219 research outputs found
Behavior in dogs with spontaneous hypothyroidism during treatment with levothyroxine
Background Thyroid hormone supplementation anecdotally has been described as a valid treatment option for dogs with aggressionârelated problems. However, prospective, controlled, and blinded trials evaluating behavior and neurohormonal status in hypothyroid dogs during treatment with levothyroxine are lacking. Objective Levothyroxine supplementation will have a significant influence on the behavior and neurohormonal status of dogs with spontaneous hypothyroidism. Animals Twenty clientâowned dogs diagnosed with spontaneous hypothyroidism. Methods This prospective study was to evaluate the behavior of dogs, which was screened at initial presentation, and after 6âweeks, and 6âmonths of treatment with levothyroxine (starting dosage 10âÎŒg/kg PO q12h) using the standardized Canine Behavioral Assessment and Research Questionnaire (CâBARQ). At each time period, circulating serotonin and prolactin (PRL) concentrations were evaluated using a commercially validated ELISA kit and heterologous radioimmunoassay, respectively. Results After 6âweeks of thyroid hormone supplementation, CâBARQ scores demonstrated a significant increase in activity of hypothyroid dogs (Pââ.99 and Pâ=â.46) and PRL (Pâ=â.99 and Pâ=â.37) were noted between the 6âweek and 6âmonth periods compared with baseline. Conclusions and Clinical Importance The results of this study indicate increased activity of hypothyroid dogs after 6âweeks of thyroid hormone supplementation. None of the hypothyroid dogs in this cohort showed a significant change in any of the evaluated behavioral signs and neurohormonal status after 6âmonths of thyroid hormone supplementation
Investigation of Focused Cardiac Ultrasound in the Emergency Room for Differentiation of Cardiac and Non-Cardiac Causes of Respiratory Distress in Dogs
Structure of an archaeal PCNA1-PCNA2-FEN1 complex: elucidating PCNA subunit and client enzyme specificity.
The archaeal/eukaryotic proliferating cell nuclear antigen (PCNA) toroidal clamp interacts with a host of DNA modifying enzymes, providing a stable anchorage and enhancing their respective processivities. Given the broad range of enzymes with which PCNA has been shown to interact, relatively little is known about the mode of assembly of functionally meaningful combinations of enzymes on the PCNA clamp. We have determined the X-ray crystal structure of the Sulfolobus solfataricus PCNA1-PCNA2 heterodimer, bound to a single copy of the flap endonuclease FEN1 at 2.9 A resolution. We demonstrate the specificity of interaction of the PCNA subunits to form the PCNA1-PCNA2-PCNA3 heterotrimer, as well as providing a rationale for the specific interaction of the C-terminal PIP-box motif of FEN1 for the PCNA1 subunit. The structure explains the specificity of the individual archaeal PCNA subunits for selected repair enzyme 'clients', and provides insights into the co-ordinated assembly of sequential enzymatic steps in PCNA-scaffolded DNA repair cascades
1.3 mm Wavelength VLBI of Sagittarius A*: Detection of Time-Variable Emission on Event Horizon Scales
Sagittarius A*, the ~4 x 10^6 solar mass black hole candidate at the Galactic
Center, can be studied on Schwarzschild radius scales with (sub)millimeter
wavelength Very Long Baseline Interferometry (VLBI). We report on 1.3 mm
wavelength observations of Sgr A* using a VLBI array consisting of the JCMT on
Mauna Kea, the ARO/SMT on Mt. Graham in Arizona, and two telescopes of the
CARMA array at Cedar Flat in California. Both Sgr A* and the quasar calibrator
1924-292 were observed over three consecutive nights, and both sources were
clearly detected on all baselines. For the first time, we are able to extract
1.3 mm VLBI interferometer phase information on Sgr A* through measurement of
closure phase on the triangle of baselines. On the third night of observing,
the correlated flux density of Sgr A* on all VLBI baselines increased relative
to the first two nights, providing strong evidence for time-variable change on
scales of a few Schwarzschild radii. These results suggest that future VLBI
observations with greater sensitivity and additional baselines will play a
valuable role in determining the structure of emission near the event horizon
of Sgr A*.Comment: 8 pages, submitted to ApJ
Microbiomes attached to fresh perennial ryegrass are temporally resilient and adapt to changing ecological niches
Abstract Background Gut microbiomes, such as the rumen, greatly influence host nutrition due to their feed energy-harvesting capacity. We investigated temporal ecological interactions facilitating energy harvesting at the fresh perennial ryegrass (PRG)-biofilm interface in the rumen using an in sacco approach and prokaryotic metatranscriptomic profiling. Results Network analysis identified two distinct sub-microbiomes primarily representing primary (†4 h) and secondary (â„ 4 h) colonisation phases and the most transcriptionally active bacterial families (i.e Fibrobacteriaceae, Selemondaceae and Methanobacteriaceae) did not interact with either sub-microbiome, indicating non-cooperative behaviour. Conversely, Prevotellaceae had most transcriptional activity within the primary sub-microbiome (focussed on protein metabolism) and Lachnospiraceae within the secondary sub-microbiome (focussed on carbohydrate degradation). Putative keystone taxa, with low transcriptional activity, were identified within both sub-microbiomes, highlighting the important synergistic role of minor bacterial families; however, we hypothesise that they may be âcheatingâ in order to capitalise on the energy-harvesting capacity of other microbes. In terms of chemical cues underlying transition from primary to secondary colonisation phases, we suggest that AI-2-based quorum sensing plays a role, based on LuxS gene expression data, coupled with changes in PRG chemistry. Conclusions In summary, we show that fresh PRG-attached prokaryotes are resilient and adapt quickly to changing niches. This study provides the first major insight into the complex temporal ecological interactions occurring at the plant-biofilm interface within the rumen. The study also provides valuable insights into potential plant breeding strategies for development of the utopian plant, allowing optimal sustainable production of ruminants. Video Abstrac
Correction to: Microbiomes attached to fresh perennial ryegrass are temporally resilient and adapt to changing ecological niches
Following the publication of the original article [1], it was noticed that the figure image of Fig. 6 should be for Fig. 3. The image for Fig. 3 should be for Fig. 5 and Fig. 6 was missing. The correct Fig. 6 have been provided below and the original article has been updated to correct Figs. 3, 5 and 6. (Figure presented.)
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