Determination of the effect of fluconazole against Candida albicans and Candida glabrata by using microbroth kinetic assay

Aim: To evaluate the antifungal activity of a model antifungal (fluconazole) against Candida albicans (ATCC 90028) and Candida glabrata (RSKK 04019) strains using a microbroth kinetic assay based on continuous monitoring of changes in the optical density of fungal growth. Recently, antifungal susceptibility testing has become more important because of the increasing incidence of both fungal infections and antifungal drug resistance

Serum malondialdehyde level in children infected with Vampirolepis nana

Background: Vampirolepis nana is the only human tapeworm in which the intermediate host is not necessary and transmission is from person to person. In this study the changes of serum malondialdehyde level, that is, the oxidative stress hypothesis in patients infected with V. nana, was investigated

Synthesis and antioxidant and antimicrobial evaluation of novel 4-substituted-1H-1,2,4-triazole derivatives

A series of 4-benzyl/phenyl-3-(1-methyl-1H-indole-2-yl)-1H-1,2,4-triazole-5(4H)-thione (4a,b) and 2-{4-[benzyl/phenyl-5-(substitutedbenzylthio)]-4H-1,2,4-triazole-3-yl}-1-methyl-1H-indole derivatives (5a-p) were synthesized and evaluated for their in vitro scavenging of DPPH and superoxide radical, and lipid peroxidation inhibition effects as well as their antimicrobial properties. DPPH radical scavenging capacity was found to be equipotent with BHT and found in compounds containing 1,2,4-triazole-5(4H) -thione moiety (4a,b). With regard to antimicrobial properties, compound 5k showed slight antimicrobial activity against all the test microorganisms

Serum malondialdehyde levels in patients infected with Plasmodium vivax

Malondialdehyde, a lipid peroxide, may be used as an indicator of oxidative stress. This study investigated the oxidative stress hypothesis in patients infected with Plasmodium vivax. Serum malondialdehyde (MDA) concentration activity was measured in 87 patients. Scores were obtained for the patients and 60 seronegative healthy controls matched for age and gender The difference between MDA levels of patients infected with Plasmodium vivax and the control group was statistically significant both for females (p 0.05) in both females and males. In addition, no significant correlation could be found between MDA levels of both females and males for patients and control group (p > 0.05). MDA levels were increased in the patients infected with Plasmodium vivax

Serum malondialdehyde level in patients infected with Ascaris lumbricoides

AIM: The aim of the study was to investigate the changes of serum malondialdehyde level, i.e; the oxidative stress hypothesis in patients infected with Ascaris lumbricoides

Anti- leishmanial activities of ethanolic extract of Kayseri propolis

Propolis is a natural product which honey bees collect from plants then mix it with their wax. The present study has involved the investigation of antileishmanial effect of propolis against the Leishmania tropica parasite. In this study, propolis that was used was collected from Kayseri, a city in the Middle Anatolian Region of Turkey. An ethanolic extract of propolis was obtained. The propolis concentrations varying from 0.0625 mu g/ml to 1024 mu g/ml were prepared using serial dilutions. These propolis concentrations were added on parasites. The parasites were examined daily under an inverted microscope at the 24(th), 48(th), 72(nd) and 96(th) h. Mobility, morphology, and density were reported. It has been observed with microscopic examination that propolis inhibited parasites beginning at 32 mu g/ml concentration. Also, it was determined that the antileishmanial effects of propolis increased with increasing concentrations and incubation periods. It has been shown that Kayseri propolis, which is a natural product, was effective against Leishmania tropica, even in low concentrations

In vitro antimicrobial activity of propolis, BioPure MTAD, sodium hypochlorite, and chlorhexidine on Enterococcus faecalis and Candida albicans

Objectives: To evaluate the antimicrobial effect by measuring the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of propolis, BioPure MTAD, 5% sodium hypochlorite (NaOCl), and 2% chlorhexidine (CHX) on Enterococcus faecalis (E. faecalis) and Candida albicans (C. albicans) in vitro.OBJECTIVE:To evaluate the antimicrobial effect by measuring the minimum inhibitory concentration MIC and minimum bactericidal concentration MBC of propolis, BioPure MTAD, 5% sodium hypochlorite (NaOCl), and 2% chlorhexidine CHX on Enterococcus faecalis (E. faecalis) and Candida albicans (C. albicans) in vitro.METHODS:This study was performed in the Faculty of Dentistry and Pharmacy at Erciyes University, Kayseri, Turkey from February to April 2010. Ethanol extract of propolis (EEP) was prepared from propolis collected from Kayseri, Turkey, and proper media for microorganisms were prepared using sterile broth medium to give final concentrations between 0.002-2.4 mg/ml for propolis, 0.000125-0.512 mg/ml for CHX, and 1:2-1:4096 dilutions for NaOCl and BioPure MTAD. Using the macrobroth dilution method, MIC, and MBC values of irrigants on the growth of E. faecalis and C. albicans were determined.RESULTS:Propolis and other irrigants were found to be effective on C. albicans and E. faecalis. Propolis and NaOCl were more effective in lower concentrations on C. albicans than on E. faecalis. In contrast, CHX and MTAD were more effective in lower concentrations on E. faecalis than on C. albicans.CONCLUSION:Propolis showed antimicrobial activity against E. faecalis and C. albicans. It appears that propolis is an effective intracanal irrigant in eradicating E. faecalis and C. albicans.</p