3,347 research outputs found
Murine terminal deoxynucleotidyl transferase: cellular distribution and response to cortisone
The mouse thymus contains two forms of terminal deoxynucleotidyl transferase (TdT) which are distinguishable by the salt concentration necessary to elute them from a phosphocellulose column, by their distrubtion among the thymocyte subpopulations, and by their sensitivity to cortisone treatment. In the whole thymus the later eluting peak (peak II) is the predominant one with about 3-10% of the total activity appearing in peak I. Both peak I and peak II activities are most sensitively assayed by the polymerization of dGMP onto an oligo(dA) primer. The minor population of thymocytes which is less dense and cortisone-resistant contains a higher specific activity of peak I TdT. The majority of TdT activity is, however, found in the major population of thymocytes which occurs in the center region of a bovine serum albumin gradient and is cortisone-sensitive. A very low level of an activity indistinguishable from peak II TdT activity is also detected in the mouse bone marrow. Other tissues, such as spleen, liver, heart, and brain lack detectable amounts of TdT activity
Doping Evolution of Oxygen K-edge X-ray Absorption Spectra in Cuprate Superconductors
We study oxygen K-edge x-ray absorption spectroscopy (XAS) and investigate
the validity of the Zhang-Rice singlet (ZRS) picture in overdoped cuprate
superconductors. Using large-scale exact diagonalization of the three-orbital
Hubbard model, we observe the effect of strong correlations manifesting in a
dynamical spectral weight transfer from the upper Hubbard band to the ZRS band.
The quantitative agreement between theory and experiment highlights an
additional spectral weight reshuffling due to core-hole interaction. Our
results confirm the important correlated nature of the cuprates and elucidate
the changing orbital character of the low-energy quasi-particles, but also
demonstrate the continued relevance of the ZRS even in the overdoped region.Comment: Original: 5 pages, 4 figures. Replaced: 6 pages and 4 figures, with
updated title and conten
Doping evolution of spin and charge excitations in the Hubbard model
To shed light on how electronic correlations vary across the phase diagram of
the cuprate superconductors, we examine the doping evolution of spin and charge
excitations in the single-band Hubbard model using determinant quantum Monte
Carlo (DQMC). In the single-particle response, we observe that the effects of
correlations weaken rapidly with doping, such that one may expect the random
phase approximation (RPA) to provide an adequate description of the
two-particle response. In contrast, when compared to RPA, we find that
significant residual correlations in the two-particle excitations persist up to
hole and electron doping (the range of dopings achieved in the
cuprates). These fundamental differences between the doping evolution of
single- and multi-particle renormalizations show that conclusions drawn from
single-particle processes cannot necessarily be applied to multi-particle
excitations. Eventually, the system smoothly transitions via a
momentum-dependent crossover into a weakly correlated metallic state where the
spin and charge excitation spectra exhibit similar behavior and where RPA
provides an adequate description.Comment: 5 pages, 4 figures, plus supplementary materia
Ion Pairs for Transdermal and Dermal Drug Delivery: A Review
Ion pairing is a strategy used to increase the permeation of topically applied ionised drugs. Formation occurs when the electrostatic energy of attraction between oppositely charged ions exceeds their mean thermal energy, making it possible for them to draw together and attain a critical distance. These ions then behave as a neutral species, allowing them to partition more readily into a lipid environment. Partition coefficient studies may be used to determine the potential of ions to pair and partition into an organic phase but cannot be relied upon to predict flux. Early researchers indicated that temperature, size of ions and dielectric constant of the solvent system all contributed to the formation of ion pairs. While size is important, this may be outweighed by improved lipophilicity of the counter ion due to increased length of the carbon chain. Organic counter ions are more effective than inorganic moieties in forming ion pairs. In addition to being used to increase permeation, ion pairs have been used to control and even prevent permeation of the active ingredient. They have also been used to stabilise solid lipid nanoparticle formulations. Ion pairs have been used in conjunction with permeation enhancers, and permeation enhancers have been used as counter ions in ion pairing. This review attempts to show the various ways in which ion pairs have been used in drug delivery via the skin. It also endeavours to extract and consolidate common approaches in order to inform future formulations for topical and transdermal delivery
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