Criteria for bioreactor comparison and operation standardisation during process development for mammalian cell culture

Platas Barradas O, Jandt U, Da Minh Phan L, et al. Criteria for bioreactor comparison and operation standardisation during process development for mammalian cell culture. BMC Proceedings. 2011;5(8)

Characterisation of cultivation of the human cell line AGE1.HN.AAT

Skerhutt EM, Scholz S, Niklas J, et al. Characterisation of cultivation of the human cell line AGE1.HN.AAT. BMC Proceedings. 2011;5(8)

Prozessführungs- und Kultivierungsstrategien für die humane Produktionszelllinie AGE1.HN

Diese Arbeit basiert auf der Entwicklung von Prozessführungsstrategien für die systematische Charakterisierung und Optimierung von Säugetierzellkulturen, insbesondere für die Produktionszelllinie AGE1.HN. Diese Zelllinie wurde innerhalb des Verbundprojektes SysLogics -Systems Biology of Cell Culture for Biologics- mit dem Ziel untersucht, quantitative und systematische Daten über den Zellmetabolismus zu generieren. Die in dieser Arbeit entwickelten Strategien gelten als Grundlage für systematische Studien mit Säugetierzellen, deren Charakterisierung aus der Sicht der Ingenieurswissenschaften und Implementierung für die Systembiologieforschung.This work focuses on the development of process and cultivation strategies for a systematic characterization and optimization of mammalian cell cultures, especially for the novel human industrial cell line AGE1.HN. This cell line was studied within the collaborative project SysLogics -Systems Biology of Cell Culture for Biologics- with the final goal of generating quantitative data on mammalian cell metabolism. The strategies developed in this work establish a solid basis for systematic studies with mammalian production cell lines, by allowing for their characterization from an engineering point of view, and the implementation of results in systems biology research

Model-based strategy for cell culture seed train layout verified at lab scale

Cell culture seed trains—the generation of a sufficient viable cell number for the inoculation of the production scale bioreactor, starting from incubator scale—are time- and cost-intensive. Accordingly, a seed train offers potential for optimization regarding its layout and the corresponding proceedings. A tool has been developed to determine the optimal points in time for cell passaging from one scale into the next and it has been applied to two different cell lines at lab scale, AGE1.HN(AAT) and CHO-K1. For evaluation, experimental seed train realization has been evaluated in comparison to its layout. In case of the AGE1.HN(AAT) cell line, the results have also been compared to the formerly manually designed seed train. The tool provides the same seed train layout based on the data of only two batches