Effects of chronic REMsleep deprivation on lipocalin-2, nitric oxide synthase-3, interleukin-6 and cardiotrophin-1 levels: an experimental rat model

The purpose of this study was to investigate the effects of chronic rapid eye movement sleep deprivation on cardiovascular system using blood levels of lipocalin-2, nitric oxide synthase-3, interleukin-6, and cardiotrophin-1. We included sixteen Wistar Albino rats of 300-350g weight in the study. To create a chronic rapid eye movement sleep deprivation, we used water tanks with platform including modified multiple sleep deprivation platforms, and we used water tanks with grid to grab for control group. We left 8 rats in water tanks with platform (study group) and 8 rats in water tanks with grid to grab (control group) for 21 days. Finally, we drew blood from the hearts of the rats, just before scarifying all the rats. We performed statistical comparisons of lipocalin-2, nitric oxide synthase-3, interleukin-6 and cardiotrophin-1 levels between the study group and the control group. The lipocalin-2, nitric oxide synthase-3, interleukin-6 and cardiotrophin-1 levels were 29.8ng/mL, 116.99ng/mL, 4.32ng/L and 33.26ng/L in the study group, respectively; and 122.74ng/mL, 85.74ng/mL, 3.23ng/L and 23.02ng/L in the control group, respectively. In the study group, mean lipocalin-2 levels were significantly lower (p=0.003) but mean cardiotrophin-1 levels were significantly higher compared with the control group (p=0.001). Mean interleukin-6 and nitric oxide synthase-3 levels did not significantly differ between the groups (p=0.135 and p=0.157). Our study showed that chronic rapid eye movement sleep deprivation might be associated with cardiovascular diseases owing to higher blood cardiotropin-1 levels in the study group, a novel biomarker that has been shown to indicate endothelial dysfunction, atherosclerosis and fibrosis in the cardiovascular system

Effect of Disulfide Bonds on the Thermal Stability of Pediocin: In-silico Screening Using Molecular Dynamics Simulation

The thermal stability properties of pediocin at 310, 313, 323, 333, 343, and 348 K (37, 40, 50, 60, 70, and 75°C, respectively) are reported in this study. A theoretical approach, such as the molecular dynamics method, was used to analyze the structure. Molecular dynamics simulation confirms the stability of molecules with Cys. Furthermore, this study reveals that Cys residues play an essential role in structure stability at high temperatures. To understand the structural basis for the stability of pediocin, a detailed in-silico analysis using molecular dynamics simulations to explore the thermal stability profiles of the compounds was conducted. This study shows that thermal effects fundamentally alter the functionally crucial secondary structure of pediocin. However, as previously reported, pediocin’s activity was strictly conserved due to the disulfide bond between Cys residues. These findings reveal, for the first time, the dominant factor behind the thermodynamic stability of pediocin

Chronic Demyelinating Polyradiculoneuropathy Associated with "Human Immunodeficiency Virus" Infection: Different Clinical Features and Diagnostic Problems

During the course of human immunodeficiency virus (HIV) infection, which leads to a chronic infectious disease that suppresses the cellular immune system, various types of peripheral neuropathies can be seen in different stages from seroconversion to the advanced stage. Chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) usually occurs in early or mid-stage of the disease. In this study, we are presenting a 66 year-old man who was admitted to our clinic due to progressive and predominantly sensorial symptoms and signs of CIDP, who had recently been operated for carpal tunnel syndrome which was suggested by electromyography even though he had complains of diffuse polyneuropathy symptoms from the beginning. During his diagnostic work-up cerebrospinal fluid (CSF) analysis had revealed elevated protein and pleocytosis, and further investigation revealed HIV-1 infection. Areas of demyelination are very common in usual entrapment zones in the course of CIDP, which is a neuropathy with multi-segment demyelination in peripheral nerves and/nerve roots. Without a detailed patient history and examination, and a detailed EMG evaluation, a diffuse neuropathy can be misdiagnosed as an entrapment neuropathy. As it is in our case, this can lead to an unnecessary surgery and a delay in the actual diagnosis. CSF examination is very valuable for the investigation of the etiology of chronic demyelinating polyneuropaties. In CIDP patients, only protein elevation can be seen in CSF analysis; however, in the presence of pleocytosis, other diseases such as chronic infections, particularly HIV infection, other chronic inflammatory diseases, and malignant infiltration of nerve roots should be investigated

Chronic Demyelinating Polyradiculoneuropathy Associated with "Human Immunodeficiency Virus" Infection: Different Clinical Features and Diagnostic Problems

WOS: 000335224200025During the course of human immunodeficiency virus (HIV) infection, which leads to a chronic infectious disease that suppresses the cellular immune system, various types of peripheral neuropathies can be seen in different stages from seroconversion to the advanced stage. Chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) usually occurs in early or mid-stage of the disease. In this study, we are presenting a 66 year-old man who was admitted to our clinic due to progressive and predominantly sensorial symptoms and signs of CIDP, who had recently been operated for carpal tunnel syndrome which was suggested by electromyography even though he had complains of diffuse polyneuropathy symptoms from the beginning. During his diagnostic work-up cerebrospinal fluid (CSF) analysis had revealed elevated protein and pleocytosis, and further investigation revealed HIV-1 infection. Areas of demyelination are very common in usual entrapment zones in the course of CIDP, which is a neuropathy with multi-segment demyelination in peripheral nerves and/nerve roots. Without a detailed patient history and examination, and a detailed EMG evaluation, a diffuse neuropathy can be misdiagnosed as an entrapment neuropathy. As it is in our case, this can lead to an unnecessary surgery and a delay in the actual diagnosis. CSF examination is very valuable for the investigation of the etiology of chronic demyelinating polyneuropaties. In CIDP patients, only protein elevation can be seen in CSF analysis; however, in the presence of pleocytosis, other diseases such as chronic infections, particularly HIV infection, other chronic inflammatory diseases, and malignant infiltration of nerve roots should be investigated

The assessment of malnutrition and severity of gastrointestinal disease by using symptom-based questionnaires in systemic sclerosis: is it related to severe organ involvement or capillary rarefaction at microcirculation?

Objective. Gastrointestinal (GI) system is commonly affected in sytemic sclerosis (SSc) patients who are also known to be at risk for malnutrition. We aimed to investigate the relationship between severity of GI disease, malnutrition and severity of organ involvement including microvasculopathy

The Potential Role of STAT's in Anti-Leukemic Therapy with Different Drugs

54th Annual Meeting and Exposition of the American-Society-of-Hematology (ASH) -- DEC 08-11, 2012 -- Atlanta, GAWOS: 000314049605219Amer Soc Hematol (ASH