Observations on the distribution of plankton at six inshore stations in the Gulf of Manaar

It has long been recognised that the distribution of plankton may be very patchy, especially in the coastal regions because near the land the sea may be frequently disturbed over small areas by the mixing of coastal and oceanic waters, tidal streams and Ibe upwelling of the lower layers of water against ceastal banks. This is further complicated by the sporadic outbursts of larva I forms from the littoral fauna and the shallow water benthos

Energy Efficient UAV-Assisted Emergency Communication with Reliable Connectivity and Collision Avoidance

Emergency communication is vital for search and rescue operations following natural disasters. Unmanned Aerial Vehicles (UAVs) can significantly assist emergency communication by agile positioning, maintaining connectivity during rapid motion, and relaying critical disaster-related information to Ground Control Stations (GCS). Designing effective routing protocols for relaying crucial data in UAV networks is challenging due to dynamic topology, rapid mobility, and limited UAV resources. This paper presents a novel energy-constrained routing mechanism that ensures connectivity, inter-UAV collision avoidance, and network restoration post-UAV fragmentation while adapting without a predefined UAV path. The proposed method employs improved Q learning to optimize the next-hop node selection. Considering these factors, the paper proposes a novel, Improved Q-learning-based Multi-hop Routing (IQMR) protocol. Simulation results validate IQMRs adaptability to changing system conditions and superiority over QMR, QTAR, and QFANET in energy efficiency and data throughput. IQMR achieves energy consumption efficiency improvements of 32.27%, 36.35%, and 36.35% over QMR, Q-FANET, and QTAR, along with significantly higher data throughput enhancements of 53.3%, 80.35%, and 93.36% over Q-FANET, QMR, and QTAR.Comment: 13 page

Delayed interval twin delivery of a fetus with a favourable neonatal outcome after a preterm delivery of the first twin: a case report

Assisted reproductive techniques have proved to be a boon for infertile couples. With advent of newer techniques, the incidence of successful multiple pregnancies has also risen. Considering the emotional and financial aspects of the treatment and the risk of preterm delivery in such cases, our intent is not only to salvage one of the twins in case of unfortunate preterm delivery of the other but also to deliver a viable second twin with better chance of survival and favourable neonatal outcome. The current case describes a 34-year woman with previous 2 failed IVF conceptions, on external progesterone support, carrying a twin gestation in preterm labour. Upon the inadvertent delivery of the first twin, a cervical cerclage was done, and she was given conservative management, including bed rest and head low position in view of short cervix, with an aim to delay the delivery of the other. An interval of 66 days was achieved with surgical as well as medical management, following which a healthy second twin was born

Hierarchical amino acid utilization and its influence on fermentation dynamics: rifamycin B fermentation using Amycolatopsis mediterranei S699, a case study

BACKGROUND: Industrial fermentation typically uses complex nitrogen substrates which consist of mixture of amino acids. The uptake of amino acids is known to be mediated by several amino acid transporters with certain preferences. However, models to predict this preferential uptake are not available. We present the stoichiometry for the utilization of amino acids as a sole carbon and nitrogen substrate or along with glucose as an additional carbon source. In the former case, the excess nitrogen provided by the amino acids is excreted by the organism in the form of ammonia. We have developed a cybernetic model to predict the sequence and kinetics of uptake of amino acids. The model is based on the assumption that the growth on a specific substrate is dependent on key enzyme(s) responsible for the uptake and assimilation of the substrates. These enzymes may be regulated by mechanisms of nitrogen catabolite repression. The model hypothesizes that the organism is an optimal strategist and invests resources for the uptake of a substrate that are proportional to the returns. RESULTS: Stoichiometric coefficients and kinetic parameters of the model were estimated experimentally for Amycolatopsis mediterranei S699, a rifamycin B overproducer. The model was then used to predict the uptake kinetics in a medium containing cas amino acids. In contrast to the other amino acids, the uptake of proline was not affected by the carbon or nitrogen catabolite repression in this strain. The model accurately predicted simultaneous uptake of amino acids at low cas concentrations and sequential uptake at high cas concentrations. The simulated profile of the key enzymes implies the presence of specific transporters for small groups of amino acids. CONCLUSION: The work demonstrates utility of the cybernetic model in predicting the sequence and kinetics of amino acid uptake in a case study involving Amycolatopsis mediterranei, an industrially important organism. This work also throws some light on amino acid transporters and their regulation in A. mediterranei .Further, cybernetic model based experimental strategy unravels formation and utilization of ammonia as well as its inhibitory role during amino acid uptake. Our results have implications for model based optimization and monitoring of other industrial fermentation processes involving complex nitrogen substrate

Development of a multivariable risk model integrating urinary cell DNA methylation and cell-free RNA data for the detection of significant prostate cancer

Background: Prostate cancer exhibits severe clinical heterogeneity and there is a critical need for clinically implementable tools able to precisely and noninvasively identify patients that can either be safely removed from treatment pathways or those requiring further follow up. Our objectives were to develop a multivariable risk prediction model through the integration of clinical, urine-derived cell-free messenger RNA (cf-RNA) and urine cell DNA methylation data capable of noninvasively detecting significant prostate cancer in biopsy naïve patients. Methods: Post-digital rectal examination urine samples previously analyzed separately for both cellular methylation and cf-RNA expression within the Movember GAP1 urine biomarker cohort were selected for a fully integrated analysis (n = 207). A robust feature selection framework, based on bootstrap resampling and permutation, was utilized to find the optimal combination of clinical and urinary markers in a random forest model, deemed ExoMeth. Out-of-bag predictions from ExoMeth were used for diagnostic evaluation in men with a clinical suspicion of prostate cancer (PSA ≥ 4 ng/mL, adverse digital rectal examination, age, or lower urinary tract symptoms). Results: As ExoMeth risk score (range, 0-1) increased, the likelihood of high-grade disease being detected on biopsy was significantly greater (odds ratio = 2.04 per 0.1 ExoMeth increase, 95% confidence interval [CI]: 1.78-2.35). On an initial TRUS biopsy, ExoMeth accurately predicted the presence of Gleason score ≥3 + 4, area under the receiver-operator characteristic curve (AUC) = 0.89 (95% CI: 0.84-0.93) and was additionally capable of detecting any cancer on biopsy, AUC = 0.91 (95% CI: 0.87-0.95). Application of ExoMeth provided a net benefit over current standards of care and has the potential to reduce unnecessary biopsies by 66% when a risk threshold of 0.25 is accepted. Conclusion: Integration of urinary biomarkers across multiple assay methods has greater diagnostic ability than either method in isolation, providing superior predictive ability of biopsy outcomes. ExoMeth represents a more holistic view of urinary biomarkers and has the potential to result in substantial changes to how patients suspected of harboring prostate cancer are diagnosed