19 research outputs found
Acute ethanol exposure increases the susceptibility of the donor hearts to ischemia/reperfusion injury after transplantation in rats
An altered pattern of myocardial histopathological and molecular changes underlies the different characteristics of type-1 and type-2 diabetic cardiac dysfunction
Pharmacological activation of soluble guanylate cyclase activator protects the heart against isoproterenol-induced myocardial ischemia in rats
Az 1-es és 2-es tÃpusú diabéteszes kardiális diszfunkció hátterében álló eltérÅ‘ miokardiális szövettani és molekuláris jellegzetességek = An altered pattern of myocardial histopathological and molecular changes underlies the different characteristics of type-1 and type-2 diabetic cardiac dysfunction
Cinaciguat prevents neointima formation after arterial injury by decreasing vascular smooth muscle cell migration and proliferation
Nitric oxide- and heme-independent activation of soluble guanylate cyclase attenuates peroxynitrite-induced endothelial dysfunction in rat aorta
Acute ethanol exposure increases the susceptibility of the donor hearts to ischemia/reperfusion injury after transplantation in rats.
BACKGROUND: Many donor organs come from youths involved in alcohol-related accidental death. The use of cardiac allografts for transplantation from donors after acute poisoning is still under discussion while acute ethanol intoxication is associated with myocardial functional and morphological changes. The aims of this work were 1) to evaluate in rats the time-course cardiac effects of acute ethanol-exposure and 2) to explore how its abuse by donors might affect recipients in cardiac pump function after transplantation. METHODS: Rats received saline or ethanol (3.45 g/kg, ip). We evaluated both the mechanical and electrical aspects of cardiac function 1 h, 6 h or 24 h after injection. Plasma cardiac troponin-T and glucose-levels were measured and histological examination of the myocardium was performed. In addition, heart transplantation was performed, in which donors received ethanol 6 h or 24 h prior to explantation. Graft function was measured 1 h or 24 h after transplantation. Myocardial TBARS-concentration was measured; mRNA and protein expression was assessed by quantitative real-time PCR and Western blot, respectively. RESULTS: Ethanol administration resulted in decreased load-dependent (-34 ± 9%) and load-independent (-33 ± 12%) contractility parameters, LV end-diastolic pressure and elevated blood glucose levels at 1 h, which were reversed to the level of controls after 6 h and 24 h. In contrast to systolic dysfunction, active relaxation and passive stiffness are slowly recovered or sustained during 24 h. Moreover, troponin-T-levels were increased at 1 h, 6 h and 24 h after ethanol injection. ST-segment elevation (+47 ± 10%), elongated QT-interval (+38 ± 4%), enlarged cardiomyocyte, DNA-strand breaks, increased both mRNA and protein levels of superoxide dismutase-1, glutathione peroxydase-4, cytochrome-c-oxidase and metalloproteinase-9 were observed 24 h following ethanol-exposure. After heart transplantation, decreased myocardial contractility and relaxation, oxidative stress and altered protein expression were observed. CONCLUSIONS: These results demonstrate acute alcohol abuse increases the susceptibility of donor hearts to ischemia/reperfusion in a rat heart transplant model even though the global contractile function recovers 6 h after ethanol-administration
Effect of acute ethanol exposure on cardiac function.
<p>Assessment of (A) load-dependent (dP/dt<sub>max</sub>) and (B) load-independent (dP/dt<sub>max</sub>-EDV) contractility parameters revealed a significant decline only at 1 h in response to acute ethanol when compared with the control group. Whereas (C) maximal slope of the diastolic pressure decrement (dP/dt<sub>min</sub>) 1 h and 6 h after ethanol administration was significantly decreased, (D) end-diastolic pressure-volume relationship (EDPVR) was significantly increased in rats treated with ethanol after 1 h, 6 h and 24 h. Eth indicates ethanol. *p<0.05 versus control.</p
Effect of ethanol exposure on graft Troponin I expression 1 h and 24 h after heart transplantation.
<p>(<b>A</b>) 1 h and (B) 24 h after transplantation, graft Troponin I expression was significantly increased in the groups receiving ethanol 6 h or 24 h prior to explantation when compared with the control group. I/R indicates ischemia/reperfusion, Eth ethanol *p<0.05 versus I/R groups.</p
Effect of acute ethanol exposure on myocardial protein expression.
<p>Densitometric analysis of bands for (A) superoxide dismutase (SOD)-1 showed a significant increase after 6 h and 24 h ethanol administration, and (B) glutathione peroxidase (GPx)-4, (C) cytochrome-c oxidase (cyto-c oxi) and (D) MMP-9 following 24 h, compared with the control group. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Eth indicates ethanol. *p<0.05 versus control; <sup>#</sup>p<0.05 versus ethanol 1 h, <sup>$</sup>p<0.05 versus ethanol 6 h.</p