Strongly perturbed harmonic oscillator

The limits of current micro-scale technology is approaching rapidly. As the technology is going toward nano-scale devices, physical phenomena involved are fundamentally different from micro-scale ones [1], [2]. Principles in classical physics are no longer powerful enough to explicate the phenomena involved in nano-scale devices. At this stage, quantum mechanic sheds some light on those topics which cannot be described by classical physics. The primary focus of this research work is the development of an analysis technique for understanding the behavior of strongly perturbed harmonic oscillators. Developing ``auxiliary'' boundary value problems we solve monomially perturbed harmonic oscillators. Thereby, we assume monomial terms of arbitrary degree and any finite coefficient desired. The corresponding eigenvalues and eigenvectors can be utilized to solve more complex anharmonic oscillators with non polynomial anharmonicity or numerically defined anharmonicity. A large number of numerical calculations demonstrate the robustness and feasibility of our technique. Particular attention has been paid to the details as have implemented the underlying formula. We have developed iterative expressions for the involved integrals and the introduced ``Universal Functions.'' The latter are applications and adaptations of a concept which was developed in 1990's to accelerate computations in the Boundary Element Method

Effects of low intensity light therapy on cancer cells : in vitro evaluation

Low intensity light therapy (LILT) is an emerging non-invasive modality for localized treatment of joint pain, bone and soft tissue repair and other medical conditions. It is proven that changes in the energy state of bio-molecules induced by electromagnetic radiation (EMR) lead to changes in biological functions of irradiated bio-molecules. By using the Resonant Recognition Model (RRM) approach, it was computationally predicted that far infrared light irradiation in the range of 3500nm – 6000nm affects biological activity of proto-oncogene proteins. This project developed the exposure system and evaluated the effect of external visible, near infrared and far infrared wavelengths in vitro on selected human (MCF7, human breast cancer cells, and HEM, human normal cells) and animal (B16F10, animal cancer cells, and CHO, animal normal cells) cells for three different exposure regimes. Extensive cell based quantitative and qualitative assays conducted on irradiated cells to evaluate evaluation the effect of external light radiation on cells. Quantitative assessments were performed by LDH, MTT, and PrestoBlue. Qualitative assessments were achieved by Confocal Laser Scanning Microscopy (CLSM) and phase contrast microscopy. These in vitro experiments of selected cancer and normal cells demonstrate that the theoretically proposed wavelengths induce cytotoxic effects in cancer cells for all three regimes of exposures and post exposure incubation, while such effect could not be verified for normal cells

Influence of far infrared radiation on cytotoxicity of human breast cancer (MCF7) cells: experimental evaluation

The fact that low intensity light has certain therapeutic effect has been proven through a number of research studies. There are studies that showed the applied electromagnetic radiation (EMR) in the visible and infrared light range can modulate protein and cellular activity. Here we have evaluated experimentally the hypothesis of the Resonant Recognition Model (RRM) that selectivity of protein activities is based on specific resonant electromagnetic interactions. The RRM theory proposes that an external electromagnetic field at a particular activation frequency would produce resonant effects on a protein biological activity, and this activation frequency can be determined computationally [1]. In our previous study 46 oncogene proteins were analyzed using the RRM and their characteristic frequency that correspond to their common biological activity was determined [2]. As reported in [3], the wavelengths of the applied electromagnetic radiation (EMR) in a range of 3500-6400nm are expected to affect biological activity of oncogene proteins. Thus, we designed the exposure system based on IR-LED to irradiate the selected cancer and normal cells in the wavelength range predicted computationally by the RRM [3]. The experimental evaluation of the attained far infrared wavelengths of 3400nm, 3600nm, 3800nm, 3900nm, 4100nm and 4300nm was conducted in vitro on Human Breast Cancer (MCF7) and Human Epidermal Melanocytes, which were used in this study as a control

Low intensity light therapy exposure system

Low intensity light (LIL) has proven to have effects on biological processes in human body, so it can be used for certain medical applications. Many of biological processes are light inducing and frequency selective processes which relate to quantum energy state of photosensitive molecules. There is considerable evidence that induced changes of energy state in bio-molecules would lead to some biological processes in cells. The resonant recognition model (RRM) was employed to determine the frequency/wavelength of the applied electromagnetic radiation (3500-6400nm) that can affect biological activity of oncogene proteins. The RRM is designed for analysis of protein-protein and protein-DNA interaction

In vitro evaluation of low-intensity light radiation on murine melanoma (B16F10) cells

Changes in the energy state of biomolecules induced by electromagnetic radiation lead to changes in biological functions of irradiated biomolecules.Using the RRM approach, it was computationally predicted that far-infrared light irradiation in the range of 3500-6000 nm affects biological activity of proto-oncogene proteins. This in vitro study evaluates quantitatively and qualitatively the effects of selected far-infrared exposures in the computationally determined wavelengths on mouse melanoma B16F10 cells and Chinese hamster ovarian (CHO) cells by MTT (thiazolyl blue tetrazolium bromide) cell proliferation assay and confocal laser-scanning microscopy (CLSM). This paper also presents the findings obtained from irradiating B16F10 and CHO cells by the selected wavelengths in visible and near-infrared range. The MTT results show that far-infrared wavelength irradiation induces detrimental effect on cellular viability of B16F10 cells, while that of normal CHO cells is not affected considerably. Moreover, CLSM images demonstrate visible cellular detachment of cancer cells. The observed effects support the hypothesis that far-infrared light irradiation within the computationally determined wavelength range induces biological effect on cancer cells. From irradiation of selected visible and near-infrared wavelengths, no visible changes were detected in cellular viability of either normal or cancer cells

In vitro evaluation of visible, near - and far infrared light radiation on cancer and normal cells

There is strong evidence that the changes in energy states of bio-molecules, induced by applied electromagnetic radiation (EMR), can lead to changes in particular biological processes [1, 2]. In this study we investigated experimentally the hypothesis of the Resonant Recognition Model (RRM) that the selectivity of protein activities is based on specific resonant electromagnetic interactions [3]. The RRM theory proposes that an external electromagnetic field at a particular activation frequency would produce resonant effects on the biological activity of a particular protein, and this activation frequency can be determined computationally [3]. In our previous study [1], it was proposed that the wavelengths of the EMR in the range of 3500-4200nm are expected to affect biological activity of proto-oncogenic proteins [1, 4]. Thus, an exposure system based on Infrared Light Emitting Diodes (IR-LEDs) was constructed and used to irradiate mouse melanoma (B16F0) and Chinese Hamster Ovary (CHO) cell lines with the computationally determined far infrared wavelengths of 3400nm, 3600nm, 3800nm, 3900nm, 4100nm and 4300nm specific to mouse cells. In addition, the same cells, B16F10 and CHO, were exposed to visible and near infrared light radiation at the wavelengths of 466nm, 585nm, 626nm, 810nm, 850nm and 950nm. The results discussed here are obtained from lactate hydrogenate (LDH) cytotoxicity assay for all twelve studied wavelengths of visible and infrared light. A qualitative analysis has also been conducted using Light microscopy and the results from this study are presented and discussed

Experimental evaluation of cytotoxicity effects in cancer and normal cells exposed to far infrared radiation

It has been proven that many of biological processes are frequency selective pro- cesses that relate to quantum energy state of photosensitive molecules. It was shown that light- activated changes in protein energy states can induce or modulate biological processes. Various up-to-date methodologies that incorporate low-intensity light into therapeutic procedures have been integrated into modern medicine. Here we have studied experimentally the hypothesis of the Resonant Recognition Model (RRM) that selectivity of protein activities is based on speci¯c resonant electromagnetic interactions [1]. The RRM theory proposes that an external electro- magnetic ¯eld at a particular activation frequency would produce resonant e®ects on protein biological activity, and this activation frequency can be determined computationally [1]

The effects of synthetic azurocidin peptide analogue on staphylococcus aureus bacterium

Antibiotics are commonly used as anti-infection drugs. However, the rising of microbial resistance to antibiotics imposes a major challenge to their widespread applications. Hence, there is a growing need to find alternative drugs to eradicate the microbial resistance arising from the excessive use of antibiotics. Antimicrobial peptides (AMPs) are natural defence molecules found in human body. These AMP are present virtually in all life forms where they act as the first line defence agents against invading pathogens. Published studies suggest the possible use of AMPs as alternative anti-infective drugs. In this study we evaluated the anti-microbial activity of a synthetic Azurocidin peptide analogue and compared its efficacy with the native natural antimicrobial peptide Azurocidin. The Resonant Recognition Model (RRM) was employed here to computationally design a short Azurocidin peptide analogue, Azu-RRM. According to the RRM, this de novo designed peptide analogue will mimic and exhibit the activity of the natural Azurocidin (Azu) protein. Within this study the antimicrobial activity of Azu-RRM was investigated on Staphylococcus aureus (ATCC 25923) bacterium. The results obtained reveal that the synthetic peptide analogue affected the growth of this gram positive bacterium. The findings also showed that the Azu-RRM is exhibiting the anti-microbial effects on the growth of the studied bacteria comparable with the suppressing effects induced by the natural Azu protein