The use of reproductive vigor descriptors in studying genetic variability in nine Tunisian faba bean (Vicia faba L.) populations

A collection of nine Tunisian faba bean (Vicia faba) populations belonging to three botanical classes (Var. minor, var. equina and var. major) was evaluated using twenty seven agro-morphological traits. Analysis of variance, correlation coefficients and principal components analysis (PCA) were performed based on MVSP 3.13 program. Significant differences between populations were noted for most agromorphological traits in four main groups. The first group, positively correlated to the two axes, isrepresented by ‘Bachaar’ belonging to V. faba. var. minor, the second group, including V. faba. var. minor population (‘Massri’ and ‘Badï’), is positively correlated to the PC1 and negatively correlated to the PC2 while the third group, is composed of two V. faba var. major (‘Malti’ and ‘Batata’) and were positively and negatively correlated to the PC2 and PC1, respectively. Finally, the fourth group negatively correlated to the two axes, gathers the remaining population (‘Chahbi’, ‘Chemlali’, ‘Aguadulce’ and ‘Super Aguadulce’). The dendrogram based on Nei's genetic distance of the 9 populations using UPGMA method, show some genetic drift between populations.Key words: Faba bean, agromorphological traits, principal components analysis, UPGMA method

Generating inner ear organoids containing putative cochlear hair cells from human pluripotent stem cells

In view of the prevalence of sensorineural hearing defects in an ageing population, the development of protocols to generate cochlear hair cells and their associated sensory neurons as tools to further our understanding of inner ear development are highly desirable. We report herein a robust protocol for the generation of both vestibular and cochlear hair cells from human pluripotent stem cells which represents an advance over currently available methods that have been reported to generate vestibular hair cells only. Generating otic organoids from human pluripotent stem cells using a three-dimensional culture system, we show formation of both types of sensory hair cells bearing stereociliary bundles with active mechano-sensory ion channels. These cells share many morphological characteristics with their in vivo counterparts during embryonic development of the cochlear and vestibular organs and moreover demonstrate electrophysiological activity detected through single-cell patch clamping. Collectively these data represent an advance in our ability to generate cells of an otic lineage and will be useful for building models of the sensory regions of the cochlea and vestibule

Yield and Yield Components of Faba Bean (Vicia faba L.) as Influenced by Supplemental Irrigation under Semi-arid Region of Tunisia

Abstract A field experiment was conducted at the research station of Higher Agriculture School of Kef located in a semi-arid region of Tunisia to study the effect of supplemental irrigation on yield and yield components of four Tunisian faba bean genotypes (Bachaar, Badii, Chahbi and locale). Two supplemental irrigations were applied at the flowering and pod formation stages. Results showed a significant effect of supplemental irrigation on biological yield (BY/P), seed number per plant (SN/P), 100-seed weight (100 SW), grain yield (GY/m 2 ), harvest index (HI) and number of days to maturity (NDM). Grain yields under supplemental irrigation varied from 83.9 to 208.7 g/m2, and they varied from 18.6 to 65.8 g/m 2 under drought conditions. Average 100-seeds weight increment due to supplemental irrigation condition was 52.8%. Results showed also that under rain fed condition, Bachar genotypes required minimum number of days to maturity (134.3 days). Drought susceptibility index (DSI) values for grain yield ranged from 0.8 to 1.13. Chahbi was relatively drought resistant (DSI values <1). This genotype proved high yielding and drought tolerant and can be incorporated in stress breeding programme for the development of drought tolerant faba bean varieties

3D Deformable Super-Resolution for Multi-Camera 3D Face Scanning

International audienceLow-cost and high-accuracy 3D face measurementis becoming increasingly important in many computer visionapplications including face recognition, facial animation,games, orthodontics and aesthetic surgery. In most casesfringe projection based systems are used to overcome therelatively uniform appearance of skin. These systems employa structured light camera/projector device and requireexplicit user cooperation and controlled lighting conditions.In this paper, we propose a 3D acquisition solution with a3D space-time non-rigid super-resolution capability, usingthree calibrated cameras coupled with a non calibrated projectordevice, which is particularly suited to 3D face scanning,i.e. rapid, easily movable and robust to ambient lightingvariation. The proposed solution is a hybrid stereovisionand phase-shifting approach, using two shifted patterns anda texture image, which not only takes advantage of stereovisionand structured light, but also overcomes their weaknesses.The super-resolution scheme involves a shape+texture3D non-rigid registration for 3D artifacts correction in thepresence of small non-rigid deformations as facial expressions