Removal of some reactive dyes by untreated and pretreated <i>Saccharomyces cerevisiae, </i>an alcohol fermentation waste

632-639This study presents Saccharomyces cerevisiae, an alcohol fermentation waste, for removal of acidic dyes (Red 3:1 & Orange 13) even after chemical and physical modification. Biosorption performance was found 96.29% through untreated biomass and 97.31% through pretreated biomass with hydrogen peroxide solution for Red 3:1, whereas 93.49% through untreated biomass and 94.71% through pretreated biomass with dimethyl sulfoxide and phosphoric acid solution for Orange 13. Good results were obtained when modified biomass was used to treat dye wastewater. FTIR analyses, before and after treatment, suggest that increase in dye adsorption was due to hydrolysis of yeast. Besides, Freundlich and Langmuir adsorption models were found suitable for biosorptions of both dyestuffs

Phenol tolerance and biodegradation optimization of Serratia marcescens NSO9-1 using Plackett-Burman and Box-Behnken design

The phenol degradation capacity of Serratia marcescens NSO9-1 isolated from olive mill wastewater was evaluated and optimized in this study. Plackett-Burman design coupled with Box-Behnken methodology was used to evaluate the effects of medium components and significant parameters on phenol degradation by this relevant strain. According to Plackett-Burman-based statistical screening, seven of the eleven components of the medium had a significant effect on the metabolism of phenol degradation. The most important factors were MgSO4, NaCl, CaCh2 and molybdenum salt, which had an effective contribution of 90.12%. Additionally, Box-Behnken methodology using a quadratic model was adopted to investigate the mutual interactions between process parameters. The analysis results indicated that interactions between pH and temperature, pH and inoculum amount, and incubation time and inoculum amount critically affected the response variable. The experimental results showed that under the determined conditions, 41.66% of the maximum removal efficiency of phenol was achieved. The optimal conditions were 8.94, 30.22°C, 4.19 days, and 4.68% (v/v) for pH, temperature, incubation time, and inoculum amount, respectively. The validity and practicability of this statistical optimization strategy confirmed the relation between predicted and experimental values. Using a selective isolation method, the performance of this indigenous strain isolated from olive oil mill wastewater, which contained polyphenolic compounds, is comparable to the reported literature at higher phenol concentrations. © 2017 Gh. Asachi Technical University of Iasi. All right reserved

PHENOL TOLERANCE AND BIODEGRADATION OPTIMIZATION OF Serratia marcescens

The phenol degradation capacity of Serratia marcescens NSO9-1 isolated from olive mill wastewater was evaluated and optimized in this study. Plackett-Burman design coupled with Box-Behnken methodology was used to evaluate the effects of medium components and significant parameters on phenol degradation by this relevant strain. According to Plackett-Burmanbased statistical screening, seven of the eleven components of the medium had a significant effect on the metabolism of phenol degradation. The most important factors were MgSO4, NaCl, CaCl2, and molybdenum salt, which had an effective contribution of 90.12%. Additionally, Box-Behnken methodology using a quadratic model was adopted to investigate the mutual interactions between process parameters. The analysis results indicated that interactions between pH and temperature, pH and inoculum amount, and incubation time and inoculum amount critically affected the response variable. The experimental results showed that under the determined conditions, 41.66% of the maximum removal efficiency of phenol was achieved. The optimal conditions were 8.94, 30.22 degrees C, 4.19 days, and 4.68% (v/v) for pH, temperature, incubation time, and inoculum amount, respectively. The validity and practicability of this statistical optimization strategy confirmed the relation between predicted and experimental values. Using a selective isolation method, the performance of this indigenous strain isolated from olive oil mill wastewater, which contained polyphenolic compounds, is comparable to the reported literature at higher phenol concentrations

PHENOL TOLERANCE AND BIODEGRADATION OPTIMIZATION OF Serratia marcescens

The phenol degradation capacity of Serratia marcescens NSO9-1 isolated from olive mill wastewater was evaluated and optimized in this study. Plackett-Burman design coupled with Box-Behnken methodology was used to evaluate the effects of medium components and significant parameters on phenol degradation by this relevant strain. According to Plackett-Burmanbased statistical screening, seven of the eleven components of the medium had a significant effect on the metabolism of phenol degradation. The most important factors were MgSO4, NaCl, CaCl2, and molybdenum salt, which had an effective contribution of 90.12%. Additionally, Box-Behnken methodology using a quadratic model was adopted to investigate the mutual interactions between process parameters. The analysis results indicated that interactions between pH and temperature, pH and inoculum amount, and incubation time and inoculum amount critically affected the response variable. The experimental results showed that under the determined conditions, 41.66% of the maximum removal efficiency of phenol was achieved. The optimal conditions were 8.94, 30.22 degrees C, 4.19 days, and 4.68% (v/v) for pH, temperature, incubation time, and inoculum amount, respectively. The validity and practicability of this statistical optimization strategy confirmed the relation between predicted and experimental values. Using a selective isolation method, the performance of this indigenous strain isolated from olive oil mill wastewater, which contained polyphenolic compounds, is comparable to the reported literature at higher phenol concentrations