Micropropagation of a Valuable Ethnomedicinal Plant Streblus asper Lour.

A micropropagation protocol is presented for conservation of critically threatened woody tree species, Streblus asper Lour. In vitro axillary bud proliferation followed by multiple shoot induction was obtained using mature nodal segments. Initially, explants were cultured on Murashige and Skoog’s medium supplemented with different concentrations of BA (2.2, 4.4, 6.6, 8.9, 11.1 and 13.3 µM), Kn (2.3, 4.6, 6.9, 9.3, 11.6 and 13.9 µM) or TDZ (2.2, 4.5, 6.7, 8.90, 11.1 and 13.5 µM). These individual levels of cytokinins did not support in vitro shoot regeneration in S. asper. Combinations of cytokinins, Kn with BA or TDZ, significantly influenced shoot regeneration ability. The combination of Kn (4.60 µM) with BA (4.44 µM) evoked an optimum response towards shoot proliferation whereas, medium containing Kn (4.60 µM) plus TDZ (4.54 µM) induced multiple shoot formation. In vitro developed microshoots were rooted on MS half strength medium supplemented with 2.46 µM IBA. The plantlets established in vitro were transferred to pots containing sterilized soil and vermiculite (1:1) mixture and were hardened in the greenhouse with 70-75% survival rate. Key words: Clonal propagation, Cytokinins, Mature nodal explant, Medicinal woody tree, Multiple shoot induction Abbreviations: BA - 6-benzyladenine, IBA - indole 3-butyric acid, Kn - kinetin, µM - micro moles, MS – Murashige and Skoog TDZ - thidiazuron Kranthi Gadidasu et al. Micropropagation of a Valuable Ethnomedicinal Plant Streblus asper Lour.. J Phytol 3/2 (2011) 18-2

Mulberry improvements via plastid transformation and tissue culture engineering

The in vitro tissue culture and micropropagation studies for Morus spp., a pivotal sericulture plant, are well established. The rapid and reproducible in vitro response to plant growth regulator treatments has emerged as an essential complement of transformation studies for this plant species. A major area of study is the use of protoplast culture and fusion techniques where advantages to mulberry improvement can be applied. The advancements in genetic transformation of mulberry are reviewed, and a section on strategy for transforming plastids (chloroplasts) of mulberry is included. A role for mulberry in “molecular farming” is envisioned. The conclusions and future prospects for improvement of this economically important tree species are proposed

Comparative Study of Rfid Tags for Metallic Products in Inventory Tracking System

It has always been challenging and critical to implement Radio Frequency Identification tags and technologies for inventory tracking for metallic product because of interference of metal on RFID signals, rough environment especially in presence of extreme moisture  and high temperature  in manufacturing units. A very extensive research and experiments are needed, to choose Radio Frequency Identification tags for metallic products in manufacturing. Therefore by comparing various RFID technologies, its features and performance we will try to find out availability and feasibility of Radio Frequency Identification Tags which are most suitable, cost effective and efficient for Metallic Products especially in harsh manufacturing environment.This paper presents a comparative study where we will assess and compare various features and performance parameters of Radio Frequency Identification tags available in the world and review their technology, implementation, working efficiency and cost effectiveness in depth so as to get a clear understanding about tag's performance and feasibility on Metallic product. It will help in taking better decision in the selection of Radio Frequency Identification Tags for Metallic products for inventory tracking system

Construction, database integration, and application of an Oenothera EST library

AbstractCoevolution of cellular genetic compartments is a fundamental aspect in eukaryotic genome evolution that becomes apparent in serious developmental disturbances after interspecific organelle exchanges. The genus Oenothera represents a unique, at present the only available, resource to study the role of the compartmentalized plant genome in diversification of populations and speciation processes. An integrated approach involving cDNA cloning, EST sequencing, and bioinformatic data mining was chosen using Oenothera elata with the genetic constitution nuclear genome AA with plastome type I. The Gene Ontology system grouped 1621 unique gene products into 17 different functional categories. Application of arrays generated from a selected fraction of ESTs revealed significantly differing expression profiles among closely related Oenothera species possessing the potential to generate fertile and incompatible plastid/nuclear hybrids (hybrid bleaching). Furthermore, the EST library provides a valuable source of PCR-based polymorphic molecular markers that are instrumental for genotyping and molecular mapping approaches