In vitro and in vivo targeting of different folate receptor-positive cancer cell lines with a novel 99mTc-radiofolate tracer

ISSN:1619-7070ISSN:1619-708

Can animal data predict human outcome?: Problems and pitfalls of translational animal research

ISSN:1619-7070ISSN:1619-708

Preclinical evaluation of novel organometallic 99mTc-folate and 99mTc-pteroate radiotracers for folate receptor-positive tumour targeting

ISSN:1619-7070ISSN:1619-708

A stable neurotensin-based radiopharmaceutical for targeted imaging and therapy of neurotensin receptor-positive tumors

ISSN:1619-7070ISSN:1619-708

Impact of inherent variability and experimental parameters on the reliability of small animal PET data

Background Noninvasive preclinical imaging methodologies such as small animal positron emission tomography (PET) allow the repeated measurement of the same subject which is generally assumed to reduce the variability of the experimental outcome parameter and to produce more robust results. In this study, the variability of tracer uptake in the rodent brain was assessed within and between subjects using the established radiopharmaceuticals 18F-FDG and 18F-fallypride. Moreover, experimental factors with potential impact on study outcome were elicited, and the effect of their strict homogenization was assessed. Methods Brain standardized uptake values of rodents were compared between three PET scans of the same animal and scans of different individuals. 18F-FDG ex vivo tissue sampling was performed under variation of the following experimental parameters: gender, age, cage occupancy, anesthetic protocol, environmental temperature during uptake phase, and tracer formulation. Results No significant difference of variability in 18F-FDG or 18F-fallypride brain or striatal uptake was identified between scans of the same and scans of different animals (COV = 14 ± 7% vs. 21 ± 10% for 18F-FDG). 18F-FDG brain uptake was robust regarding a variety of experimental parameters; only anesthetic protocols showed a significant impact. In contrast to a heterogenization approach, homogenization of groups produced more false positive effects in 18F-FDG organ distribution showing a false positive rate of 9% vs. 6%. Conclusions Repeated measurements of the same animal may not reduce data variability compared with measurements on different animals. Controlled heterogenization of test groups with regard to experimental parameters is advisable as it decreases the generation of false positive results and thus increases external validity of study outcome.ISSN:2191-219

Functional Brain Receptor Imaging with Positron Emission Tomography

A new cocaine derivative for imaging the dopamine transporter has been developed. Measurements of radioligand binding of 11C-(+)-McN-5652 in vivo with PET suggests that ecstasy interacts directly with the serotonin reuptake sites and that a single oral dose of ecstasy (1.5 mg/kg) does not cause any changes in the serotonin transporter density in the human brain. Finally, a number of epibatidine derivatives have been developed as ligands to study the central nAChRs in vivo, however, toxicity studies prevented further clinical use

Imaging of renal carcinoma xenografts with Cu-64-labelled anti-L1-CAM antibody chCE7

ISSN:1619-7070ISSN:1619-708