Procedure for preparing permeabilized hyphae for enzyme assays

Procedure for preparing permeabilized hyphae for enzyme analysis

Sleep-Dependent Facilitation of Episodic Memory Details

While a role for sleep in declarative memory processing is established, the qualitative nature of this consolidation benefit, and the physiological mechanisms mediating it, remain debated. Here, we investigate the impact of sleep physiology on characteristics of episodic memory using an item- (memory elements) and context- (contextual details associated with those elements) learning paradigm; the latter being especially dependent on the hippocampus. Following back-to-back encoding of two word lists, each associated with a different context, participants were assigned to either a Nap-group, who obtained a 120-min nap, or a No Nap-group. Six hours post-encoding, participants performed a recognition test involving item-memory and context-memory judgments. In contrast to item-memory, which demonstrated no between-group differences, a significant benefit in context-memory developed in the Nap-group, the extent of which correlated both with the amount of stage-2 NREM sleep and frontal fast sleep-spindles. Furthermore, a difference was observed on the basis of word-list order, with the sleep benefit and associated physiological correlations being selective for the second word-list, learned last (most proximal to sleep). These findings suggest that sleep may preferentially benefit contextual (hippocampal-dependent) aspects of memory, supported by sleep-spindle oscillations, and that the temporal order of initial learning differentially determines subsequent offline consolidation

Extraction, purification and analysis of thermal stability of xylose isomerase

Thermostable enzymes are the enzymes which active even at high temperatures, such enzymes are industrially as well as biochemically very important. Xylose isomerase (EC 5.3.1.5) is one such enzyme with suitable commercial applications. It is heat stable and does not require expensive cofactors such as NAD or ATP for activity. The microorganisms producing this enzyme were isolated from hot water spring near ‘Surat’. The organisms were isolated and purified by using different screening methods. The isolated organisms were then subjected to optimum growth conditions for enzyme production. This enzyme was then assayed for its thermal stability at elevated temperatures by using DNSA

Analysis of protease activity of enzyme isolated from compost soil

Bacteria are very good source of enzymes as compared to animal or plant source and even synthetic enzymes. In the present investigation the protease activity has been analyzed. The source of enzyme i.e. protease producers were isolated from compost soil sample viz. collected from the Wanker farm field, Solapur. The protease producers were isolated, screened and grown on a suitable growth medium to obtain maximum production of enzyme. After production the crude enzyme is purified. The purified enzyme is analysed for its keratinolytic activity by using feathers

A comparative clinical study on Tagara-Devadaru Lepa with and without Prachhanna in the management of Indralupta with special reference to Alopecia Areata

Hair disorders causes negative impact towards individual and his/her quality of life. Indralupta is a disease in which hair is lost from some or all areas of the body, usually from scalp. It shows one or more round spot on the scalp . It arises from the vitiation of Tridosha and Rakta. Treatment advised is Siravedhana and topical application of herbal and mineral drugs. To avoid complications of Siravedhana, inthis study Prachhanna was preffered and Tagara-Devadaru chosen as drug for topical application at the site of Indralupta. In this study we had taken 40 Diagnosed patients of Indraluptaand were subjected to clinical trials. They were randomly assigned into two Groups namely Group A and Group B. Group A treated with Tagara-Devadaru Lepa, while subject under Group B treated by Prachhanna along with Tagara-Devadaru Lepa. The treatment modalities of TagaraDevadaru Lepa and Prachhanna with Tagara-Devadaru Lepa are equally efficacious in treating.On comparing the results of Group A and Group B, the conclusion were drawn.Both the methods of treatment are cost effective, easy to prepare and had no adverse effects

A clinical comparative study of Chedana Paschat Arka Pratisaraeeiya Kshara & Agnikarma in the management of Kadara (Corn)

Sushruta Samhita is the earliest known authentic treatise on Ayurveda. In Vedic period also there is a description of Agnikarma and Ksharakarma. In Shalya Tantra common procedure are Agnikarma, Ksharakarma and Jalokavacharna etc. Kadara is one among Kshudra Roga is claimed to be effectively dealt with Agnikarma and Ksharakarma. This is intended to offer instant relief to the patient to evolve a simple and economic management and to evaluate whether the relapse can be prevented with Agnikarma and Ksharakarma.The studies conducted with 40 patients were selected, made into 2 groups of 20 each patients. Group A patients were treated with surgical excision followed by Arka Pratisaraneeya Ksharakarma and Group B patients were treated with surgical excision followed by Agnikarma. Arka Kshara is prepared and applied after excision of lesion, keeping for 100 Shatamatrakala (100 sec), treated with Nimbuka Swarasa. With all aseptic measures the lesion is excised and then Agnikarma using Lohashalaka, Madhu and Sarpi is applied and bandaged. Both procedures are in single sitting, dressing for alternate day and every 15 days follow up till 45 days. After the completion of clinical trial, it was found that Agnikarma procedure there was highly significant results in reducing pain, discomfort, bleeding infections and healing period. Where as in KsharaKarma there is also significant result in reducing pain, discomfort but statically considering average mean Kshara Karma shows comparatively lesser effective than Agnikarma. By the statistical results it can be concluded that Agnikarma has better result when compared to Kshara Karma in the present study

Compact Layers of Hybrid Halide Perovskites Fabricated via the Aerosol Deposition Process : Uncoupling Material Synthesis and Layer Formation

We present the successful fabrication of CH3NH3PbI3 perovskite layers by the aerosol deposition method (ADM). The layers show high structural purity and compactness, thus making them suitable for application in perovskite-based optoelectronic devices. By using the aerosol deposition method we are able to decouple material synthesis from layer processing. Our results therefore allow for enhanced and easy control over the fabrication of perovskite-based devices, further paving the way for their commercialization