Combinations of Interleukin-10 gene promoter polymorphisms with -1082A, -819T, -592A minor allele are associated with sinonasal polyposis

S inonasal polyposis SP is an inflammatory disease involving multiple etiologies and pathogenesis. The disarray of Interleukin IL -10 is associated with a raised immunopathological response during the progression of many autoimmune diseases as well as the response to infection. We studied the possible role of the single nucleotide polymorphisms SNPs in IL-10 gene and their genotypic combinations in the SP pathogenesis. The IL-10’s promoter was genotyped in 200 participants 100 patients and 100 controls . The sites that were encompassed -1082, -819, and -592 SNP regions of extracted DNAs were analyzed by sequencing for polymorphisms. The IL-10 gene promoter polymorphisms with -1082A, -819T, -592A minor alleles, their heterozygotes and homozygotes mutant genotypes hadsigniŞcantly higher risks for SP

Effect of glucagon-like peptide-1(7-36) and exendin-4 on the vascular reactivity in streptozotocin/nicotinamide-induced diabetic rats

We investigated the vascular effects of glucagon-like peptide-1 (GLP-1) and Exendin-4 in type 2 diabetic rat aortae. Studies were performed in a normal control group (NC) (0.2 ml i. p. saline, n = 10), streptozotocin (STZ)/ nicotinamide diabetic control group ( DC) ( a single dose of 80 mg/kg STZ i. p. injection 15 min after administration of 230 mg/kg nicotinamide i. p.), GLP-1 (GLPC) control group ( 1 mu g/kg twice daily i. p. for 1 month, n = 10), Exendin-4 control group (EXC) ( 0.1 mu g/kg twice daily i. p. for 1 month, n = 10), GLP-1-treated diabetic group (GLPT) ( 1 mu g/kg twice daily i. p. for 1 month, n = 10), and Exendin-4-treated diabetic group (EXT) ( 0.1 mu g/kg twice daily i. p. for 1 month, n = 10). One month of GLP-1 and Exendin-4 treatment significantly decreased the blood glucose levels of diabetic rats ( 113 +/- 2 mg/dl, p < 0.001, and 117 +/- 1 mg/ dl, p < 0.001, respectively versus 181 +/- 9 mg/ dl in the DC group). Sensitivity (pD(2)) and maximum response (% Max. Relax) of acetylcholine-stimulated relaxations in the DC group (pD(2) : 6.73 +/- 0.12 and 55 +/- 6, respectively) were decreased compared with the nondiabetic NC group (pD(2) : 7.41 +/- 0.25, p < 0.05, and 87 +/- 4, p < 0.01). Treating diabetic rats with GLP-1, pD(2) values and with Exendin-4, Max. Relax % values of aortic strips to acetylcholine returned to near non-diabetic NC values (pD(2) : 7.47 +/- 0.15, p < 0.05, and 87 +/- 3, p < 0.01, respectively). Maximal contractile responses (E-max) to noradrenaline in aortic strips from the diabetic DC group (341 +/- 27 mg tension/mg wet weight) were significantly decreased compared with the non-diabetic NC ( 540 +/- 66 mg tension/mg wet weight, p < 0.001) and the GLPT group ( 490 +/- 25 mg tension/ mg wet weight, p < 0.05). There were no significant differences in pD(2) values of aortic strips to noradrenaline from all groups. E-max to KCl in aortic strips from the DC group ( 247 +/- 10 mg tension/ mg wet weight, p < 0.01) was significantly decreased compared with non-diabetic NC group ( 327 +/- 26 mg tension/ mg wet weight). Treating diabetic rats with GLP-1 (GLPT), E-max values of aortic strips to KCl returned to near non-diabetic NC values ( 271 +/- 12 mg tension/ mg wet weight). GLP-1 and ( partially) Exendin-4 treatment could improve the increased blood glucose level and normalize the altered vascular tone in type 2 diabetic rats. Copyright (C) 2005 S. Karger AG, Basel

Evaluation Of Autoinflammatory Disease Genes In Nasal Polyposis

Background/aim: To investigate cold-induced autoinflammatory syndrome 1 (CIAS1) gene polymorphisms that cause autoinflammatory diseases in patients with nasal polyposis (NP). Materials and methods: The study included 30 patients diagnosed with NP and 30 healthy age-matched individuals as a control group. CIAS1 polymorphisms were assessed by DNA sequence analysis. Patients with nasal polyps and the control group were compared in terms of gene polymorphisms. Each of the 8 polymorphisms of the CIAS1 gene was analyzed separately in the patient group. Results: The most frequently observed polymorphisms in the patient group were c.732G > A in 83%, c.663C > T in 23%, and c.1308C > A in 23% of the patients. c.732G > A polymorphism was evaluated separately. Guanine was transformed to adenine at the 732nd nucleotide position of the CIAS1 gene in the cDNA of chromosome 1. Conclusion: The CIAS1 gene c.732G > A polymorphism was thought to be responsible for an increase in disease susceptibility. The frequency of the "A" allele is higher in the patient group compared to the control group. Autoinflammatory diseases seem like a candidate to be one of these factors. This is the first report to define the role of autoinflammatory diseases among these factors.WoSScopu

Maternal Serum Amyloid A Levels In Pregnancies Complicated With Preterm Prelabour Rupture Of Membranes

Objective: The aim of the study was to investigate a possible association between maternal serum amyloid A levels (SAA) and maternal and fetal parameters in pregnancies complicated with preterm prelabor rupture of membranes (PPROM). Material and methods: A total of 88 pregnant women (PPROM group, n=44 and control group, n=44) were included into this prospective case control study. Serum blood samples for SAA were obtained from both groups within 1h since the rupture of the membranes and before administration of any medicine. The samples were kept frozen at -70 degrees C until the analysis. The recorded risk factors were: age, gravidity, parity, delivery mode, gender, fetal birth weight, APGAR scores, white blood cell count, microCRP, neutrophil/lymphocyte ratio (NLR), and maternal serum SAA levels. Results: Demographic characteristics showed no statistically significant differences between the groups (p>0.05). The mode of delivery mode was cesarean section: 41% and 43.2% in the study and the control group, respectively, and this difference was statistically significant between the groups (p<0.05). Fetal parameters also showed statistically significant differences (p<0.05). There was a statistically significant difference between the groups in terms of micro CRP, NLR and SAA. SAA levels were higher in the PPROM group (p<0.005). SAA levels at a cut-off 95.63 ng/ml. Conclusion: We are of the opinion that second trimester maternal serum SAA level may be a predictive marker for PPROM. However, further studies with more participants are required.WoSScopu