Standardized next-generation sequencing of immunoglobulin and T-cell receptor gene recombinations for MRD marker identification in acute lymphoblastic leukaemia; a EuroClonality-NGS validation study

Amplicon-based next-generation sequencing (NGS) of immunoglobulin (IG) and T-cell receptor (TR) gene rearrangements for clonality assessment, marker identification and quantification of minimal residual disease (MRD) in lymphoid neoplasms has been the focus of intense research, development and application. However, standardization and validation in a scientifically controlled multicentre setting is still lacking. Therefore, IG/TR assay development and design, including bioinformatics, was performed within the EuroClonality-NGS working group and validated for MRD marker identification in acute lymphoblastic leukaemia (ALL). Five EuroMRD ALL reference laboratories performed IG/TR NGS in 50 diagnostic ALL samples, and compared results with those generated through routine IG/TR Sanger sequencing. A central polytarget quality control (cPT-QC) was used to monitor primer performance, and a central in-tube quality control (cIT-QC) wa

Torsional deformations in incompressible fibre–reinforced cylindrical pipes

The first part of the paper deals with an extension of the classical Rivlin’s solution of the torsion problem of a neo-Hookean pipe. The second part concerns a study of the passive torsional deformation processes in a fibre-reinforced cylindrical dummy of the beating heart. Especially, the dependence of the torsional and volumetric stiffness of the cylindrical pipe on different geometric and material parameters is discussed through a set of numerical simulations

Passive and Active Deformation Processes in Cardiac Tissue

We present a continuum model able to describe the mechanism of muscle contractions from a macroscopic point of view

Distributed HPC Resources Orchestration for Supporting Large-Scale Workflow Execution

Scientific and industrial applications have become more complex over the past decades and nowadays include multi-domain workflows (e.g. ingestion of large (unstructured) datasets, high-performance computing simulations, machine learning tasks). This chapter discusses the EU-funded H2020 LEXIS project approach to the effective management of such multi-domain workflows and their execution on heterogeneous and geographically distributed computing resources. The LEXIS project solution aims at providing users with a tool to describe all the steps involved in the execution of the application (i.e. the workflow) and their execution on the most appropriate pool of resources