Environmental heat stress offsets adaptation associated with carbohydrate periodization in trained male triathletes

Purpose: Carbohydrate (CHO) intake periodization via the sleep low train low (SL-TL) diet–exercise model increases fat oxidation during exercise and may enhance endurance-training adaptation and performance. Conversely, training under environmental heat stress increases CHO oxidation, but the potential of combined SL-TL and heat stress to enhance metabolic and performance outcomes is unknown. Methods: Twenty-three endurance-trained males were randomly assigned to either control (n = 7, CON), SL-TL (n = 8, SLTemp) or SL-TL + heat stress (n = 8, SLHeat) groups and prescribed identical 2-week cycling training interventions. CON and SLTemp completed all sessions at 20°C, but SLHeat at 35°C. All groups consumed matched CHO intake (6 g·kg−1·day−1) but timed differently to promote low CHO availability overnight and during morning exercise in both SL groups. Submaximal substrate utilization was assessed (at 20°C), and 30-min performance tests (at 20 and 35°C) were performed Pre-, Post-, and 1-week post-intervention (Post+1). Results: SLTemp improved fat oxidation rates at 60% MAP (~66% VO2peak) at Post+1 compared with CON (p < 0.01). Compared with SLTemp, fat oxidation rates were significantly lower in SLHeat at Post (p = 0.02) and Post+1 (p < 0.05). Compared with CON, performance was improved at Post in SLTemp in temperate conditions. Performance was not different between any groups or time points in hot conditions. Conclusion: SL-TL enhanced metabolic adaptation and performance compared with CON and combined SL-TL and heat stress. Additional environmental heat stress may impair positive adaptations associated with SL-TL

Acute heat stress amplifies exercise‐induced metabolomic perturbations and reveals variation in circulating amino acids in endurance‐trained males

Using untargeted metabolomics, we aimed to characterise the systemic impact of environmental heat stress during exercise. Twenty-three trained male triathletes (◂◽.▸ = 64.8 ± 9.2 ml kg min−1) completed a 30-min exercise test in hot (35°C) and temperate (21°C) conditions. Venous blood samples were collected immediately pre- and post-exercise, and the serum fraction was assessed via untargeted 1H-NMR metabolomics. Data were analysed via uni- and multivariate analyses to identify differences between conditions. Mean power output was higher in temperate (231 ± 36 W) versus hot (223 ± 31 W) conditions (P 0.05). Environmental heat stress increased glycolytic metabolite abundance and led to distinct alterations in the circulating amino acid availability, including increased alanine, glutamine, leucine and isoleucine. The data highlight the need for additional exercise nutrition and metabolism research, specifically focusing on protein requirements for exercise under heat stress

Formal Analysis of Quantum Systems using Process Calculus

Quantum communication and cryptographic protocols are well on the way to becoming an important practical technology. Although a large amount of successful research has been done on proving their correctness, most of this work does not make use of familiar techniques from formal methods, such as formal logics for specification, formal modelling languages, separation of levels of abstraction, and compositional analysis. We argue that these techniques will be necessary for the analysis of large-scale systems that combine quantum and classical components, and summarize the results of initial investigation using behavioural equivalence in process calculus. This paper is a summary of Simon Gay's invited talk at ICE'11.Comment: In Proceedings ICE 2011, arXiv:1108.014

Seascape Genetics and the Spatial Ecology of Juvenile Green Turtles

WOS:000529189000042International audienceUnderstanding how ocean currents impact the distribution and connectivity of marine species, provides vital information for the effective conservation management of migratory marine animals. Here, we used a combination of molecular genetics and ocean drift simulations to investigate the spatial ecology of juvenile green turtle (Chelonia mydas) developmental habitats, and assess the role of ocean currents in driving the dispersal of green turtle hatchlings. We analyzed mitochondrial (mt)DNA sequenced from 358 juvenile green turtles, and from eight developmental areas located throughout the Southwest Indian Ocean (SWIO). A mixed stock analysis (MSA) was applied to estimate the level of connectivity between developmental sites and published genetic data from 38 known genetic stocks. The MSA showed that the juvenile turtles at all sites originated almost exclusively from the three known SWIO stocks, with a clear shift in stock contributions between sites in the South and Central Areas. The results from the genetic analysis could largely be explained by regional current patterns, as shown by the results of passive numerical drift simulations linking breeding sites to developmental areas utilized by juvenile green turtles. Integrating genetic and oceanographic data helps researchers to better understand how marine species interact with ocean currents at different stages of their lifecycle, and provides the scientific basis for effective conservation management

Acute heat stress amplifies exercise-induced metabolomic perturbations and reveals variation in circulating amino acids in endurance-trained males.

New findingsWhat is the central question of this study? Whole-body substrate utilisation is altered during exercise in hot environments, characterised by increased glycolytic metabolism: does heat stress alter the serum metabolome in response to high intensity exercise? What are the main finding and its importance? Alongside increases in glycolytic metabolite abundance, circulating amino acid concentrations are reduced following exercise under heat stress. Prior research has overlooked the impact of heat stress on protein metabolism during exercise, raising important practical implications for protein intake recommendations in the heat.AbstractUsing untargeted metabolomics, we aimed to characterise the systemic impact of environmental heat stress during exercise. Twenty-three trained male triathletes ( V̇O2peak${\dot V_{{{\rm{O}}_2}{\rm{peak}}}}$  = 64.8 ± 9.2 ml kg min-1 ) completed a 30-min exercise test in hot (35°C) and temperate (21°C) conditions. Venous blood samples were collected immediately pre- and post-exercise, and the serum fraction was assessed via untargeted 1 H-NMR metabolomics. Data were analysed via uni- and multivariate analyses to identify differences between conditions. Mean power output was higher in temperate (231 ± 36 W) versus hot (223 ± 31 W) conditions (P -1 , hot, 167 ± 9 beats min-1 , P rec ), core temperature change (ΔTrec ) (P  0.05). Environmental heat stress increased glycolytic metabolite abundance and led to distinct alterations in the circulating amino acid availability, including increased alanine, glutamine, leucine and isoleucine. The data highlight the need for additional exercise nutrition and metabolism research, specifically focusing on protein requirements for exercise under heat stress