31 research outputs found
ΠΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΡ ΡΠ°Π±ΠΎΡ ΠΏΠΎ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΈ Π»ΠΈΠΊΠ²ΠΈΠ΄Π°ΡΠΈΠΈ Π°Π²Π°ΡΠΈΠΉΠ½ΠΎΠ³ΠΎ ΡΠ°Π·Π»ΠΈΠ²Π° Π½Π΅ΡΡΠΈ ΠΈ Π½Π΅ΡΡΠ΅ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ² Π½Π° Π²ΠΎΠ΄Π½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠΈ
ΠΠ±ΡΠ΅ΠΊΡΠΎΠΌ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠ²Π»ΡΡΡΡΡ ΠΌΠ΅ΡΠΎΠ΄Ρ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΈ Π»ΠΈΠΊΠ²ΠΈΠ΄Π°ΡΠΈΠΈ ΡΠ°Π·Π»ΠΈΠ²ΠΎΠ² Π½Π΅ΡΡΠΈ Π½Π° Π²ΠΎΠ΄Π½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ. Π¦Π΅Π»Ρ ΡΠ°Π±ΠΎΡΡ β ΠΏΡΠΎΠ²Π΅ΡΡΠΈ Π°Π½Π°Π»ΠΈΠ· ΡΡΡΠ΅ΡΡΠ²ΡΡΡΠΈΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΠΈ ΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΌΠ΅ΡΠΎΠΏΡΠΈΡΡΠΈΠΉ ΠΏΠΎ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΈ Π»ΠΈΠΊΠ²ΠΈΠ΄Π°ΡΠΈΠΈ ΡΠ°Π·Π»ΠΈΠ²ΠΎΠ² Π½Π΅ΡΡΠΈ Π½Π° Π²ΠΎΠ΄Π½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ.
Π ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΡΡ Π°Π½Π°Π»ΠΈΠ· ΠΈΠΌΠ΅ΡΡΠΈΡ
ΡΡ Π΄Π°Π½Π½ΡΡ
, Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΊΠΎΡΠΎΡΡΡ
Π±ΡΠ»ΠΈ Π²ΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΈ Π»ΠΈΠΊΠ²ΠΈΠ΄Π°ΡΠΈΠΈ ΡΠ°Π·Π»ΠΈΠ²ΠΎΠ² Π½Π΅ΡΡΠΈ Π½Π° Π²ΠΎΠ΄Π½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ ΡΠ°ΡΡΠ΅Ρ ΠΎΠ±ΡΠ΅ΠΌΠ° Π²ΡΡΠ΅ΠΊΡΠ΅ΠΉ Π½Π΅ΡΡΠΈ ΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° ΡΠΎΡΠ±Π΅Π½ΡΠ°, ΡΡΠ΅Π±ΡΠ΅ΠΌΠΎΠ³ΠΎ Π΄Π»Ρ Π»ΠΈΠΊΠ²ΠΈΠ΄Π°ΡΠΈΠΈ ΡΠ°Π·Π»ΠΈΠ²Π°. Π ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ»ΠΎ Π²ΡΡΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌ ΡΠΏΠΎΡΠΎΠ±ΠΎΠΌ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΡΠ°Π·Π»ΠΈΠ²ΠΎΠ² Π½Π° Π²ΠΎΠ΄Π½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΡΠ²Π»ΡΡΡΡΡ Π±ΠΎΠ½Ρ Ρ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌΠΈ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΠ°ΠΌΠΈ ΠΈΡ
ΡΡΡΠ°Π½ΠΎΠ²ΠΊΠΈ, Π° Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ Π»ΠΈΠΊΠ²ΠΈΠ΄Π°ΡΠΈΠΈ β ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΊΠΈΠΌΠΌΠ΅ΡΠΎΠ² ΠΈ ΡΠΎΡΠ±Π΅Π½ΡΠΎΠ².ΠΠ±ΡΠ΅ΠΊΡΠΎΠΌ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠ²Π»ΡΡΡΡΡ ΠΌΠ΅ΡΠΎΠ΄Ρ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΈ Π»ΠΈΠΊΠ²ΠΈΠ΄Π°ΡΠΈΠΈ ΡΠ°Π·Π»ΠΈΠ²ΠΎΠ² Π½Π΅ΡΡΠΈ Π½Π° Π²ΠΎΠ΄Π½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ. Π¦Π΅Π»Ρ ΡΠ°Π±ΠΎΡΡ β ΠΏΡΠΎΠ²Π΅ΡΡΠΈ Π°Π½Π°Π»ΠΈΠ· ΡΡΡΠ΅ΡΡΠ²ΡΡΡΠΈΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΠΈ ΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΌΠ΅ΡΠΎΠΏΡΠΈΡΡΠΈΠΉ ΠΏΠΎ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΈ Π»ΠΈΠΊΠ²ΠΈΠ΄Π°ΡΠΈΠΈ ΡΠ°Π·Π»ΠΈΠ²ΠΎΠ² Π½Π΅ΡΡΠΈ Π½Π° Π²ΠΎΠ΄Π½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ.
Π ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΡΡ Π°Π½Π°Π»ΠΈΠ· ΠΈΠΌΠ΅ΡΡΠΈΡ
ΡΡ Π΄Π°Π½Π½ΡΡ
, Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΊΠΎΡΠΎΡΡΡ
Π±ΡΠ»ΠΈ Π²ΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΈ Π»ΠΈΠΊΠ²ΠΈΠ΄Π°ΡΠΈΠΈ ΡΠ°Π·Π»ΠΈΠ²ΠΎΠ² Π½Π΅ΡΡΠΈ Π½Π° Π²ΠΎΠ΄Π½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ ΡΠ°ΡΡΠ΅Ρ ΠΎΠ±ΡΠ΅ΠΌΠ° Π²ΡΡΠ΅ΠΊΡΠ΅ΠΉ Π½Π΅ΡΡΠΈ ΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° ΡΠΎΡΠ±Π΅Π½ΡΠ°, ΡΡΠ΅Π±ΡΠ΅ΠΌΠΎΠ³ΠΎ Π΄Π»Ρ Π»ΠΈΠΊΠ²ΠΈΠ΄Π°ΡΠΈΠΈ ΡΠ°Π·Π»ΠΈΠ²Π°. Π ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ»ΠΎ Π²ΡΡΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌ ΡΠΏΠΎΡΠΎΠ±ΠΎΠΌ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΡΠ°Π·Π»ΠΈΠ²ΠΎΠ² Π½Π° Π²ΠΎΠ΄Π½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΡΠ²Π»ΡΡΡΡΡ Π±ΠΎΠ½Ρ Ρ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌΠΈ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΠ°ΠΌΠΈ ΠΈΡ
ΡΡΡΠ°Π½ΠΎΠ²ΠΊΠΈ, Π° Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ Π»ΠΈΠΊΠ²ΠΈΠ΄Π°ΡΠΈΠΈ β ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΊΠΈΠΌΠΌΠ΅ΡΠΎΠ² ΠΈ ΡΠΎΡΠ±Π΅Π½ΡΠΎΠ²
A Crucial Role of Activin A-Mediated Growth Hormone Suppression in Mouse and Human Heart Failure
Infusion of bone marrow-derived mononuclear cells (BMMNC) has been reported to ameliorate cardiac dysfunction after acute myocardial infarction. In this study, we investigated whether infusion of BMMNC is also effective for non-ischemic heart failure model mice and the underlying mechanisms. Intravenous infusion of BMMNC showed transient cardioprotective effects on animal models with dilated cardiomyopathy (DCM) without their engraftment in heart, suggesting that BMMNC infusion improves cardiac function via humoral factors rather than their differentiation into cardiomyocytes. Using conditioned media from sorted BMMNC, we found that the cardioprotective effects were mediated by growth hormone (GH) secreted from myeloid (Gr-1(+)) cells and the effects was partially mediated by signal transducer and activator of transcription 3 in cardiomyocytes. On the other hand, the GH expression in Gr-1(+) cells was significantly downregulated in DCM mice compared with that in healthy control, suggesting that the environmental cue in heart failure might suppress the Gr-1(+) cells function. Activin A was upregulated in the serum of DCM models and induced downregulation of GH levels in Gr-1(+) cells and serum. Furthermore, humoral factors upregulated in heart failure including angiotensin II upregulated activin A in peripheral blood mononuclear cells (PBMNC) via activation of NFΞΊB. Similarly, serum activin A levels were also significantly higher in DCM patients with heart failure than in healthy subjects and the GH levels in conditioned medium from PBMNC of DCM patients were lower than that in healthy subjects. Inhibition of activin A increased serum GH levels and improved cardiac function of DCM model mice. These results suggest that activin A causes heart failure by suppressing GH activity and that inhibition of activin A might become a novel strategy for the treatment of heart failure
Extracellular High Mobility Group Box 1 Plays a Role in the Effect of Bone Marrow Mononuclear Cell Transplantation for Heart Failure
Transplantation of unfractionated bone marrow mononuclear cells (BMCs) repairs and/or regenerates the damaged myocardium allegedly due to secretion from surviving BMCs (paracrine effect). However, donor cell survival after transplantation is known to be markedly poor. This discrepancy led us to hypothesize that dead donor BMCs might also contribute to the therapeutic benefits from BMC transplantation. High mobility group box 1 (HMGB1) is a nuclear protein that stabilizes nucleosomes, and also acts as a multi-functional cytokine when released from damaged cells. We thus studied the role of extracellular HMGB1 in the effect of BMC transplantation for heart failure. Four weeks after coronary artery ligation in female rats, syngeneic male BMCs (or PBS only as control) were intramyocardially injected with/without anti-HMGB1 antibody or control IgG. One hour after injection, ELISA showed that circulating extracellular HMGB1 levels were elevated after BMC transplantation compared to the PBS injection. Quantitative donor cell survival assessed by PCR for male-specific sry gene at days 3 and 28 was similarly poor. Echocardiography and catheterization showed enhanced cardiac function after BMC transplantation compared to PBS injection at day 28, while this effect was abolished by antibody-neutralization of HMGB1. BMC transplantation reduced post-infarction fibrosis, improved neovascularization, and increased proliferation, while all these effects in repairing the failing myocardium were eliminated by HMGB1-inhibition. Furthermore, BMC transplantation drove the macrophage polarization towards alternatively-activated, anti-inflammatory M2 macrophages in the heart at day 3, while this was abolished by HMGB1-inhibition. Quantitative RT-PCR showed that BMC transplantation upregulated expression of an anti-inflammatory cytokine IL-10 in the heart at day 3 compared to PBS injection. In contrast, neutralizing HMGB1 by antibody-treatment suppressed this anti-inflammatory expression. These data suggest that extracellular HMGB1 contributes to the effect of BMC transplantation to recover the damaged myocardium by favorably modulating innate immunity in heart failure
GMP-conformant on-site manufacturing of a CD133+ stem cell product for cardiovascular regeneration
Significant improvement of direct reprogramming efficacy of fibroblasts into progenitor endothelial cells by ETV2 and hypoxia
Effect of neuronβderived neurotrophic factor on rejuvenation of human adiposeβderived stem cells for cardiac repair after myocardial infarction
Validating intramyocardial bone marrow stem cell therapy in combination with coronary artery bypass grafting, the PERFECT Phase III randomized multicenter trial: study protocol for a randomized controlled trial
<p>Abstract</p> <p>Background</p> <p>For the last decade continuous efforts have been made to translate regenerative cell therapy protocols in the cardiovascular field from βbench to bedsideβ. Successful clinical introduction, supporting safety, and feasibility of this new therapeutic approach, led to the initiation of the German, Phase III, multicenter trial - termed the PERFECT trial (ClinicalTrials.gov Identifier: NCT00950274), in order to evaluate the efficacy of surgical cardiac cell therapy on left ventricular function.</p> <p>Methods/Design</p> <p>The PERFECT trial has been designed as a prospective, randomized, double-blind, placebo controlled, multicenter trial, analyzing the effect of intramyocardial CD 133<sup>+</sup> bone marrow stem cell injection in combination with coronary artery bypass grafting on postoperative left ventricular function. The trial includes patients aged between 18 and 79βyears presenting with a coronary disease with indication for surgical revascularization and reduced global left ventricular ejection fraction as assessed by cardiac magnet resonance imaging. The included patients are treated in the chronic phase of ischemic cardiomyopathy after previous myocardial infarction.</p> <p>Discussion</p> <p>Patients undergoing coronary artery bypass grafting in combination with intramyocardial CD133<sup>+</sup> cell injection will have a higher LV ejection fraction than patient who undergo CABG alone, measured 6βmonths after the operation.</p> <p>Trial registration</p> <p>ClinicalTrials.gov Identifier: NCT00950274</p