12 research outputs found
Assessing the genetic diversity of 48 groundnut (Arachis hypogaea L.) genotypes in the Guinea savanna agro-ecology of Ghana, using microsatellite-based markers
Groundnut (Arachis hypogaea L.) is the most important grain legume in Ghana. However, its production
is constrained by a myriad of biotic and abiotic stresses which necessitate the development and use of
superior varieties for increased yield. Germplasm characterisation both at the phenotypic and molecular
level is important in all plant breeding programs. The aim of this study was to characterise selected
advanced breeding groundnut lines with different phenotypic attributes at the molecular level using
simple sequence repeats (SSR) markers in Ghana. A total of 53 SSR markers were screened and 25 were
found to be polymorphic with an average polymorphic information content (PIC) value of 0.57. Of the 48
groundnut genotypes studied, 67% showed very close relationship (~100% similarity) with one or more
genotypes among themselves. In fact, there were 14 instances where two to three genotypes within the
same sub-cluster exhibited 100% similarity even though they displayed different phenotypic attributes.
The remaining 33% of the groundnut genotypes were distant from each other and could therefore serve
as effective parental material for future work. In this study, the SSR-based markers were found to be
quite discriminatory in discerning variations between and among groundnut lines even where the level
of variation was low. Microsatellite-based markers therefore represent a useful tool for dissecting
genetic variations in cultivated crops, especially groundnu
Genetic diversity and population structure of groundnut (Arachis hypogaea L.) accessions using phenotypic traits and SSR markers: implications for rust resistance breeding
Groundnut (Arachis hypogaea L.) is a
multi-purpose legume serving millions of farmers and
their value chain actors globally. Use of old poorperforming
cultivars contributes to low yields (\1
t/ha) of groundnut in sub-Saharan Africa including
Tanzania. The objectives of this study were to
determine the extent of genetic variation among
diverse groundnut collections using phenotypic traits
and simple sequence repeat (SSR) markers to select
distinct and complementary genotypes for breedin
Marketâled options to scale up legume seeds in developing countries: Experiences from the Tropical Legumes Project
There are several hurdles to ensure sustainable seed production and consistent flow of improved legume varieties in subâSaharan Africa (SSA) and South Asia (SA). The unreliable demand, autogamous nature of most of the grain legumes, and slow variety replacement rate by smallholder farmers do not provide strong incentive for private seed companies to invest in legume seed business. Unless a well thoughtâout and comprehensive approach to legume seed delivery is developed, current seed shortages will continue, eroding emerging market opportunities. The experiences reported here are collated through a 10âyear partnership project, the Tropical Legumes in SSA and SA. It fostered innovative publicâprivate partnerships in joint testing of innovative marketâled seed systems, skills and knowledge enhancement, deârisking private sector initiatives that introduced in new approaches and previously overlooked entities in technology delivery. As new public and private seed companies, individual seed entrepreneurs and farmer organizations emerged, the existing ones enhanced their capacities. This resulted in significant rise in production, availability and accessibility of various seed grades of newly improved and farmer demanded legume varieties in the target countries
Genetic diversity and population structure of groundnut (Arachis hypogaea L.) accessions using phenotypic traits and SSR markers: implications for rust resistance breeding
Groundnut (Arachis hypogaea L.) is a multi-purpose legume serving millions of farmers and their value chain actors globally. Use of old poor-performing cultivars contributes to low yields (<â1 t/ha) of groundnut in sub-Saharan Africa including Tanzania. The objectives of this study were to determine the extent of genetic variation among diverse groundnut collections using phenotypic traits and simple sequence repeat (SSR) markers to select distinct and complementary genotypes for breeding. One hundred and nineteen genotypes were evaluated under field conditions for agronomic traits and susceptibility to rust and leaf spot diseases. The study was conducted in two locations across two seasons. In addition, the 119 accessions were profiled with 13 selected SSR markers. Genotype and genotype by environment interaction effects were significant (pâ<â0.05) for days to flowering (DTF), late leaf spot score at 85 and 100 days after planting, pod yield (PDY), kernel yield (KY), hundred seed weight (HSW) and shelling percentage (SP). Principal components analysis revealed that plant stand, KY, SP, NPP (number of pods per plant), late leaf spot and rust disease scores accounted for the largest proportion of the total variation (71.9%) among the tested genotypes. Genotypes ICGV-SM 08587 and ICGV-SM 16579 had the most stable yields across the test environments. Moderate genetic variation was recorded with mean polymorphic information content of 0.34 and gene diversity of 0.63 using the SSR markers. The majority (74%) of genotypes showed high membership coefficients to their respective sub-populations, while 26% were admixtures after structure analysis. Much of the variation (69%) was found within populations due to genotypic differences. The present study identified genotypes ICGV-SM 06737, ICGV-SM 16575, ICG 12725 and ICGV-SM 16608 to be used for development of mapping population, which will be useful for groundnut improvement. This study provided a baseline information on characterization and selection of a large sample of groundnut genotypes in Tanzania for effective breeding and systematic conservation
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QUALITY OF SEED LOTS OF SOYBEAN [Glycine max (L.) MERRILL] GENOTYPES PRODUCED IN A GUINEA SAVANNA AGROECOLOGY OF GHANA
Crop yield is a derivative of the number of plants harvested. In Ghana, soybean fields are mostly sparsely populated with less than the optimum number of plants, a phenomenon that contributes to poor yields of only 46% of the cropâs potential in farmersâ fields. The low plant population could be the result of the poor quality of the seed planted. Meanwhile, the quality of seed is determined by crop production practices, the environmental conditions of the mother plant, and postharvest handling technologies used. Over 90% of the countryâs soybean seed comes from the northern part of Ghana, predominantly the Guinea Savannah. In this study, seed lots of soybean genotypes produced within the Savanna Agroecological Zone of Ghana, were evaluated for physical and physiological traits, and the prevalence of seed borne fungi. Results showed that seed lots produced within the zone and stored under ambient conditions have medium-sized (100 to 140 g/1000 seeds) seeds with an average seed weight of 133 g/1000 seeds. The seed lots evaluated had physical purity values higher than the minimum certification standard of 98% for soybean seed in Ghana. Wrinkled seeds were the most dominant component of the seed with visual defects followed closely by seeds that were discoloured, cracked, and those with purple stains. Aspergillus flavus, Aspergillus niger, Chaetomium globosum, Rhizopus sp and Macrophomina phaseolina were the main fungi identified. Furthermore, the seed lots had an average germination percentage of 86%, which is within the acceptable minimum certification standards for soybean seed in Ghana. Though germination and vigour were variable among the seed lots tested, these parameters were not affected by the presence of the fungi species identified and the amount of seed with visual defects. The findings of this study strongly suggest that the low plant density and the resultant poor yields of soybean usually recorded in farmersâ fields in Ghana are likely due to suboptimal seeding rates at planting coupled with the low use of good agronomic practices with only a small fraction attributable to poor seed quality
Genetic diversity, population structure and key phenotypic traits driving variation within soyabean (Glycine max) collection in Ghana
Published online: 30 April 2019Soybean [Glycine max (L.) Merrill] is an important oilseed crop worldwide and it has recently become the crop of interest in Ghana. In this study, 142 soybean accessions were genotyped with 34 SSR markers and concurrently evaluated for five quantitative and two qualitative phenotypic traits. Twentyânine of the SSR markers were polymorphic with mean allele number of 5.3, polymorphic information content (PIC) of 0.51 and gene diversity of 0.55. Molecular analysis based on unweighted paired group arithmetic mean (UPGMA) clustering and principal coordinate analysis (PCoA) was similar in explaining the extent of diversity within the accessions. Structure analysis placed most of the accessions into two subpopulations with 18 (12.7%) as admixtures. Principal component analysis (PCA) based on phenotypic traits revealed two clusters. Both UPGMA clusteringâbased SSR data and PCA from phenotypic data showed similar results. The assembled germplasm is genetically diverse with high variation in flowering and maturity period, and key yield components which could be exploited in developing superior varieties well adapted to Ghana and West Africa
Advances in groundnut breeding for drought prone West and Central Africa
The West and Central Africa region accounts for more than 70% of the groundnut production in Africa. The crop is challenged by various biotic and abiotic production constraints with drought being the main abiotic constraint. ICRISAT has been working with national breeding programs to develop improved groundnut varieties. The approach used included identifying sources of resistance, developing populations, evaluating in target environments and releasing improved farmer preferred varieties for production. The Tropical Legumes project initiated in 2007 has been instrumental in strengthening the breeding program in the region. Nine drought resistant/tolerant accessions were identified from evaluation of the mini core collections, and these accessions have been utilized as parents in crossing programs. Since 2007, ICRISAT distributed more than 1000 advanced breeding lines to national programs. Farmer participatory variety selection was found very useful for fast track release and adoption of improved varieties. Twenty two varieties have been released/registered across the region as a result of project efforts (4 in Ghana, 5 in Mali, 4 in Niger, 3 in Nigeria and 6 in Senegal). These varieties are high yielding (yield advantage of >20%) with resistance or tolerance to drought and major diseases. Currently, efforts are underway to improve the efficiency of breeding programs and to enhance genetic gain. These include designing product pipelines based on traits of breeding interest; reducing generation advancement process of breeding populations by growing 2 to 3 generations per year; integrating modern genomic tools; digitizing data collection, analysis, management and sharing by using BMS; and enhancing the skills of breeders and technicians
Public confidence in the police: tests of instrumental and expressive models in a developing country city
Genomics, genetics and breeding of tropical legumes for better livelihoods of smallholder farmers
Legumes are important components of sustainable agricultural production, food, nutrition and income systems of developing countries. In spite of their importance, legume crop production is challenged by a number of biotic (diseases and pests) and abiotic stresses (heat, frost, drought and salinity), edaphic factors (associated with soil nutrient deficits) and policy issues (where less emphasis is put on legumes compared to priority starchy staples). Significant research and development work have been done in the past decade on important grain legumes through collaborative bilateral and multilateral projects as well as the CGIAR Research Program on Grain Legumes (CRPâGL). Through these initiatives, genomic resources and genomic tools such as draft genome sequence, resequencing data, largeâscale genomewide markers, dense genetic maps, quantitative trait loci (QTLs) and diagnostic markers have been developed for further use in multiple genetic and breeding applications. Also, these megaâinitiatives facilitated release of a number of new varieties and also dissemination of onâtheâshelf varieties to the farmers. More efforts are needed to enhance genetic gains by reducing the time required in cultivar development through integration of genomicsâassisted breeding approaches and rapid generation advancement