151 research outputs found
Adaptation of Small Mammals to Conditions of Biogeochemical Province with the Increased Level of Heavy Metals
Abstract-At present, the natural non-endemic geochemical anomalies impacts on biota are not studied enough. The purpose of this work was to study effects of the natural geochemical anomaly with high content of nickel, cobalt and chrome on animals. The anomalous environment accounts for low abundance of small mammals, changes in the interior weight characteristics, alterations in the adrenal cortex structure, and reproductive function modifications. Natural anomalies serve as a factor of the additional functional load upon reproductive and endocrine systems
Design of catalysts based on silver nanoparticles and diatomite for photocatalytic decomposition of phenol
Lambda-inflation and CMB anisotropy
We explore a broad class of three-parameter inflationary models, called the
-inflation, and its observational predictions: high abundance of
cosmic gravitational waves consistent with the Harrison-Zel'dovich spectrum of
primordial cosmological perturbations, the non-power-law wing-like spectrum of
matter density perturbations, high efficiency of these models to meet current
observational tests, and others. We show that a parity contribution of the
gravitational waves and adiabatic density perturbations into the large-scale
temperature anisotropy, T/S , is a common feature of
-inflation; the maximum values of T/S (basically not larger than 10)
are reached in models where (i) the local spectrum shape of density
perturbations is flat or slightly red (), and (ii) the residual
potential energy of the inflaton is near the GUT scale (). The conditions to find large T/S in the paradigm of cosmic
inflation and the relationship of T/S to the ratio of the power spectra, ,
and to the inflationary and Hubble parameters, are discussed. We argue
that a simple estimate, T/S, is true for most known inflationary solutions and allows
to relate straightforwardly the important parameters of observational and
physical cosmology.Comment: 29 pages, 3 figures include
Review of scientific topics for Millimetron space observatory
This paper describes outstanding issues in astrophysics and cosmology that
can be solved by astronomical observations in a broad spectral range from far
infrared to millimeter wavelengths. The discussed problems related to the
formation of stars and planets, galaxies and the interstellar medium, studies
of black holes and the development of the cosmological model can be addressed
by the planned space observatory Millimetron (the "Spectr-M" project) equipped
with a cooled 10-m mirror. Millimetron can operate both as a single-dish
telescope and as a part of a space-ground interferometer with very long
baseline.Comment: The translation of the original article in Physics Uspekhi
http://ufn.ru/ru/articles/2014/12/c
The interaction of dark matter cusp with the baryon component in disk galaxies
In this paper we examine the effect of the formation and evolution of the
disk galaxy on the distribution of dark halo matter. We have made simulations
of isolated dark matter (DM) halo and two component (DM + baryons). N-body
technique was used for stellar and DM particles and TVD MUSCL scheme for
gas-dynamic simulations. The simulations include the processes of star
formation, stellar feedback, heating and cooling of the interstellar medium.
The results of numerical experiments with high spatial resolution let us to
conclude in two main findings. First, accounting of star formation and
supernova feedback resolves the so-called problem of cusp in distribution of
dark matter predicted by cosmological simulations. Second, the interaction of
dark matter with dynamic substructures of stellar and gaseous galactic disk
(e.g., spiral waves, bar) has an impact on the shape of the dark halo. In
particular, the in-plane distribution of dark matter is more symmetric in runs,
where the baryonic component was taken into account.Comment: 7 pages, 6 figure
Observational Manifestations of the First Protogalaxies in the 21 cm Line
The absorption properties of the first low-mass protogalaxies (mini-halos)
forming at high redshifts in the 21-cm line of atomic hydrogen are considered.
The absorption properties of these protogalaxies are shown to depend strongly
on both their mass and evolutionary status. The optical depths in the line
reach 0.1-0.2 for small impact parameters of the line of sight. When a
protogalaxy being compressed, the influence of gas accretion can be seen
manifested in a non-monotonic frequency dependence of the optical depth. The
absorption characteristics in the 21-cm line are determined by the thermal and
dynamical evolution of the gas in protogalaxies. Since the theoretical line
width in the observer's reference frame is 1-6 kHz and the expected separation
between lines 8.4 kHz, the lines from low mass protogalaxies can be resolved
using ongoing and future low frequency interferometers.Comment: 12 pages, 5 figure
Hydrogen storage in LiAlH4: Predictions of the crystal structures and reaction mechanisms of intermediate phases from quantum mechanics
Production of Bst polymerase for diagnosis of different infections using loop-mediated isothermal amplification
Introduction. The large fragment of DNA polymerase I from Geobacillus stearothermophilus GIM1.543 (Bst DNA polymerase) possesses 5'-3' DNA polymerase activity, 5'-3' displacement activity and high processivity. These properties make it possible to use Bst DNA polymerase in loop-mediated isothermal amplification (LAMP), which provides highly specific amplification of the target sequence and is used for rapid detection of agents causing human infectious diseases.
The purpose of the study was to produce a recombinant Bst polymerase enzyme in the bacterial expression system and to assess its properties for LAMP-based diagnostics of infectious diseases.
Materials and methods. Expression constructs carrying the Bst polymerase gene were obtained using genetic engineering techniques. Different Escherichia coli strains were used for protein expression. Metal-chelate and gel filtration chromatography techniques were used for protein purification. Catalytic characteristics of the enzyme were assessed in loop-mediated isothermal amplification reactions using AmpliSens SARS-CoV-2-IT, AmpliSens IAV-IT and AmpliSens IBV-IT diagnostic systems designed for high-quality detection of SARS-CoV-2, influenza A virus (IAV) and influenza B virus (IBV) RNA, respectively.
Results. The offered protocol for production, extraction and purification of recombinant Bst polymerase makes it possible to produce the enzyme in the bacterial expression system using E. coli cells in a soluble form and reaching the yield up to 20% of the total cell mass. In LAMP reactions, the obtained enzyme demonstrates activity comparable with that of the commercial enzyme Bst 2.0 (NEB).
Conclusion. Considering the fast purification and production of the enzyme, the obtained recombinant Bst polymerase can be used in LAMP-based diagnostic kits
IDENTIFICATION AND STUDY OF LEPTOSPIROSIS FOCUS IN IRKUTSK REGION
Due to examination of animal breeding workers at the Irkutsk Region a case of human leptospirosis was identified. at the cattle farm of Tugutuy settlement. Due to epizootological-epidemiological study a mixed, leptospirosis focus in Ekhirit-Bulagat district was established, the agent circulation among commensal and wild, small mammals was demonstrated. Continuous pathogenic Leptospira DNA detection during the second, half of 2011, as well as the positive tularemia laboratory results require further study of the focus
God “Will Repay Each One According to His Deeds”: From the History of the Orthodox Worldview (on Materials from Old Believers Literature)
В монографии рассказывается об основных аспектах традиционного русского менталитета, отраженных в памятниках письменности русского старообрядчества. В этой литературе, разнообразной по жанрам и аспектам темы, зафиксированы отношение старообрядцев к власти и их интерпретация исторических событий, отражены душевные переживания, рассуждения о судьбах человечества в целом и отдельного человека. Основное внимание в книге сосредоточено на эсхатологических теориях, занимающих особое место в старообрядческой книжной культуре. Для широкого круга читателей, интересующихся проблемами российской истории и культуры.The monograph tells about the main aspects of the traditional Russian mentality, reflected in the literary artefacts of the Russian Old Believers. This literature, diverse in genres and problems, conveys the authors’ attitude to authority, their interpretation of historical events, emotional experiences, reasoning on the fate of individual people and humanity as a whole. The main attention in the book is focused on eschatological theories that occupy a special place in the Old Believer bookish culture. The book is aimed at a wide range of readers interested in Russian history and culture.Монография написана коллективом Лаборатории археографических исследований в рамках выполнения госзадания Министерства науки и образования по теме «Региональная идентичность России: компаративные историко-филологические исследования» № FEUZ-2020-0056. Книга издана при поддержке Эндаумент-фонда Уральского федерального университета и целевого капитала «Истфак — Чемпион!
- …