2MASS J05162881+2607387: A New Low-Mass Double-Lined Eclipsing Binary

We show that the star known as 2MASS J05162881+2607387 (hereafter J0516) is a double-lined eclipsing binary with nearly identical low-mass components. The spectroscopic elements derived from 18 spectra obtained with the High Resolution Spectrograph on the Hobby-Eberly Telescope during the Fall of 2005 are K_1=88.45 +/- 0.48 km/s and K_2=90.43 +/- 0.60 km/s, resulting in a mass ratio of$q=K_1/K_2 = 0.978 +/- 0.018 and minimum masses of M_1 sin^{3}i=0.775 +/- 0.016 solar masses and M_2 sin^{3}i=0.759 +/- 0.012 solar masses, respectively. We have extensive differential photometry of J0516 obtained over several nights between 2004 January-March (epoch 1) and 2004 October-2005 January plus 2006 January (epoch 2) using the 1m telescope at the Mount Laguna Observatory. The source was roughly 0.1 mag brighter in all three bandpasses during epoch 1 when compared to epoch 2. Also, phased light curves from epoch 1 show considerable out-of-eclipse variability, presumably due to bright spots on one or both stars. In contrast, the phased light curves from epoch 2 show little out-of-eclipse variability. The light curves from epoch 2 and the radial velocity curves were analyzed using our ELC code with updated model atmospheres for low-mass stars. We find the following: M_1=0.787 +/- 0.012 solar masses, R_1=0.788 +/- 0.015 solar radii, M_2=0.770 +/- 0.009 solar masses, and R_2=0.817 +/- 0.010 solar radii. The stars in J0516 have radii that are significantly larger than model predictions for their masses, similar to what is seen in a handful of other well-studied low-mass double-lined eclipsing binaries. We compiled all recent mass and radius determinations from low-mass binaries and determine an empirical mass-radius relation of the form R = 0.0324 + 0.9343M + 0.0374M^2, where the quantities are in solar units.Comment: 16 pages, 10 figures (Figure 1 has degraded quality), to appear in Ap

Identification of Cohesive Ends and Genes Encoding the Terminase of Phage 16-3

Cohesive ends of 16-3, a temperate phage of Rhizobium meliloti 41, have been identified as 10-base-long, 3′-protruding complementary G/C-rich sequences. terS and terL encode the two subunits of 16-3 terminase. Significant homologies were detected among the terminase subunits of phage 16-3 and other phages from various ecosystems

Enhancement of experimental metastasis by tumor necrosis factor

The influence of endogenous and exogenous tumor necrosis factor (TNF) on metastasis was investigated in an experimental fibrosarcoma metastasis model. A single intraperitoneal injection of recombinant human (rh) TNF or recombinant mouse (rm) TNF into mice 5 h before intravenous inoculation of methylcholanthrene-induced fibrosarcoma cells (CFS1) induced a significant enhancement of the number of metastases in the lung. Dose responses of rmTNF and rhTNF demonstrated a stronger metastasis-augmenting effect by rmTNF compared with rhTNF. This effect was time dependent, as administration of rmTNF 5 h before or 1 h but not 24 h after tumor cell inoculation caused an increase of tumor cell colony formation on the lung surface, suggesting an influence of TNF on the vascular adhesion and diapedesis of tumor cells. Since tumor-bearing mice showed an enhanced ability to produce TNF after endotoxin injection compared to control mice, tumor-bearing mice were treated with anti-mTNF antibodies. Neutralization of endogenous tumor-induced TNF led to a significant decrease of the number of pulmonary metastases. Histological analysis of micrometastases in the lung on day 5 by silver staining of proteins associated with nucleolar organizer regions revealed more metastatic foci and augmented proliferative activity of the tumor cells after rmTNF pretreatment of mice. However, no direct effect of rmTNF on the proliferation rate of tumor cells was seen in vitro. These findings suggest that low doses of endogenous TNF or administered TNF during cytokine therapy might enhance the metastatic potential of circulating tumor cells

The Influence of Specimen Thickness on the High Temperature Corrosion Behavior of CMSX-4 during Thermal-Cycling Exposure

CMSX-4 is a single-crystalline Ni-base superalloy designed to be used at very high temperatures and high mechanical loadings. Its excellent corrosion resistance is due to external alumina-scale formation, which however can become less protective under thermal-cycling conditions. The metallic substrate in combination with its superficial oxide scale has to be considered as a composite suffering high stresses. Factors like different coefficients of thermal expansion between oxide and substrate during temperature changes or growing stresses affect the integrity of the oxide scale. This must also be strongly influenced by the thickness of the oxide scale and the substrate as well as the ability to relief such stresses, e.g., by creep deformation. In order to quantify these effects, thin-walled specimens of different thickness (t = 100500 lm) were prepared. Discontinuous measurements of their mass changes were carried out under thermal-cycling conditions at a hot dwell temperature of 1100 C up to 300 thermal cycles. Thin-walled specimens revealed a much lower oxide-spallation rate compared to thick-walled specimens, while thinwalled specimens might show a premature depletion of scale-forming elements. In order to determine which of these competetive factor is more detrimental in terms of a component’s lifetime, the degradation by internal precipitation was studied using scanning electron microscopy (SEM) in combination with energy-dispersive X-ray spectroscopy (EDS). Additionally, a recently developed statistical spallation model was applied to experimental data [D. Poquillon and D. Monceau, Oxidation of Metals, 59, 409–431 (2003)]. The model describes the overall mass change by oxide scale spallation during thermal cycling exposure and is a useful simulation tool for oxide scale spallation processes accounting for variations in the specimen geometry. The evolution of the net-mass change vs. the number of thermal cycles seems to be strongly dependent on the sample thickness

AREA WIDE TRAFFIC DEMAND MANAGEMENT BY ROAD PRICING

Cities and governments spent a tremendous amount of money to ease traffic congestion, but the problems are yet unsolved. The difficulties are well-known: increase of costs and time spent on travelling in cities; noise nuisance, air pollution, vibration; increase of urban accident rates; deforming land use patterns. Recent research suggests to apply some form of Road Pricing as an important tool among many others to manage demand on motorized traffic. Road Pricing may be effective as it reduces costs of traffic; regulates and reduces car use in peak hours and pricing contributes to maintaining better environmental and living conditions in city centres. Road Pricing system should be included within a comprehensive Land Use and Transport Policy. Car ownership in Budapest will double to about 400 cars/l000 inhabitants within 30 years. It seems clear that some regulation and restriction on car use cannot be avoided. Simultaneous, coordinated elements of traffic demand management in Budapest may be the following: land use policy: development of public transport; area wide traffic control and route guidance systems: parking policy; other instruments and Road Pricing as a major tool to manage demand for car traffic. There are several reasonable solutions how to locate charging stations in Budapest. In the far future it seems necessary to charge drivers entering the city area within the M0 circular motorway. In the first phase outer cordon may be established in line of 'Hungarian Ringroad' after its southern end with 'Lágymányosi Bridge' across the River Danube will have been completed. On the 'Buda' side cordon can be put on the ring road (that cannot be so complete as on the 'Pest' side), and additional charging stations can be located on the Danube Bridges. This preliminary suggestion must be controlled and changed by results of precise and detailed application of traffic models, and interdisciplinary preparation

Refined Neutron-Star Mass Determinations for Six Eclipsing X-Ray Pulsar Binaries

We present an improved method for determining the mass of neutron stars in eclipsing X-ray pulsar binaries and apply the method to six systems, namely Vela X-1, 4U 1538-52, SMC X-1, LMC X-4, Cen X-3, and Her X-1. In previous studies to determine neutron star mass, the X-ray eclipse duration has been approximated analytically by assuming the companion star is spherical with an effective Roche lobe radius. We use a numerical code based on Roche geometry with various optimizers to analyze the published data for these systems, which we supplement with new spectroscopic and photometric data for 4U 1538-52. This allows us to model the eclipse duration more accurately and thus calculate an improved value for the neutron star mass. The derived neutron star mass also depends on the assumed Roche lobe filling factor beta of the companion star, where beta = 1 indicates a completely filled Roche lobe. In previous work a range of beta between 0.9 and 1.0 was usually adopted. We use optical ellipsoidal lightcurve data to constrain beta. We find neutron star masses of 1.77 +/- 0.08 M_{sun} for Vela X-1, 0.87 +/- 0.07 M_{sun} for 4U 1538-52 (eccentric orbit), 1.00 +/- 0.10 M_{sun} for 4U 1538-52 (circular orbit), 1.04 +/- 0.09 M_{sun} for SMC X-1, 1.29 +/- 0.05 M_{sun} for LMC X-4, 1.49 +/- 0.08 M_{sun} for Cen X-3, and 1.07 +/- 0.36 M_{sun} for Her X-1. We discuss the limits of the approximations that were used to derive the earlier mass determinations, and we comment on the implications our new masses have for observationally refining the upper and lower bounds of the neutron star mass distribution.Comment: 10 figures, accepted for publication in The Astrophysical Journa

Calpastatin Subdomains A And C Are Activators of Calpain

The inhibitory domains of calpastatin contain three highly conserved regions, A, B, and C, of which A and C bind calpain in a strictly Ca2+-dependent manner but have no inhibitory activity whereas region B inhibits calpain on its own. We synthesized the 19-mer oligopeptides corresponding to regions A and C of human calpastatin domain I and tested their effect on human erythrocyte mu-calpain and rat m-calpain. The two peptides significantly activate both calpains: the Ca2+ concentration required for half-maximal activity is lowered from 4.3 to 2.4 mum for mu-calpain and from 250 to 140 mum for m-calpain. The EC50 concentration of the peptides is 7.5 mum for mu-calpain and 25 mum for m-calpain. It is noteworthy that at low Ca2+ concentrations (1-2 mum for mu-calpain and 70-110 mum for m-calpain) both enzymes are activated about 10-fold by the peptides. Based on these findings, it is suggested that calpastatin fragments may have a role in calpain activation in vivo. Furthermore, these activators open new avenues to cell biological studies of calpain function and eventually may alleviate pathological states caused by calpain malfunction