Unraveling the Stratification of an Iron-Oxidizing Microbial Mat by Metatranscriptomics

International audienceA metatranscriptomic approach was used to study community gene expression in a naturally occurring iron-rich microbial mat. Total microbial community RNA was reversely transcribed and sequenced by pyrosequencing. Characterization of expressed gene sequences provided accurate and detailed information of the composition of the transcriptionally active community and revealed phylogenetic and functional stratifications within the mat. Comparison of 16S rRNA reads and delineation of OTUs showed significantly lower values of metatranscriptomic-based richness and diversity in the upper parts of the mat than in the deeper regions. Taxonomic affiliation of rRNA sequences and mRNA genome recruitments indicated that iron-oxidizing bacteria affiliated to the genus Leptothrix, dominated the community in the upper layers of the mat. Surprisingly, type I methanotrophs contributed to the majority of the sequences in the deep layers of the mat. Analysis of mRNA expression patterns showed that genes encoding the three subunits of the particulate methane monooxygenase (pmoCAB) were the most highly expressed in our dataset. These results provide strong hints that iron-oxidation and methane-oxidation occur simultaneously in microbial mats and that both groups of microorganisms are major players in the functioning of this ecosystem

Convention sur les droits de l'homme et la Biomédecine

International audienceHector GROS ESPIEL, Jean MICHAUD, Gérard TEBOUL (avec la coordination de Laurence AZOUX BACRIE),Analyses et commentaires, 674 pages, 2010, Economic

Le droit de la bioéthique

International audienceStéphanie HENNETTE-VAUCHEZ,Coll. Repères, Ed. La Découverte, 126 pages, 200

Cures thermales. Accidents du travail et maladies professionnelles. Prise en charge

International audience(Circ. DSS/AT n° 98-397 du 1er juillet 1998, BO min. Solidarité et Santé, n° 98/30, 8 août 1998, p. 219

Dépistage des maladies graves. Assurance maladie. Prise en charge

International audience(Décr. n° 200-495 du 2 juin 2000, JO 7 juin 2000

Assurances sociales. Prestations. Frais de transport. Ambulance. Remboursement. Prescription médicale

International audience(Soc. 31 mars 1994

Accès aux soins. Lutte contre les exclusions

International audience(L. n° 98-657 du 29 juill. 1998, JO 31 juill. 1998

Sécurité sociale. Assurance-maladie. Convention. Organisations représentatives des professions médicales

International audience(Arr. du 28 mars 1997, JO 29 mars 1997, p. 4897 et 4922

Assurance maladie. Soins dispensés aux détenus en milieu hospitalier. Financement

International audience(Circ. DH/SDAF n° 94-30 du 24 août 1994 relative au financement des soins dispensés aux détenus en milieu hospitalier. BOMASSV n° 94-33 du 6 oct. 1994

La médecine libérale : quel avenir ?

International audienceNuméro hors série RDSS 2011, 141 page