joint interpretation of the data sets. Overall, the results are in good agreement with surface observations and reveal a crude configuration of the geologic units below the transect. The most prominent outcomes are the lateral and depth extent of the large postorogenic intrusions in the area reaching to 5- or 6-km depth, the dimensions of the nearly vertical Brännäs gabbro extending to 6-km depth, and the presence of enhanced conductivities along the transect at about 10 km depth. The latter is probably related to the deep conductor previously identified in the district