A Matrix Formulation of the Least-Squares Method and its Application to Nuclear Decay Schemes

Many people call physics an \u27\u27exact science . This may be true depending on our definition of exact. Most dictionaries include as a definition of exact the words strictly accurate . The average man thinks of an exact science as one which yields strictly accurate\u27\u27 results. Physics then is exact only to a degree

Culinary School for Healthy Eating: Healthy Choices Without Sacrificing Taste

An ounce of prevention is worth a pound of cure. Food is a very important aspect that can make a big difference in our lives yet is very often neglected. The main research area of this project will be designing a culinary school that keeps healthy living and a balance life as its primary objective. This will be seen not only in culinary instruction but the environment that they cook in as well. The site location for this project is in Bismarck North Dakota along the Missouri River. This project will be using partial adaptive use as there is already a deserted hotel conference building on the site. History will also play a role in the design of this culinary school. Over the years food preparation has changed dramatically. Researching the history of culinary development and how it has changed, will lead to an understanding of how food will influence architecture

The Transformative Power of the 2030 U.N. Sustainable Development Goals

West Central Initiative, a mostly rural community foundation and regional development organization in Minnesota, integrated the United Nations 17 Sustainable Development Goals into its strategic plan in 2019. This article explores how aligning the U.N. goals with the foundation’s “nested strategy” of local, regional, and global goals has aligned and energized the disparate functions of the organization. This article describes the strategic planning process that led to adoption of the goals, articulates how they have helped evolve the interplay of economic development and philanthropy, and identifies lessons learned from the first two years of working with the goals. Focusing on the strong and undeniable connections between the local and the global has crystalized West Central Initiative’s higher purpose. The new, transformative vision for the foundation centers diversity, equity, and inclusion as essential building blocks of both successful regional development and place-based philanthropy. Any region — anywhere — with a successful regional economy that also is supported by effective community philanthropy would look like the Sustainable Development Goals, realized

Down, Set, Like? A Study of Social Networking and Sports Fandom

Sports in the 21st century have become popular across multiple industries, and a major boon to a television industry dealing with increasing audience fragmentation. So an understanding of fans' behavior is important to all parties. This study, an online survey consisting of 242 responses, examined fandom and its relationship with time spent using social networking sites and found no statistical correlation. Six uses and gratification factors obtained: human connection, network content, distraction/amusement, social integration, social surveillance, and active entertainment. The low comparative saliency of the social integration factor suggests that perhaps fandom is distinct from other ways of identifying with similarly-minded individuals (e.g. political and/or religious affiliation), or that perhaps fandom as a factor is less than sufficient to explain how/why sports fans use social networking sites

Zinc-finger domains of the transcriptional repressor KLF15 bind multiple sites in rhodopsin and IRBP promoters including the CRS-1 and G-rich repressor elements

BACKGROUND: In the retina, many of the genes that encode components of the visual transduction cascade and retinoid recycling are exclusively expressed in photoreceptor cells and show highly stereotyped temporal and spatial expression patterns. Multiple transcriptional activators of photoreceptor-specific genes have been identified, but little is known about negative regulation of gene expression in the retina. We recently identified KLF15, a member of the Sp/Krüppel-like Factor family of zinc-finger containing transcription factors, as an in vitro repressor of the promoters of the photoreceptor-specific genes rhodopsin and IRBP/Rbp3. To gain further insight into the mechanism of KLF15-mediated regulation of gene expression, we have characterized the binding characteristics and specificity of KLF15's DNA binding domains and defined the KLF15 binding sites in the rhodopsin and IRBP promoters. RESULTS: In EMSA and DNAseI footprinting assays, a KLF15-GST fusion protein containing the C-terminal zinc-finger domains (123 amino acids) showed zinc-dependent and sequence-specific binding to a 9 bp consensus sequence containing a core CG/TCCCC. Both the bovine rhodopsin and IRBP promoters contained multiple KLF15 binding sites that included the previously identified CRS-1 and G-rich repressor elements. KLF15 binding sites were highly conserved between the bovine, human, chimp and dog rhodopsin promoters, but less conserved in rodents. KLF15 reduced luciferase expression by bRho130-luc (containing 4 KLF15 sites) and repressed promoter activation by CRX (cone rod homeobox) and/or NRL (neural retina leucine zipper), although the magnitude of the reduction was smaller than previously reported for a longer bRho225-luc (containing 6 KFL15 sites). CONCLUSION: KLF15 binds to multiple 9 bp consensus sites in the Rhodospin and IRBP promoters including the CRS-1 and G-rich repressor elements. Based on the known expression pattern of KLF15 in non-photoreceptor cells, we hypothesize an in vivo role for KLF15 in repressing photoreceptor-specific gene expression in the inner retina

Class I histone deacetylases in retinal progenitors and differentiating ganglion cells

Background The acetylation state of histones has been used as an indicator of the developmental state of progenitor and differentiating cells. The goal of this study was to determine the nuclear localization patterns of Class I histone deacetylases (HDACs) in retinal progenitor cells (RPCs) and retinal ganglion cells (RGCs), as the first step in understanding their potential importance in cell fate determination within the murine retina. Results The only HDAC to label RPC nuclei at E16 and P5 was HDAC1. In contrast, there was generally increased nuclear localization of all Class I HDACs in differentiating RGCs. Between P5 and P30, SOX2 expression becomes restricted to Müller glial, cholinergic amacrine cells, and retinal astrocytes. Cholinergic amacrine showed a combination of changes in nuclear localization of Class I HDACs. Strikingly, although Müller glia and retinal astrocytes express many of the same genes, P30 Müller glial cells showed nuclear localization only of HDAC1, while retinal astrocytes were positive for HDACs 1, 2, and 3. Conclusion These results indicate there may be a role for one or more of the Class I HDACs in retinal cell type-specific differentiation

Histone deacetylase expression patterns in developing murine optic nerve

BACKGROUND: Histone deacetylases (HDACs) play important roles in glial cell development and in disease states within multiple regions of the central nervous system. However, little is known about HDAC expression or function within the optic nerve. As a first step in understanding the role of HDACs in optic nerve, this study examines the spatio-temporal expression patterns of methylated histone 3 (K9), acetylated histone 3 (K18), and HDACs 1–6 and 8–11 in the developing murine optic nerve head. RESULTS: Using RT-qPCR, western blot and immunofluorescence, three stages were analyzed: embryonic day 16 (E16), when astrocyte precursors are found in the optic stalk, postnatal day 5 (P5), when immature astrocytes and oligodendrocytes are found throughout the optic nerve, and P30, when optic nerve astrocytes and oligodendrocytes are mature. Acetylated and methylated histone H3 immunoreactivity was co-localized in the nuclei of most SOX2 positive glia within the optic nerve head and adjacent optic nerve at all developmental stages. HDACs 1–11 were expressed in the optic nerve glial cells at all three stages of optic nerve development in the mouse, but showed temporal differences in overall levels and subcellular localization. HDACs 1 and 2 were predominantly nuclear throughout optic nerve development and glial cell maturation. HDACs 3, 5, 6, 8, and 11 were predominantly cytoplasmic, but showed nuclear localization in at least one stage of optic nerve development. HDACs 4, 9 and10 were predominantly cytoplasmic, with little to no nuclear expression at any time during the developmental stages examined. CONCLUSIONS: Our results showing that HDACs 1, 2, 3, 5, 6, 8, and 11 were each localized to the nuclei of SOX2 positive glia at some stages of optic nerve development and maturation and extend previous reports of HDAC expression in the aging optic nerve. These HDACs are candidates for further research to understand how chromatin remodeling through acetylation, deacetylation and methylation contributes to glial development as well as their injury response