The use of phenyl-Sepharose for the affinity purification of proteinases

Phenyl-Sepharose is most often used as an adsorbent for hydrophobic interaction chromatography (HIC). We report on its effective use for the affinity purification of some extracellular thermostable proteinases from bacterial sources. Proteinases belonging to the serine, aspartate and metallo mechanistic classes were effective retained by the media. Purification factors in the range of 2.9–60 and enzyme activity yields in excess of 88% were obtained. In some cases homogeneous enzyme was obtained from culture supernatants in a single step. A number of other proteinases from mammalian sources were also retained. The specificity of the enzyme/support interaction was studied. Proteinases complexed with peptide inhibitors (pepstatin and chymostatin) showed reduced binding to phenyl Sepharose indicating with the active site cleft whereas modification with low molecular weight active site directed inactivators such as PMSF and DAN did not, indicating that binding may not be dependent on the catalytic site. Pepsinogen and the pro-enzyme form of the serine proteinase from the thermophilic Bacillus sp. strain Ak.1 were not retained by the media and could be resolved in an efficient manner from their active counterparts

Measurement of optical to electrical and electrical to optical delays with ps-level uncertainty

We present a new measurement principle to determine the absolute time delay of a waveform from an optical reference plane to an electrical reference plane and vice versa. We demonstrate a method based on this principle with 2 ps uncertainty. This method can be used to perform accurate time delay determinations of optical transceivers used in fibre-optic time-dissemination equipment. As a result the time scales in optical and electrical domain can be related to each other with the same uncertainty. We expect this method to break new grounds in high-accuracy time transfer and absolute calibration of time-transfer equipment

Exploring the use of a web-based virtual patient to support learning through reflection

This thesis explores the support of learning through reflection, in the context of medical students and practitioners, working through a series of simulated consultations involving the diagnosis and management of chronic illness. A model of the medical consultative process was defined, on which a web-based patient simulation was developed. This simulation can be accessed over the Internet using commonly available web-browsers. It enables users to interact with a virtual patient by taking a history, examining the patient, requesting and reviewing investigations, and choosing appropriate management strategies. The virtual patient can be reviewed over a number of consultations, and the patient outcome is dependant on the management strategy selected by the user. A second model was also developed, that adds a layer of reflection over the consultative process. While interacting with the virtual patient users are asked to formulate and test their hypotheses. Simple tools are included to encourage users to record their observations and thoughts for further learning, as well as providing links to web-based library resources. At the end of each consultation, users are asked to review their actions and indicate whether they think their actions were critical, relevant, or not relevant to the diagnosis and management of the patient in light of their current knowledge. Users also have the opportunity to compare their activity to their peers or an expert in the case under study. Three formal cycles of evaluation were undertaken during the design and development of the software. A number of clinicians were involved in the initial design to ensure there was an appropriate structure that matched clinical practice. Formative evaluation was conducted to review the usability of the application, and based on user feedback a number of changes were made to the user interface and structure of the application. A third, end user, evaluation was undertaken using a single case concerning the diagnosis and management of hypertriglyceridaemia in the context of Type 1B Glycogen Storage Disease. This evaluation involved ten medical students, five general practitioners and two specialists. The evaluation involved observation using a simplified think-aloud, as well as administration of a questionnaire. Users were engaged by the simulation, and were able to use the application with only a short period of training. Usability issues still exist with respect to the processing of natural language input, especially when asking questions of the virtual patient. Until such time that natural language recognition is able to provide satisfactory performance, alternative, list-based, methods of interaction will be required. Evaluation involving medical students, general practitioners, and specialist medical practitioners demonstrated that reflection can be supported and encouraged by providing appropriate tools, as well as by judiciously interrupting the consultative process and providing time for reflection to take place. Reflection could have been further enhanced if users had been educated on reflection as a learning modality prior to using SIMPRAC. Further work is also required to improve the simulation environment, improve the interfaces for supporting reflection, and further define the benefits of using this approach for medical education and professional development with respect to learning outcomes and behavioural change

Reconstructing Deconstruction: High-Velocity Cloud Distance Through Disruption Morphology

We present Arecibo L-band Feed Array 21-cm observations of a sub-complex of HVCs at the tip of the Anti-Center Complex. These observations show morphological details that point to interaction with the ambient halo medium and differential drag within the cloud sub-complex. We develop a new technique for measuring cloud distances, which relies upon these observed morphological and kinematic characteristics, and show that it is consistent with H-alpha distances. These results are consistent with distances to HVCs and halo densities derived from models in which HVCs are formed from cooling halo gas.Comment: 8 pages, 2 figures, 1 tabe, Accepted to Ap

Ongoing Galactic Accretion: Simulations and Observations of Condensed Gas in Hot Halos

Ongoing accretion onto galactic disks has been recently theorized to progress via the unstable cooling of the baryonic halo into condensed clouds. These clouds have been identified as analogous to the High-Velocity Clouds (HVCs) observed in HI in our Galaxy. Here we compare the distribution of HVCs observed around our own Galaxy and extra-planar gas around the Andromeda galaxy to these possible HVC analogs in a simulation of galaxy formation that naturally generates these condensed clouds. We find a very good correspondence between these observations and the simulation, in terms of number, angular size, velocity distribution, overall flux and flux distribution of the clouds. We show that condensed cloud accretion only accounts for ~ 0.2 M_solar / year of the current overall Galactic accretion in the simulations. We also find that the simulated halo clouds accelerate and become more massive as they fall toward the disk. The parameter space of the simulated clouds is consistent with all of the observed HVC complexes that have distance constraints, except the Magellanic Stream which is known to have a different origin. We also find that nearly half of these simulated halo clouds would be indistinguishable from lower-velocity gas and that this effect is strongest further from the disk of the galaxy, thus indicating a possible missing population of HVCs. These results indicate that the majority of HVCs are consistent with being infalling, condensed clouds that are a remnant of Galaxy formation.Comment: 10 pages, 6 figures, ApJ Accepted. Some changes to techniqu

Measuring the 8621 \r{A} Diffuse Interstellar Band in Gaia DR3 RVS Spectra: Obtaining a Clean Catalog by Marginalizing over Stellar Types

Diffuse interstellar bands (DIBs) are broad absorption features associated with interstellar dust and can serve as chemical and kinematic tracers. Conventional measurements of DIBs in stellar spectra are complicated by residuals between observations and best-fit stellar models. To overcome this, we simultaneously model the spectrum as a combination of stellar, dust, and residual components, with full posteriors on the joint distribution of the components. This decomposition is obtained by modeling each component as a draw from a high-dimensional Gaussian distribution in the data-space (the observed spectrum) -- a method we call "Marginalized Analytic Data-space Gaussian Inference for Component Separation" (MADGICS). We use a data-driven prior for the stellar component, which avoids missing stellar features not well-modeled by synthetic spectra. This technique provides statistically rigorous uncertainties and detection thresholds, which are required to work in the low signal-to-noise regime that is commonplace for dusty lines of sight. We reprocess all public Gaia DR3 RVS spectra and present an improved 8621 \r{A} DIB catalog, free of detectable stellar line contamination. We constrain the rest-frame wavelength to $8623.14 \pm 0.087$ \r{A} (vacuum), find no significant evidence for DIBs in the Local Bubble from the $1/6^{\rm{th}}$ of RVS spectra that are public, and show unprecedented correlation with kinematic substructure in Galactic CO maps. We validate the catalog, its reported uncertainties, and biases using synthetic injection tests. We believe MADGICS provides a viable path forward for large-scale spectral line measurements in the presence of complex spectral contamination.Comment: 25 pages, 25 figures, submitted to Ap

Compact HI clouds from the GALFA-HI survey

The Galactic Arecibo L-band Feed Array HI (GALFA-HI) survey is mapping the entire Arecibo sky at 21-cm, over a velocity range of -700 to +700 km/s (LSR), at a velocity resolution of 0.18 km/s and a spatial resolution of 3.5 arcmin. The unprecedented resolution and sensitivity of the GALFA-HI survey have resulted in the detection of numerous isolated, very compact HI clouds at low Galactic velocities, which are distinctly separated from the HI disk emission. In the limited area of ~4600 deg$^2$ surveyed so far, we have detected 96 of such compact clouds. The detected clouds are cold with a median T$_{k,max}$ (the kinetic temperature in the case in which there is no non-thermal broadening) of 300 K. Moreover, these clouds are quite compact and faint, with median values of 5 arcmin in angular size, 0.75 K in peak brightness temperature, and $5 \times 10^{18}$ cm$^{-2}$ in HI column density. Most of the clouds deviate from Galactic rotation at the 20-30 km/s level, and a significant fraction show evidence for a multiphase medium and velocity gradients. No counterparts for these clouds were found in other wavebands. From the modeling of spatial and velocity distributions of the whole compact cloud population, we find that the bulk of the compact clouds are related to the Galactic disk, and their distances are likely to be in the range of 0.1 to a few kpc. We discuss various possible scenarios for the formation and maintenance of this cloud population and its significance for Galactic ISM studies.Comment: Accepted for publication in the Astrophysical Journa

Multiphysics simulation of corona discharge induced ionic wind

Ionic wind devices or electrostatic fluid accelerators are becoming of increasing interest as tools for thermal management, in particular for semiconductor devices. In this work, we present a numerical model for predicting the performance of such devices, whose main benefit is the ability to accurately predict the amount of charge injected at the corona electrode. Our multiphysics numerical model consists of a highly nonlinear strongly coupled set of PDEs including the Navier-Stokes equations for fluid flow, Poisson's equation for electrostatic potential, charge continuity and heat transfer equations. To solve this system we employ a staggered solution algorithm that generalizes Gummel's algorithm for charge transport in semiconductors. Predictions of our simulations are validated by comparison with experimental measurements and are shown to closely match. Finally, our simulation tool is used to estimate the effectiveness of the design of an electrohydrodynamic cooling apparatus for power electronics applications.Comment: 24 pages, 17 figure

The Effelsberg-Bonn HI Survey: Data reduction

Starting in winter 2008/2009 an L-band 7-Feed-Array receiver is used for a 21-cm line survey performed with the 100-m telescope, the Effelsberg-Bonn HI survey (EBHIS). The EBHIS will cover the whole northern hemisphere for decl.>-5 deg comprising both the galactic and extragalactic sky out to a distance of about 230 Mpc. Using state-of-the-art FPGA-based digital fast Fourier transform spectrometers, superior in dynamic range and temporal resolution to conventional correlators, allows us to apply sophisticated radio frequency interference (RFI) mitigation schemes. In this paper, the EBHIS data reduction package and first results are presented. The reduction software consists of RFI detection schemes, flux and gain-curve calibration, stray-radiation removal, baseline fitting, and finally the gridding to produce data cubes. The whole software chain is successfully tested using multi-feed data toward many smaller test fields (1--100 square degrees) and recently applied for the first time to data of two large sky areas, each covering about 2000 square degrees. The first large area is toward the northern galactic pole and the second one toward the northern tip of the Magellanic Leading Arm. Here, we demonstrate the data quality of EBHIS Milky Way data and give a first impression on the first data release in 2011.Comment: 17 pages, 14 figures; to be published in ApJ