Genetic differentiation between two geographic subpopulations of Bermeya goat

Ponencia publicada en ITEA, vol.104Este trabajo es un análisis preliminar de la diversidad y grado de diferenciación genética entre las subpoblaciones Oriental y Occidental de la cabra de raza Bermeya de Asturias. Se han genotipado 27 microsatélites en 122 individuos pertenecientes a las poblaciones de cabra Bermeya Oriental, Bermeya Occidental, del Guadarrama, Alpine y Saanen. Las poblaciones de raza Bermeya presentaron heterocigosis esperadas menores de 0,6 y coascendencias moleculares dentro de población de 0,419, reflejando una alta identidad genética entre individuos. Asimismo, el número medio de alelos por locus, ajustado por el tamaño muestral, de las poblaciones Bermeya Oriental y Occidental fueron los menores encontrados (3,8 y 3,9, respectivamente). La mayor coascendencia molecular se encontró entre las poblaciones Bermeya Oriental-Guadarrama (0,388 ± 0,006), como consecuencia de la introgresión, en el Oriente asturiano de individuos de tipo Pirenáico. Este parámetro entre las poblaciones de Bermeya Oriental y Occidental fue de 0,371 ± 0,007. Puede ser necesario establecer estrategias de conservación diferenciadas para las dos poblaciones geográficas de cabra Bermeya.Two geographic subpopulations (Eastern and Western) of the Asturian Bermeya goat were analysed using 27 microsatellites. A total of 122 individuals of Bermeya, Guadarrama, Alpine and Saanen goat breeds were genotyped. The Eastern and Western Bermeya subpopulations had expected heterozygosity values lower than 0.6 and within-subpopulations molecular coancestry values of 0.419, thus illustrating a high between individuals genetic identity. The rarefacted average number of alleles per locus found in both the Eastern and the Western Bermeya subpopulations were the lowest in the dataset (3.8 and 3.9, respectively). The higher between-populations molecular coancestry values was found for the pair Eastern Bermeya-Guadarrama (0.388 ± 0.006), thus reflecting the introgression of the Pyrenean-type goat into Eastern Asturias. This parameter between the two Bermeya subpopulations was of 0.371 ± 0.007. It could be recommended the implementation of different conservation strategies for each of the geographic subpopulations of Bermeya goat

Characterization of the Oral Microbiome of Medicated Type-2 Diabetes Patients

Type 2 diabetes mellitus (T2DM) is a chronic metabolic disease that is becoming a significant global health care problem. Several studies have shown that people with diabetes are more susceptible to oral problems, such as periodontitis and, although the causes are still inconclusive, oral microbiota is considered to play a major role in oral health. This study aimed to characterize the oral microbiome of a sample representing T2DM patients from Portugal and exploit potential associations between some microorganisms and variables like teeth brushing, smoking habits, average blood sugar levels, medication and nutrient intake. By sequencing the hypervariable regions V3-V4 of the 16S rRNA gene in 50 individuals belonging to a group of diabetes patients and a control group, we found a total of 232 taxa, from which only 65% were shared between both groups. No differences were found in terms of alpha and beta diversity between categories. We did not find significant differences in the oral microbiome profiles of control and diabetes patients. Only the class Synergistia and the genus TG5, which are related to periodontitis, were statistically more frequent in the control group. The similar microbiome profiles of medicated diabetics and the control group indicates that the relationship between the T2DM and the oral microbiome might be more related to either the lifestyle/diet rather than diabetes per se. Moreover, this study provides, for the first time, insights into the oral microbiome of a population with a high prevalence of diabetes.This work was supported by the European Union’s Horizon 2020 Research and Innovation Programme under the Grant Agreement Number 857251. LP-P was funded by national funds from FCT—Fundação para a Ciência e a Tecnologia, I.P. MG-V was funded through UID/BIA/50027/2019 from FCT

Allelic and genotypic frequencies of ASIP and MC1R genes in four West African sheep populations

In West Africa, consumers pay a major attention on the coat colour of the sheep due to religious and cultural reasons. White coated individuals reach selling prices up to three-fold higher than black coated sheep. The aim of this study was to ascertain the genotypic and allelic frequencies of MC1R and ASIP genes in order to assess possible implementation of breeding programmes focusing on the increase of the white coated sheep frequencies. A total of 113 individuals belonging to three Burkina Faso sheep breeds (Burkina-Sahel, Djallonké and Mossi) and one Niger sheep breed (Touareg) were genotyped for the MC1R and ASIP genes. The wild allele of the ASIP gene (Awt; 54.30%) was the most frequent in the four West African sheep, particularly in Burkina-Sahel (85%) and Touareg breeds (80%). The dominant black ED allele was not identified in Burkina-Sahel and Touareg. Most of the analysed individuals were homozygous for wild MC1R allele (E+/E+) with 100, 73.5, 59 and 100% frequency in Burkina-Sahel, Djallonké, Mossi and Touareg, respectively. The Awt/Awt was the most frequent genotype on the ASIP gene in the four West African breeds (80.53%). No individuals were homozygous for the deletion (allele Adel). Although, no routine methods for detection of the genetic basis of the recessive black coat colour patterns can be easily implemented, the current results suggest the feasibility of a selection programme aiming at decreasing the frequencies of the dominant black ED allele in Burkina Faso sheep breeds.Key words: Sheep, coat colour, MC1R gene, ASIP gene, Burkina Faso, Niger