15 research outputs found
Π‘Π΅ΠΏΡΠΈΡ-Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½Π°Ρ ΡΠ½ΡΠ΅ΡΠ°Π»ΠΎΠΏΠ°ΡΠΈΡ (ΠΎΠ±Π·ΠΎΡ)
International studies demonstrate an annual increase in the frequency and impact of sepsis in intensive care units (ICU). Cerebral dysfunction, or so-called sepsis-associated encephalopathy (SAE), is one of the earliest signs of sepsis, as well as its common complication. Up to 70% of patients with sepsis have symptoms of encephalopathy [1, 2]. A direct link between the SAE and an increased mortality rate is a major concern; more than a half of sepsis survivors experience continuous memory and concentration impairment [3β5]. Diagnosis of the cerebral dysfunction in sepsis is often difficult because of lack of specific biomarkers and frequent prescription of sedatives to critically ill patients. Clinical manifestations of SAE are diverse, may vary from simple malaise and lack of concentration to deep coma. The literature discusses probable mechanisms of formation and development of septic encephalopathy, such as oxidative stress, inflammation, mitochondrial and endothelial dysfunction, increased permeability of the blood-brain barrier, impairment of macro- and microcirculation, changes in neirotransmission, activation of microglia. The lack of clear data and consensus about the etiology and mechanisms of SAE at present does not permit predicting its development and prescribing a specific therapy. The review discusses current understanding of the causes of septic encephalopathy, methods of diagnosis, the main clinical manifestations, key mediators, and pathophysiological mechanisms. A hypothesis is proposed that poses a contribution of aromatic microbial metabolites (AMM) of phenol and indole nature (products of bacterial biodegradation of tyrosine and tryptophan) to the development of brain dysfunction. The fields of exploratory studies, which might open perspectives in the diagnosis, as well as new approaches in the treatment of SAE are outlined.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΌΠ΅ΠΆΠ΄ΡΠ½Π°ΡΠΎΠ΄Π½ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΡΠΊΠ°Π·ΡΠ²Π°ΡΡ Π½Π° Π΅ΠΆΠ΅Π³ΠΎΠ΄Π½ΡΠΉ ΡΠΎΡΡ ΡΠ°ΡΡΠΎΡΡ ΠΈ Π°ΠΊΡΡΠ°Π»ΡΠ½ΠΎΡΡΠΈ ΡΠ΅ΠΏΡΠΈΡΠ° Π² ΡΠ΅Π°Π½ΠΈΠΌΠ°ΡΠΈΠΎΠ½Π½ΡΡ
ΠΎΡΠ΄Π΅Π»Π΅Π½ΠΈΡΡ
. ΠΠ΄Π½ΠΈΠΌ ΠΈΠ· Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΠ°Π½Π½ΠΈΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ², Π° ΡΠ°ΠΊΠΆΠ΅ ΡΠ°ΡΡΡΠΌ ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠ΅ΠΌ ΡΠ΅ΠΏΡΠΈΡΠ° ΡΠ²Π»ΡΠ΅ΡΡΡ Π΄ΠΈΡΡΡΠ½ΠΊΡΠΈΡ ΠΌΠΎΠ·Π³Π° ΠΈΠ»ΠΈ ΡΠ°ΠΊ Π½Π°Π·ΡΠ²Π°Π΅ΠΌΠ°Ρ ΡΠ΅ΠΏΡΠΈΡ-Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½Π°Ρ ΡΠ½ΡΠ΅ΡΠ°Π»ΠΎΠΏΠ°ΡΠΈΡ (SAE). ΠΠΎ 70% Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ ΡΠ΅ΠΏΡΠΈΡΠΎΠΌ ΠΈΠΌΠ΅ΡΡ ΡΠΈΠΌΠΏΡΠΎΠΌΡ ΡΠ½ΡΠ΅ΡΠ°Π»ΠΎΠΏΠ°ΡΠΈΠΈ [1, 2]. ΠΡΠΎΠ±ΠΎΠ΅ Π±Π΅ΡΠΏΠΎΠΊΠΎΠΉΡΡΠ²ΠΎ Π²ΡΠ·ΡΠ²Π°Π΅Ρ ΡΠ°ΠΊΡ ΠΏΡΡΠΌΠΎΠΉ ΡΠ²ΡΠ·ΠΈ SAE Ρ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΠΎΠΉ Π»Π΅ΡΠ°Π»ΡΠ½ΠΎΡΡΡΡ, Π° ΡΡΠ΅Π΄ΠΈ Π²ΡΠΆΠΈΠ²ΡΠΈΡ
ΠΏΠΎΡΠ»Π΅ ΡΠ΅ΠΏΡΠΈΡΠ° Π±ΠΎΠ»Π΅Π΅ ΠΏΠΎΠ»ΠΎΠ²ΠΈΠ½Ρ ΠΈΠΌΠ΅ΡΡ Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΡΠ΅ ΠΊΠΎΠ³Π½ΠΈΡΠΈΠ²Π½ΡΠ΅ ΡΠ°ΡΡΡΡΠΎΠΉΡΡΠ²Π°, Π½Π°ΡΡΡΠ΅Π½ΠΈΡ ΠΏΠ°ΠΌΡΡΠΈ ΠΈ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ Π²Π½ΠΈΠΌΠ°Π½ΠΈΡ [3β5]. ΠΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ° Π΄ΠΈΡΡΡΠ½ΠΊΡΠΈΠΈ ΠΌΠΎΠ·Π³Π° ΠΏΡΠΈ ΡΠ΅ΠΏΡΠΈΡΠ΅ ΡΠ°ΡΡΠΎ Π·Π°ΡΡΡΠ΄Π½Π΅Π½Π° ΠΈΠ·-Π·Π° ΠΎΡΡΡΡΡΡΠ²ΠΈΡ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ
Π±ΠΈΠΎΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ², Π° ΡΠ°ΠΊΠΆΠ΅ Π² ΡΠ²ΡΠ·ΠΈ Ρ ΡΠ°ΡΡΡΠΌ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΡΠ΅Π΄Π°ΡΠΈΠ²Π½ΡΡ
ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² Ρ ΠΊΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ
Π±ΠΎΠ»ΡΠ½ΡΡ
. ΠΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΡΠΎΡΠ²Π»Π΅Π½ΠΈΡ SAE ΡΠ°Π·Π½ΠΎΠΎΠ±ΡΠ°Π·Π½Ρ, ΠΌΠΎΠ³ΡΡ Π²Π°ΡΡΠΈΡΠΎΠ²Π°ΡΡ ΠΎΡ ΠΏΡΠΎΡΡΠΎΠ³ΠΎ Π½Π΅Π΄ΠΎΠΌΠΎΠ³Π°Π½ΠΈΡ ΠΈ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΊΠ° ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ Π²Π½ΠΈΠΌΠ°Π½ΠΈΡ Π΄ΠΎ Π³Π»ΡΠ±ΠΎΠΊΠΎΠΉ ΠΊΠΎΠΌΡ. Π Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΠ΅ ΠΎΠ±ΡΡΠΆΠ΄Π°ΡΡΡΡ ΠΏΡΠ΅Π΄ΠΏΠΎΠ»Π°Π³Π°Π΅ΠΌΡΠ΅ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΡ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΈ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΡΠ΅ΠΏΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ½ΡΠ΅ΡΠ°Π»ΠΎΠΏΠ°ΡΠΈΠΈ, ΡΠ°ΠΊΠΈΠ΅ ΠΊΠ°ΠΊ ΠΎΠΊΠΈΡΠ»ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ ΡΡΡΠ΅ΡΡ, Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΠ΅, ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½Π°Ρ ΠΈ ΡΠ½Π΄ΠΎΡΠ΅Π»ΠΈΠ°Π»ΡΠ½Π°Ρ Π΄ΠΈΡΡΡΠ½ΠΊΡΠΈΡ, ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΠΏΡΠΎΠ½ΠΈΡΠ°Π΅ΠΌΠΎΡΡΠΈ Π³Π΅ΠΌΠ°ΡΠΎΡΠ½ΡΠ΅ΡΠ°Π»ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π±Π°ΡΡΠ΅ΡΠ°, Π½Π°ΡΡΡΠ΅Π½ΠΈΡ ΠΌΠ°ΠΊΡΠΎ- ΠΈ ΠΌΠΈΠΊΡΠΎΡΠΈΡΠΊΡΠ»ΡΡΠΈΠΈ, ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Π½Π΅ΠΉΡΠΎΡΡΠ°Π½ΡΠΌΠΈΡΡΠΈΠΈ, Π°ΠΊΡΠΈΠ²Π°ΡΠΈΡ ΠΌΠΈΠΊΡΠΎΠ³Π»ΠΈΠΈ. ΠΡΡΡΡΡΡΠ²ΠΈΠ΅ ΡΠ΅ΡΠΊΠΈΡ
Π΄Π°Π½Π½ΡΡ
ΠΈ Π΅Π΄ΠΈΠ½ΠΎΠ³ΠΎ ΠΌΠ½Π΅Π½ΠΈΡ ΠΎ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΠΈ ΠΈ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΠ°Ρ
SAE Π² Π½Π°ΡΡΠΎΡΡΠ΅Π΅ Π²ΡΠ΅ΠΌΡ Π½Π΅ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΏΡΠΎΠ³Π½ΠΎΠ·ΠΈΡΠΎΠ²Π°ΡΡ Π΅Π΅ ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ ΠΈ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΡΡ ΡΠΏΠ΅ΡΠΈΠ°Π»ΡΠ½ΡΡ ΡΠ΅ΡΠ°ΠΏΠΈΡ. Π ΠΎΠ±Π·ΠΎΡΠ΅ ΠΎΠ±ΡΡΠΆΠ΄Π°ΡΡΡΡ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΠ΅ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ΠΈΡ ΠΎ ΠΏΡΠΈΡΠΈΠ½Π°Ρ
ΡΠ°Π·Π²ΠΈΡΠΈΡ ΡΠ΅ΠΏΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ½ΡΠ΅ΡΠ°Π»ΠΎΠΏΠ°ΡΠΈΠΈ, ΠΌΠ΅ΡΠΎΠ΄Π°Ρ
Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ, ΡΠ°ΡΡΠΌΠΎΡΡΠ΅Π½Ρ ΠΎΡΠ½ΠΎΠ²Π½ΡΠ΅ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΡΠΎΡΠ²Π»Π΅Π½ΠΈΡ, ΠΊΠ»ΡΡΠ΅Π²ΡΠ΅ ΠΌΠ΅Π΄ΠΈΠ°ΡΠΎΡΡ, ΠΏΠ°ΡΠΎΡΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΡ. ΠΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½Π° Π³ΠΈΠΏΠΎΡΠ΅Π·Π° ΠΎ ΡΠΎΠ»ΠΈ Π°ΡΠΎΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΌΠΈΠΊΡΠΎΠ±Π½ΡΡ
ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠΎΠ² (ΠΠΠ) ΡΠ΅Π½ΠΎΠ»ΡΠ½ΠΎΠΉ ΠΈ ΠΈΠ½Π΄ΠΎΠ»ΡΠ½ΠΎΠΉ ΠΏΡΠΈΡΠΎΠ΄Ρ β ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ² Π±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π±ΠΈΠΎΠ΄Π΅Π³ΡΠ°Π΄Π°ΡΠΈΠΈ ΡΠΈΡΠΎΠ·ΠΈΠ½Π° ΠΈ ΡΡΠΈΠΏΡΠΎΡΠ°Π½Π° β Π² ΡΠ°Π·Π²ΠΈΡΠΈΠΈ Π΄ΠΈΡΡΡΠ½ΠΊΡΠΈΠΈ ΠΌΠΎΠ·Π³Π°, Π½Π°ΠΌΠ΅ΡΠ΅Π½Ρ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½ΠΈΡ ΠΏΠΎΠΈΡΠΊΠΎΠ²ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ, ΠΎΡΠΊΡΡΠ²Π°ΡΡΠΈΠ΅ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Ρ Π² Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ΅ ΠΈ Π½ΠΎΠ²ΡΠ΅ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄Ρ Π² Π»Π΅ΡΠ΅Π½ΠΈΠΈ SAE
ΠΠ΅ΠΉΡΠΎΠΏΡΠΎΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ Ρ Π»ΠΎΡΠΈΠ΄Π° Π»ΠΈΡΠΈΡ Π½Π° ΠΌΠΎΠ΄Π΅Π»ΠΈ ΠΎΡΡΠ°Π½ΠΎΠ²ΠΊΠΈ ΡΠ΅ΡΠ΄ΡΠ° Ρ ΠΊΡΡΡ
Lithium chloride, which is used for the treatment of bipolar disorders, has a neuroprotective eο¬ect in conditions associated with acute and chronic circulatory disorders.The purpose of the study: to investigate the eο¬cacy of lithium chloride for the prevention of post-resuscitation death of hippocampal neurons during the post-resuscitation period.Material and methods. Cardiac arrest for 10 minutes was evoked in mature male rats by intrathoracic clumping of the vascular bundle of the heart, followed by resuscitation. 40 mg/kg or 20 mg/kg of 4,2% lithium chloride (LiCl) was injected intraperitoneally 1 hour before cardiac arrest, on the 1st and 2nd day after resuscitation (n=9). Untreated animals received equivalent doses of saline (n=9). Rats after a sham surgery served as a reference group (n=10). The number of viable neurons in the CA1 and CA3/CA4 ο¬elds of the hippocampus was estimated in slides stained with cresyl violet by day 6 or 7 postresuscitation. In a separate series of experiments, at the same terms, we studied the eο¬ect of lithium chloride on the protein content of GSK3Ξ² (glycogen synthase kinase) in brain tissue using Western-Blot analysis.Results.Β Histological assay showed that a 10-minute cardiac arrest resulted in a decrease in the number of viable neurons in the hippocampal CA1 ο¬eld β by 37.5% (P0.001), in the CA3/CA4 ο¬eld β by 12.9% (P0.05) vs. the reference group. Lithium treatment increased the number of viable neurons in resuscitated rats β in the CA1 ο¬eld by 37% (P<0.01), in the CA3/CA4 ο¬eld β by 11.5% (P0.1) vs. the untreated animals. It was found that lithium caused an increase in phosphorylated form of GSK3Ξ²: by 180% vs. the reference group (P[1]0.05), and by 150% vs. the untreated animals (P0.05).Conclusion. Lithium treatment leads to a pronounced neuroprotection in the neuronal populations of the hippocampus post-resuscitation. This eο¬ect may be due to an increase in the content of the phosphorylated form of GSK3Ξ² protein. The results indicate a high potential of lithium for the prevention and treatment of neurodegenerative disorders caused by a temporary arrest of blood circulation.Β Π₯Π»ΠΎΡΠΈΠ΄ Π»ΠΈΡΠΈΡ, ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΠ΅ΠΌΡΠΉ Π΄Π»Ρ ΠΊΠΎΡΡΠ΅ΠΊΡΠΈΠΈ Π±ΠΈΠΏΠΎΠ»ΡΡΠ½ΡΡ
ΡΠ°ΡΡΡΡΠΎΠΉΡΡΠ², ΠΎΠ±Π»Π°Π΄Π°Π΅Ρ Π½Π΅ΠΉΡΠΎΠΏΡΠΎΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌ ΡΡΡΠ΅ΠΊΡΠΎΠΌ ΠΏΡΠΈ ΡΠΎΡΡΠΎΡΠ½ΠΈΡΡ
, ΡΠ²ΡΠ·Π°Π½Π½ΡΡ
Ρ ΠΎΡΡΡΡΠΌ ΠΈ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΌ Π½Π°ΡΡΡΠ΅Π½ΠΈΠ΅ΠΌ ΠΊΡΠΎΠ²ΠΎΠΎΠ±ΡΠ°ΡΠ΅Π½ΠΈΡ Π² Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠΌ ΠΌΠΎΠ·Π³Π΅. Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ β ΠΎΡΠ΅Π½ΠΈΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Ρ
Π»ΠΎΡΠΈΠ΄Π° Π»ΠΈΡΠΈΡ Π΄Π»Ρ ΠΏΡΠ΅Π΄ΠΎΡΠ²ΡΠ°ΡΠ΅Π½ΠΈΡ Π³ΠΈΠ±Π΅Π»ΠΈ Π²ΡΡΠΎΠΊΠΎΡΡΠ²ΡΡΠ²ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΠΊ Π³ΠΈΠΏΠΎΠΊΡΠΈΠΈ Π½Π΅ΠΉΡΠΎΠ½ΠΎΠ² Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ° Π² ΠΏΠΎΡΡΡΠ΅Π°Π½ΠΈΠΌΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΌ ΠΏΠ΅ΡΠΈΠΎΠ΄Π΅ ΠΏΠΎΡΠ»Π΅ Π²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠΉ ΠΎΡΡΠ°Π½ΠΎΠ²ΠΊΠΈ ΡΠ΅ΡΠ΄ΡΠ°.Β ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΡΡΠ°Π½ΠΎΠ²ΠΊΡ ΡΠ΅ΡΠ΄ΡΠ° Ρ Π²Π·ΡΠΎΡΠ»ΡΡ
ΠΊΡΡΡ-ΡΠ°ΠΌΡΠΎΠ² Π½Π° 10 ΠΌΠΈΠ½ΡΡ Π²ΡΠ·ΡΠ²Π°Π»ΠΈ ΠΏΡΡΠ΅ΠΌ Π²Π½ΡΡΡΠΈΡΠΎΡΠ°ΠΊΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΠ΅ΡΠ΅ΠΆΠ°ΡΠΈΡ ΡΠΎΡΡΠ΄ΠΈΡΡΠΎΠ³ΠΎ ΠΏΡΡΠΊΠ° ΡΠ΅ΡΠ΄ΡΠ° Ρ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠ΅ΠΉ ΡΠ΅Π°Π½ΠΈΠΌΠ°ΡΠΈΠ΅ΠΉ. 9-ΡΠΈ ΠΆΠΈΠ²ΠΎΡΠ½ΡΠΌ Π²Π²ΠΎΠ΄ΠΈΠ»ΠΈ ΡΠ°ΡΡΠ²ΠΎΡ 4,2% LiCl Π·Π° 1 ΡΠ°Ρ Π΄ΠΎ ΠΎΡΡΠ°Π½ΠΎΠ²ΠΊΠΈ ΡΠ΅ΡΠ΄ΡΠ° (40 ΠΌΠ³/ΠΊΠ³ Π²/Π±), Π½Π° 1-Π΅ ΠΈ Π½Π° 2-Π΅ ΡΡΡΠΊΠΈ ΠΏΠΎΡΠ»Π΅ ΡΠ΅Π°Π½ΠΈΠΌΠ°ΡΠΈΠΈ (20 ΠΌΠ³/ΠΊΠ³ Π²/Π±, ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ). 9 Π½Π΅Π»Π΅ΡΠ΅Π½ΡΡ
ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
Π² ΡΠ΅ ΠΆΠ΅ ΡΡΠΎΠΊΠΈ ΠΏΠΎΠ»ΡΡΠ°Π»ΠΈ ΡΠΊΠ²ΠΈΠ²Π°Π»Π΅Π½ΡΠ½ΡΠ΅ Π΄ΠΎΠ·Ρ ΡΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ°ΡΡΠ²ΠΎΡΠ° Ρ
Π»ΠΎΡΠΈΠ΄Π° Π½Π°ΡΡΠΈΡ. ΠΠΎΠ½ΡΡΠΎΠ»Π΅ΠΌ ΡΠ»ΡΠΆΠΈΠ»ΠΈ Π»ΠΎΠΆΠ½ΠΎΠΏΠ΅ΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΊΡΡΡΡ (n=10). Π§Π΅ΡΠ΅Π· 7 Π΄Π½Π΅ΠΉ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΌΠΎΡΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΠΎΡΠ΅Π½ΠΈΠ»ΠΈ ΡΠΈΡΠ»ΠΎ ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ±Π½ΡΡ
Π½Π΅ΠΉΡΠΎΠ½ΠΎΠ² Π² ΠΏΠΎΠ»ΡΡ
Π‘Π1 ΠΈ Π‘Π3/Π‘Π4 Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ° Π½Π° ΡΡΠ΅Π·Π°Ρ
, ΠΎΠΊΡΠ°ΡΠ΅Π½Π½ΡΡ
ΠΊΡΠ΅Π·ΠΈΠ»ΠΎΠ²ΡΠΌ ΡΠΈΠΎΠ»Π΅ΡΠΎΠ²ΡΠΌ ΠΏΠΎ ΠΠΈΡΡΠ»Ρ. Π ΠΎΡΠ΄Π΅Π»ΡΠ½ΠΎΠΉ ΡΠ΅ΡΠΈΠΈ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠΎΠ² Ρ ΠΏΠΎΠΌΠΎΡΡΡ Western-Blot Π°Π½Π°Π»ΠΈΠ·Π° Π² ΡΡΠΈ ΠΆΠ΅ ΡΡΠΎΠΊΠΈ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π»ΠΈ Π²Π»ΠΈΡΠ½ΠΈΠ΅ Ρ
Π»ΠΎΡΠΈΠ΄Π° Π»ΠΈΡΠΈΡ Π½Π° ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ Π±Π΅Π»ΠΊΠ° GSK3Ξ² (ΠΊΠΈΠ½Π°Π·Π° Π³Π»ΠΈΠΊΠΎΠ³Π΅Π½ΡΠΈΠ½ΡΠ°Π·Ρ ΠΊΠΈΠ½Π°Π·Ρ-3) Π² ΡΠΊΠ°Π½ΠΈ ΠΌΠΎΠ·Π³Π°. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΡΠΈ Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΡΡΡΠ°Π½ΠΎΠ²ΠΈΠ»ΠΈ, ΡΡΠΎ 10-ΠΌΠΈΠ½ΡΡΠ½Π°Ρ ΠΎΡΡΠ°Π½ΠΎΠ²ΠΊΠ° ΡΠ΅ΡΠ΄ΡΠ° ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΡ ΡΠΈΡΠ»Π° ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ±Π½ΡΡ
Π½Π΅ΠΉΡΠΎΠ½ΠΎΠ² Π² ΠΏΠΎΠ»Π΅ Π‘Π1 Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ° β Π½Π° 37,5% (p0,001), Π² ΠΏΠΎΠ»Π΅ Π‘Π3/Π‘Π4 β Π½Π° 12,9% (p0,05). ΠΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ LiCl ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΠ»ΠΎ ΠΊ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΡ ΡΠΈΡΠ»Π° ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ±Π½ΡΡ
Π½Π΅ΠΉΡΠΎΠ½ΠΎΠ² Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ° Ρ ΡΠ΅Π°Π½ΠΈΠΌΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΊΡΡΡ Π² ΠΏΠΎΠ»Π΅ Π‘Π1 Π½Π° 37% (p0,01), Π² ΠΏΠΎΠ»Π΅ Π‘Π3/Π‘Π4 β Π½Π° 11,5% (p0,1) ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ Π½Π΅Π»Π΅ΡΠ΅Π½ΡΠΌΠΈ ΠΆΠΈΠ²ΠΎΡΠ½ΡΠΌΠΈ.Β ΠΡΠΈ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ Π±Π΅Π»ΠΊΠ° GSK3Ξ² ΡΡΡΠ°Π½ΠΎΠ²ΠΈΠ»ΠΈ, ΡΡΠΎ Ρ ΡΠ΅Π°Π½ΠΈΠΌΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
, ΠΏΠΎΠ»ΡΡΠ°Π²ΡΠΈΡ
Ρ
Π»ΠΎΡΠΈΠ΄ Π»ΠΈΡΠΈΡ, ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ Π΅Π³ΠΎ ΡΠΎΡΡΠΎΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΡΠΎΡΠΌΡ Π² ΡΠΊΠ°Π½ΠΈ ΠΌΠΎΠ·Π³Π° Π±ΡΠ»ΠΎ Π²ΡΡΠ΅ Π½Π° 180% ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΠΊΠΎΠ½ΡΡΠΎΠ»Π΅ΠΌ (Ρ0,05), ΠΈ Π½Π° 150% Π²ΡΡΠ΅, ΡΠ΅ΠΌ Ρ Π½Π΅Π»Π΅ΡΠ΅Π½Π½ΡΡ
ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
(Ρ0,05).Β ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠ²Π΅Π΄Π΅Π½ΠΈΠ΅ Ρ
Π»ΠΎΡΠΈΠ΄Π° Π»ΠΈΡΠΈΡ Π² ΠΏΠΎΡΡΡΠ΅Π°Π½ΠΈΠΌΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΌ ΠΏΠ΅ΡΠΈΠΎΠ΄Π΅ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΠ»ΠΎ ΠΊ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΠΉ Π½Π΅ΠΉΡΠΎΠΏΡΠΎΡΠ΅ΠΊΡΠΈΠΈ Π² Π½Π΅ΠΉΡΠΎΠ½Π°Π»ΡΠ½ΡΡ
ΠΏΠΎΠΏΡΠ»ΡΡΠΈΡΡ
Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ°. ΠΡΠΎΡ ΡΡΡΠ΅ΠΊΡ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ΠΌ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΡΠΎΡΡΠΎΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΡΠΎΡΠΌΡ Π±Π΅Π»ΠΊΠ° GSK3Ξ². ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎ Π²ΡΡΠΎΠΊΠΎΠΌ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»Π΅ Π»ΠΈΡΠΈΡ Π΄Π»Ρ ΠΏΡΠΎΡΠΈΠ»Π°ΠΊΡΠΈΠΊΠΈ ΠΈ Π»Π΅ΡΠ΅Π½ΠΈΡ Π½Π΅ΠΉΡΠΎΠ΄Π΅Π³Π΅Π½Π΅ΡΠ°ΡΠΈΠ²Π½ΡΡ
Π½Π°ΡΡΡΠ΅Π½ΠΈΠΉ, Π²ΡΠ·Π²Π°Π½Π½ΡΡ
Π²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠΉ ΠΎΡΡΠ°Π½ΠΎΠ²ΠΊΠΎΠΉ ΠΊΡΠΎΠ²ΠΎΠΎΠ±ΡΠ°ΡΠ΅Π½ΠΈΡ.
Brain Morphological Changes in COVID-19
The aim of the study was to identify the pathomorphology of brain damage in patients who died of COVID-19.Material and methods. Autopsy reports and autopsy brain material of 17 deceased patients with premortem conο¬rmed COVID-19 infection were analyzed. Fatal cases in which COVID-19 was the major cause of death were included in the study. Five people were diagnosed with cerebral infarction. Organ samples were taken for histological examination during autopsy. Sections were stained with hematoxylin and eosin and by Nissl to assess brain histopathology. To study the vascular basal membranes the PAS reaction was used, to detect ο¬brin in vessels phosphotungstic acid-hematoxylin (PTAH) staining was used, to determine DNA in nuclei sections were stained according to Feulgen, to detect RNA in neuronal nuclei and cytoplasm sections were stained with methyl green-pyronin. Immunohistochemical study of a neuronal marker, nuclear protein NeuN, was performed to assess neuronal damage.Results. The signs of neuronal damage found in patients who died of COVID-19 included nonspeciο¬c changes of nerve cells (acute swelling, retrograde degeneration, karyolysis and cytolysis, βghost' cells, neuronophagia and satellitosis) and signs of circulatory disorders (perivascular and pericellular edema, diapedesis, congested and engorged microvasculature).Conclusion. Brain histopathological data indicate damage to the central nervous system in COVID-19 patients. Ischemic stroke in patients with COVID-19 is mostly caused by a combination of hypoxia resulting from respiratory failure and individual risk factors, including cerebrovascular atherosclerosis and hypertension
ΠΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π° ΠΏΡΠΈ COVID-19
The aim of the study was to identify the pathomorphology of brain damage in patients who died of COVID-19.Material and methods. Autopsy reports and autopsy brain material of 17 deceased patients with premortem conο¬rmed COVID-19 infection were analyzed. Fatal cases in which COVID-19 was the major cause of death were included in the study. Five people were diagnosed with cerebral infarction. Organ samples were taken for histological examination during autopsy. Sections were stained with hematoxylin and eosin and by Nissl to assess brain histopathology. To study the vascular basal membranes the PAS reaction was used, to detect ο¬brin in vessels phosphotungstic acid-hematoxylin (PTAH) staining was used, to determine DNA in nuclei sections were stained according to Feulgen, to detect RNA in neuronal nuclei and cytoplasm sections were stained with methyl green-pyronin. Immunohistochemical study of a neuronal marker, nuclear protein NeuN, was performed to assess neuronal damage.Results. The signs of neuronal damage found in patients who died of COVID-19 included nonspeciο¬c changes of nerve cells (acute swelling, retrograde degeneration, karyolysis and cytolysis, βghost' cells, neuronophagia and satellitosis) and signs of circulatory disorders (perivascular and pericellular edema, diapedesis, congested and engorged microvasculature).Conclusion. Brain histopathological data indicate damage to the central nervous system in COVID-19 patients. Ischemic stroke in patients with COVID-19 is mostly caused by a combination of hypoxia resulting from respiratory failure and individual risk factors, including cerebrovascular atherosclerosis and hypertension.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ β Π²ΡΡΠ²ΠΈΡΡ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΈ ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½ΠΈΡ Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π° ΡΠΌΠ΅ΡΡΠΈΡ
ΠΎΡ COVID-19.ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΡΠΎΠ²Π΅Π»ΠΈ Π°Π½Π°Π»ΠΈΠ· ΠΏΡΠΎΡΠΎΠΊΠΎΠ»ΠΎΠ² Π²ΡΠΊΡΡΡΠΈΠΉ ΠΈ Π°ΡΡΠΎΠΏΡΠΈΠΉΠ½ΠΎΠ³ΠΎ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π° Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π° 17 ΡΠΌΠ΅ΡΡΠΈΡ
ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ ΠΏΡΠΈΠΆΠΈΠ·Π½Π΅Π½Π½ΠΎ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½Π½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠ΅ΠΉ Π‘OVID-19. Π ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π²ΠΊΠ»ΡΡΠΈΠ»ΠΈ ΡΠ»ΡΡΠ°ΠΈ Π»Π΅ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΈΡΡ
ΠΎΠ΄Π°, Π² ΠΊΠΎΡΠΎΡΡΡ
COVID-19 ΡΠ²ΠΈΠ»Π°ΡΡ ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠΉ ΠΏΡΠΈΡΠΈΠ½ΠΎΠΉ ΡΠΌΠ΅ΡΡΠΈ. Π£ 5 ΡΠ΅Π»ΠΎΠ²Π΅ΠΊ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΡΠΎΠ²Π°Π»ΠΈ ΠΈΠ½ΡΠ°ΡΠΊΡ Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π°. ΠΡΡΠΎΡΠΊΠΈ ΠΎΡΠ³Π°Π½ΠΎΠ² Π΄Π»Ρ Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΈΠ·ΡΠΌΠ°Π»ΠΈ Π² Ρ
ΠΎΠ΄Π΅ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΎΠ°Π½Π°ΡΠΎΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ. ΠΠ»Ρ Π²ΡΡΠ²Π»Π΅Π½ΠΈΡ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π° ΡΡΠ΅Π·Ρ ΠΎΠΊΡΠ°ΡΠΈΠ²Π°Π»ΠΈ Π³Π΅ΠΌΠ°ΡΠΎΠΊΡΠΈΠ»ΠΈΠ½ΠΎΠΌ ΠΈ ΡΠΎΠ·ΠΈΠ½ΠΎΠΌ ΠΈ ΠΏΠΎ ΠΌΠ΅ΡΠΎΠ΄Ρ ΠΠΈΡΡΠ»Ρ. ΠΠ»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠΎΡΡΠΎΡΠ½ΠΈΡ Π±Π°Π·Π°Π»ΡΠ½ΡΡ
ΠΌΠ΅ΠΌΠ±ΡΠ°Π½ ΡΠΎΡΡΠ΄ΠΎΠ² ΠΏΡΠΈΠΌΠ΅Π½ΡΠ»ΠΈ Π¨ΠΠ-ΡΠ΅Π°ΠΊΡΠΈΡ, Π΄Π»Ρ Π²ΡΡΠ²Π»Π΅Π½ΠΈΡ ΡΠΈΠ±ΡΠΈΠ½Π° Π² ΡΠΎΡΡΠ΄Π°Ρ
ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ ΠΎΠΊΡΠ°ΡΠΊΡ Π³Π΅ΠΌΠ°ΡΠΎΠΊΡΠΈΠ»ΠΈΠ½ΠΎΠΌ Π .Π’.Π.Π. ΡΠΎΡΡΠΎΠ²ΠΎΠ»ΡΡΡΠ°ΠΌΠΎΠ²ΡΠΌ ΠΊΠΈΡΠ»ΡΠΌ, Π΄Π»Ρ Π²ΡΡΠ²Π»Π΅Π½ΠΈΡ ΠΠΠ Π² ΡΠ΄ΡΠ°Ρ
ΡΡΠ΅Π·Ρ ΠΎΠΊΡΠ°ΡΠΈΠ²Π°Π»ΠΈ ΠΏΠΎ Π€Π΅Π»ΡΠ³Π΅Π½Ρ, Π΄Π»Ρ Π²ΡΡΠ²Π»Π΅Π½ΠΈΡ Π ΠΠ Π² ΡΠ΄ΡΠ°Ρ
ΠΈ ΡΠΈΡΠΎΠΏΠ»Π°Π·ΠΌΠ΅ Π½Π΅ΠΉΡΠΎΠ½ΠΎΠ² ΡΡΠ΅Π·Ρ ΠΊΡΠ°ΡΠΈΠ»ΠΈ ΠΌΠ΅ΡΠΈΠ»ΠΎΠ²ΡΠΌ Π·Π΅Π»Π΅Π½ΡΠΌ ΠΏΠΈΡΠΎΠ½ΠΈΠ½ΠΎΠΌ. ΠΠ»Ρ ΠΎΡΠ΅Π½ΠΊΠΈ ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½ΠΈΡ Π½Π΅ΠΉΡΠΎΠ½ΠΎΠ² ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ΅ (ΠΠΠ₯) ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π½Π΅ΠΉΡΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΌΠ°ΡΠΊΠ΅ΡΠ° β ΡΠ΄Π΅ΡΠ½ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠ° NeuN.Β Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΡΡΠ²ΠΈΠ»ΠΈ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΈ ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½ΠΈΡ Π½Π΅ΠΉΡΠΎΠ½ΠΎΠ² Ρ ΡΠΌΠ΅ΡΡΠΈΡ
ΠΎΡ COVID-19, Π·Π°ΠΊΠ»ΡΡΠ°ΡΡΠΈΠ΅ΡΡ Π² Π½Π΅ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡΡ
Π½Π΅ΡΠ²Π½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ (ΠΎΡΡΡΠΎΠ΅ Π½Π°Π±ΡΡ
Π°Π½ΠΈΠ΅, ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΠΎΠ΅ ΡΠ°Π·Π΄ΡΠ°ΠΆΠ΅Π½ΠΈΠ΅, ΠΊΠ°ΡΠΈΠΎΡΠΈΡΠΎΠ»ΠΈΠ·, ΠΊΠ»Π΅ΡΠΊΠΈ-ΡΠ΅Π½ΠΈ, Π½Π΅ΠΉΡΠΎΠ½ΠΎΡΠ°Π³ΠΈΡ ΠΈ ΡΠ°ΡΡΠ΅Π»ΠΈΡΠΎΠ·) ΠΈ ΠΏΡΠΈΠ·Π½Π°ΠΊΠ°Ρ
Π½Π°ΡΡΡΠ΅Π½ΠΈΡ ΠΊΡΠΎΠ²ΠΎΠΎΠ±ΡΠ°ΡΠ΅Π½ΠΈΡ (ΠΏΠ΅ΡΠΈΠ²Π°ΡΠΊΡΠ»ΡΡΠ½ΡΠΉ ΠΈ ΠΏΠ΅ΡΠΈΡΠ΅Π»Π»ΡΠ»ΡΡΠ½ΡΠΉ ΠΎΡΠ΅ΠΊ, Π΄ΠΈΠ°ΠΏΠ΅Π΄Π΅Π·Π½ΡΠ΅ ΠΊΡΠΎΠ²ΠΎΠΈΠ·Π»ΠΈΡΠ½ΠΈΡ, ΡΡΠ°Π·Ρ, ΠΏΠΎΠ»Π½ΠΎΠΊΡΠΎΠ²ΠΈΠ΅ ΡΠΎΡΡΠ΄ΠΎΠ² ΠΌΠΈΠΊΡΠΎΡΠΈΡΠΊΡΠ»ΡΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΡΡΠ»Π°).ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π° ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎ ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½ΠΈΠΈ ΡΠ΅Π½ΡΡΠ°Π»ΡΠ½ΠΎΠΉ Π½Π΅ΡΠ²Π½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ Ρ Π±ΠΎΠ»ΡΠ½ΡΡ
COVID-19. ΠΡΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΈΠ½ΡΡΠ»ΡΡ Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ COVID-19 Π²ΠΎ ΠΌΠ½ΠΎΠ³ΠΎΠΌ ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½ ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΠ΅ΠΌ Π³ΠΈΠΏΠΎΠΊΡΠΈΠΈ, ΡΠ°Π·Π²ΠΈΠ²Π°ΡΡΠ΅ΠΉΡΡ Π²ΡΠ»Π΅Π΄ΡΡΠ²ΠΈΠ΅ Π΄ΡΡ
Π°ΡΠ΅Π»ΡΠ½ΠΎΠΉ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎΡΡΠΈ, ΠΈ ΡΡΡΠ΅ΡΡΠ²ΡΡΡΠΈΡ
Ρ Π±ΠΎΠ»ΡΠ½ΠΎΠ³ΠΎ ΡΠ°ΠΊΡΠΎΡΠΎΠ² ΡΠΈΡΠΊΠ° β Π°ΡΠ΅ΡΠΎΡΠΊΠ»Π΅ΡΠΎΠ·Π° Π°ΡΡΠ΅ΡΠΈΠΉ ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΡ Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π° ΠΈ Π³ΠΈΠΏΠ΅ΡΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π±ΠΎΠ»Π΅Π·Π½ΠΈ
Sepsis-Associated Encephalopathy (Review)
International studies demonstrate an annual increase in the frequency and impact of sepsis in intensive care units (ICU). Cerebral dysfunction, or so-called sepsis-associated encephalopathy (SAE), is one of the earliest signs of sepsis, as well as its common complication. Up to 70% of patients with sepsis have symptoms of encephalopathy [1, 2]. A direct link between the SAE and an increased mortality rate is a major concern; more than a half of sepsis survivors experience continuous memory and concentration impairment [3β5]. Diagnosis of the cerebral dysfunction in sepsis is often difficult because of lack of specific biomarkers and frequent prescription of sedatives to critically ill patients. Clinical manifestations of SAE are diverse, may vary from simple malaise and lack of concentration to deep coma. The literature discusses probable mechanisms of formation and development of septic encephalopathy, such as oxidative stress, inflammation, mitochondrial and endothelial dysfunction, increased permeability of the blood-brain barrier, impairment of macro- and microcirculation, changes in neirotransmission, activation of microglia. The lack of clear data and consensus about the etiology and mechanisms of SAE at present does not permit predicting its development and prescribing a specific therapy. The review discusses current understanding of the causes of septic encephalopathy, methods of diagnosis, the main clinical manifestations, key mediators, and pathophysiological mechanisms. A hypothesis is proposed that poses a contribution of aromatic microbial metabolites (AMM) of phenol and indole nature (products of bacterial biodegradation of tyrosine and tryptophan) to the development of brain dysfunction. The fields of exploratory studies, which might open perspectives in the diagnosis, as well as new approaches in the treatment of SAE are outlined
Neuroprotective Eο¬ect of Lithium Chloride in Rat Model of Cardiac Arrest
Lithium chloride, which is used for the treatment of bipolar disorders, has a neuroprotective eο¬ect in conditions associated with acute and chronic circulatory disorders.The purpose of the study: to investigate the eο¬cacy of lithium chloride for the prevention of post-resuscitation death of hippocampal neurons during the post-resuscitation period.Material and methods. Cardiac arrest for 10 minutes was evoked in mature male rats by intrathoracic clumping of the vascular bundle of the heart, followed by resuscitation. 40 mg/kg or 20 mg/kg of 4,2% lithium chloride (LiCl) was injected intraperitoneally 1 hour before cardiac arrest, on the 1st and 2nd day after resuscitation (n=9). Untreated animals received equivalent doses of saline (n=9). Rats after a sham surgery served as a reference group (n=10). The number of viable neurons in the CA1 and CA3/CA4 ο¬elds of the hippocampus was estimated in slides stained with cresyl violet by day 6 or 7 postresuscitation. In a separate series of experiments, at the same terms, we studied the eο¬ect of lithium chloride on the protein content of GSK3Ξ² (glycogen synthase kinase) in brain tissue using Western-Blot analysis.Results.Β Histological assay showed that a 10-minute cardiac arrest resulted in a decrease in the number of viable neurons in the hippocampal CA1 ο¬eld β by 37.5% (P0.001), in the CA3/CA4 ο¬eld β by 12.9% (P0.05) vs. the reference group. Lithium treatment increased the number of viable neurons in resuscitated rats β in the CA1 ο¬eld by 37% (P<0.01), in the CA3/CA4 ο¬eld β by 11.5% (P0.1) vs. the untreated animals. It was found that lithium caused an increase in phosphorylated form of GSK3Ξ²: by 180% vs. the reference group (P[1]0.05), and by 150% vs. the untreated animals (P0.05).Conclusion. Lithium treatment leads to a pronounced neuroprotection in the neuronal populations of the hippocampus post-resuscitation. This eο¬ect may be due to an increase in the content of the phosphorylated form of GSK3Ξ² protein. The results indicate a high potential of lithium for the prevention and treatment of neurodegenerative disorders caused by a temporary arrest of blood circulation
Prognostic Value and Therapeutic Potential of Brain-Derived Neurotrophic Factor (BDNF) in Brain Injuries (Review)
Neurotrophins are proteins that play an important role in the nervous system functioning by regulating cell proliferation, differentiation, processes of neuronal survival and death, and by participating in the mechanisms of neuronal plasticity. The brain-derived neurotrophic factor (BDNF) is one of the most well-described representatives of the neurotrophin family, which has received close attention over recent years. It is considered one of the key mediators of neuronal survival and recovery, and a drop of the BDNF level is considered a common mechanism underlying the development of various neurodegenerative diseases. The review discusses changes in BDNF levels in ischemic and traumatic brain damage, the prospects of its use in the clinical practice as a marker of brain dysfunction, as well as the possibility of its use for the treatment of post-ischemic encephalopathies