The effect of current Schistosoma mansoni infection on the immunogenicity of a candidate TB vaccine, MVA85A, in BCG-vaccinated adolescents: An open-label trial.

INTRODUCTION: Helminth infection may affect vaccine immunogenicity and efficacy. Adolescents, a target population for tuberculosis booster vaccines, often have a high helminth burden. We investigated effects of Schistosoma mansoni (Sm) on the immunogenicity and safety of MVA85A, a model candidate tuberculosis vaccine, in BCG-vaccinated Ugandan adolescents. METHODS: In this phase II open label trial we enrolled 36 healthy, previously BCG-vaccinated adolescents, 18 with no helminth infection detected, 18 with Sm only. The primary outcome was immunogenicity measured by Ag85A-specific interferon gamma ELISpot assay. Tuberculosis and schistosome-specific responses were also assessed by whole-blood stimulation and multiplex cytokine assay, and by antibody ELISAs. RESULTS: Ag85A-specific cellular responses increased significantly following immunisation but with no differences between the two groups. Sm infection was associated with higher pre-immunisation Ag85A-specific IgG4 but with no change in antibody levels following immunisation. There were no serious adverse events. Most reactogenicity events were of mild or moderate severity and resolved quickly. CONCLUSIONS: The significant Ag85A-specific T cell responses and lack of difference between Sm-infected and uninfected participants is encouraging for tuberculosis vaccine development. The implications of pre-existing Ag85A-specific IgG4 antibodies for protective immunity against tuberculosis among those infected with Sm are not known. MVA85A was safe in this population. TRIAL REGISTRATION: ClinicalTrials.gov NCT02178748

Influence of species/cultivar and season on the quality of Atriplex grown at different sites in South Africa

Abstract Atriplex species/cultivars [Atriplex nummularia, A. canescens (Santa Rita) and A. canescens (Field Reserve 1)] were compared in terms of chemical composition and in vitro digestibility. The plant material was sampled at different seasons (autumn and winter) and from different sites (Hatfield in the Gauteng Province and Mier and Lovedale, both in the Northern Cape Province) and were analysed for crude protein (CP), neutral detergent fibre (NDF) and in vitro digestibility (IVOMD). The CP and IVOMD of the leaves were higher than the stem for the Atriplex species. Leaf percentage for the autumn samples was not affected by species at Hatfield and Mier, but A. canescens (Field Reserve 1) had lower leaf percentage at Lovedale as well as for winter samples at Hatfield. At Lovedale, however, A. nummularia had the highest leaf : stem ratio compared to A. canescens (Santa Rita) and A. canescens (Field Reserve 1). Atriplex nummularia had a higher CP concentration than A. canescens (Field Reserve 1) and A. canescens (Santa Rita) at both Mier and Lovedale, but species/cultivar had no effect on CP concentration at Hatfield. Autumn samples had a higher CP concentration than winter samples at both Mier and Lovedale, but season had no effect on the N concentration at Hatfield. Atriplex nummularia had a lower NDF and higher IVOMD concentration compared to A. canescens (Santa Rita) and A. canescens (Field Reserve 1). Autumn samples were less fibrous and more digestible than winter samples. Atriplex nummularia seems to be better in terms of its nutritive value compared to A. canescens (Santa Rita) and A. canescens (Field Reserve 1), with better quality forage in autumn than winter. _______________________________________________________________________________

A Complete Set of Solutions For Caustic-Crossing Binary Microlensing Events

We present a method to analyze binary-lens microlensing light curves with one well-sampled fold caustic crossing. In general, the surface of chi^2 shows extremely complicated behavior over the 9-parameter space that characterizes binary lenses. This makes it difficult to systematically search the space and verify that a given local minimum is a global minimum. We show that for events with well-monitored caustics, the caustic-crossing region can be isolated from the rest of the light curve and easily fit to a 5-parameter function. Four of these caustic-crossing parameters can then be used to constrain the search in the larger 9-parameter space. This allows a systematic search for all solutions and thus identification of all local minima. We illustrate this technique using the PLANET data for MACHO 98-SMC-1, an excellent and publicly available caustic-crossing data set. We show that a very broad range of parameter combinations are compatible with the PLANET data set, demonstrating that observations of binary-lens lightcurves with sampling of only one caustic crossing do not yield unique solutions. The corollary to this is that the time of the second caustic crossing cannot be reliably predicted on the basis of early data including the first caustic crossing alone. We investigate the requirements for determination of a unique solution and find that occasional observations of the first caustic crossing may be sufficient to derive a complete solution.Comment: 31 pages + 8 figures + 2 table

Limits on Stellar and Planetary Companions in Microlensing Event OGLE-1998-BUL-14

We present the PLANET photometric data set for \ob14, a high magnification ($A_{\rm max}\sim 16$) event alerted by the OGLE collaboration toward the Galactic bulge in 1998. The PLANET data set consists a total of 461 I-band and 139 $V-$band points, the majority of which was taken over a three month period. The median sampling interval during this period is about 1 hour, and the $1\sigma$ scatter over the peak of the event is 1.5%. The excellent data quality and high maximum magnification of this event make it a prime candidate to search for the short duration, low amplitude perturbations that are signatures of a planetary companion orbiting the primary lens. The observed light curve for \ob14 is consistent with a single lens (no companion) within photometric uncertainties. We calculate the detection efficiency of the light curve to lensing companions as a function of the mass ratio and angular separation of the two components. We find that companions of mass ratio $\ge 0.01$ are ruled out at the 95% confidence level for projected separations between 0.4-2.4 \re, where \re is the Einstein ring radius of the primary lens. Assuming that the primary is a G-dwarf with \re\sim3 {\rm AU} our detection efficiency for this event is $\sim 60$ for a companion with the mass and separation of Jupiter and $\sim5$ for a companion with the mass and separation of Saturn. Our efficiencies for planets like those around Upsilon And and 14 Her are > 75%.Comment: Data available at http://www.astro.rug.nl/~planet/planetpapers.html 20 pages, 10 figures. Minor changes. ApJ, accepte

Discovery of the optical counterpart and early optical observations of GRB990712

We present the discovery observations of the optical counterpart of the gamma-ray burster GRB990712 taken 4.16 hours after the outburst and discuss its light curve observed in the V, R and I bands during the first ~35 days after the outburst. The observed light curves were fitted with a power-law decay for the optical transient (OT), plus an additional component which was treated in two different ways. First, the additional component was assumed to be an underlying galaxy of constant brightness. The resulting slope of the decay is 0.97+/-0.05 and the magnitudes of the underlying galaxy are: V = 22.3 +/- 0.05, R = 21.75 +/- 0.05 and I = 21.35 +/- 0.05. Second, the additional component was assumed to be a galaxy plus an underlying supernova with a time-variable brightness identical to that of GRB980425, appropriately scaled to the redshift of GRB990712. The resulting slope of the decay is similar, but the goodness-of-fit is worse which would imply that either this GRB is not associated with an underlying supernova or the underlying supernova is much fainter than the supernova associated with GRB980425. The galaxy in this case is fainter: V = 22.7 +/- 0.05, R = 22.25 +/- 0.05 and I = 22.15 +/- 0.05; and the OT plus the underlying supernova at a given time is brighter. Measurements of the brightnesses of the OT and the galaxy by late-time HST observation and ground-based observations can thus assess the presence of an underlying supernova.Comment: To appear in Ap

Limb-Darkening of a K Giant in the Galactic Bulge: PLANET Photometry of MACHO 97-BLG-28

We present the PLANET photometric dataset for the binary-lens microlensing event MACHO 97-BLG-28 consisting of 696 I and V-band measurements, and analyze it to determine the radial surface brightness profile of the Galactic bulge source star. The microlensed source, demonstrated to be a K giant by our independent spectroscopy, crossed the central isolated cusp of the lensing binary, generating a sharp peak in the light curve that was well-resolved by dense (3 - 30 minute) and continuous monitoring from PLANET sites in Chile, South Africa, and Australia. Our modeling of these data has produced stellar profiles for the source star in the I and V bands that are in excellent agreement with those predicted by stellar atmospheric models for K giants. The limb-darkening coefficients presented here are the first derived from microlensing, among the first for normal giants by any technique, and the first for any star as distant as the Galactic bulge. Modeling indicates that the lensing binary has a mass ratio q = 0.23 and an (instantaneous) separation in units of the angular Einstein ring radius of d = 0.69 . For a lens in the Galactic bulge, this corresponds to a typical stellar binary with a projected separation between 1 and 2 AU. If the lens lies closer, the separation is smaller, and one or both of the lens objects is in the brown dwarf regime. Assuming that the source is a bulge K2 giant at 8 kpc, the relative lens-source proper motion is mu = 19.4 +/- 2.6 km/s /kpc, consistent with a disk or bulge lens. If the non-lensed blended light is due to a single star, it is likely to be a young white dwarf in the bulge, consistent with the blended light coming from the lens itself.Comment: 32 Pages, including 1 table and 9 postscript figures. (Revised version has slightly modified text, corrected typo, and 1 new figure.) Accepted for publication in 1999 Astrophysical Journal; data are now available at http://www.astro.rug.nl/~plane

Sub-threshold resonances in few-neutron systems

Three- and four-neutron systems are studied within the framework of the hyperspherical approach with a local S-wave nn-potential. Possible bound and resonant states of these systems are sought as zeros of three- and four-body Jost functions in the complex momentum plane. It is found that zeros closest to the origin correspond to sub-threshold (nnn) (1/2-) and (nnnn) (0+) resonant states. The positions of these zeros turned out to be sensitive to the choice of the $nn$--potential. For the Malfliet- Tjon potential they are E(nnn)=-4.9-i6.9 (MeV) and E(nnnn)=-2.6-i9.0 (MeV). Movement of the zeros with an artificial increase of the potential strength also shows an extreme sensitivity to the choice of potential. Thus, to generate ^3n and ^4n bound states, the Yukawa potential needs to be multiplied by 2.67 and 2.32 respectively, while for the Malfliet-Tjon potential the required multiplicative factors are 4.04 and 3.59.Comment: Latex, 22 pages, no PS-figures, submitted to J.Phys.

H-alpha Equivalent Width Variations across the Face of a Microlensed K Giant in the Galactic Bulge

We present VLT FORS1 spectroscopy that temporally resolves the second caustic crossing of the Bulge K giant source of microlensing event EROS 2000-BLG-5, the first time this has been accomplished for several phases of a caustic transit. The ~1 angstrom H-alpha equivalent width of the source star increases slightly as the center of the star egresses the caustic and then plummets by 30% during the final limb crossing. These changes are not seen in contemporaneous spectra of control stars in the FORS1 slit, but are qualitatively consistent with expectations from stellar atmosphere models as the caustic differentially magnifies different portions of the stellar face of the target. Observations such as these in a variety of stellar lines are equivalent to atmospheric tomography and are expected to provide a direct test of stellar models.Comment: 15 pages, including 1 table and 4 figures. As accepted by ApJ Letters, vol 55