Spectral unmixing of multiply stained fluorescence samples T

The widespread use of fluorescence microscopy along with the vast library of available fluorescent stains and staining methods has been extremely beneficial to researchers in many fields, ranging from material sciences to plant biology. In clinical diagnostics, the ability to combine different markers in a given sample allows the simultaneous detection of the expression of several different molecules, which in turn provides a powerful diagnostic tool for pathologists, allowing a better classification of the sample at hand. The correct detection and separation of multiple stains in a sample is achieved not only by the biochemical and optical properties of the markers, but also by the use of appropriate hardware and software tools. In this chapter, we will review and compare these tools along with their advantages and limitations

Continuous synthesis of drug-loaded nanoparticles using microchannel emulsification and numerical modeling: Effect of passive mixing

By using interdigital microfluidic reactors, monodisperse poly(d, l lactic-co-glycolic acid) nanoparticles (NPs) can be produced in a continuous manner and at a large scale (~10 g/h). An optimized synthesis protocol was obtained by selecting the appropriated passive mixer and fluid flow conditions to produce monodisperse NPs. A reduced NP polydispersity was obtained when using the microfluidic platform compared with the one obtained with NPs produced in a conventional discontinuous batch reactor. Cyclosporin, an immunosuppressant drug, was used as a model to validate the efficiency of the microfluidic platform to produce drug-loaded monodisperse poly(d, l lactic-co-glycolic acid) NPs. The influence of the mixer geometries and temperatures were analyzed, and the experimental results were corroborated by using computational fluid dynamic three-dimensional simulations. Flow patterns, mixing times, and mixing efficiencies were calculated, and the model supported with experimental results. The progress of mixing in the interdigital mixer was quantified by using the volume fractions of the organic and aqueous phases used during the emulsification–evaporation process. The developed model and methods were applied to determine the required time for achieving a complete mixing in each microreactor at different fluid flow conditions, temperatures, and mixing rates

Using Wavelets to reject background in Dark Matter experiments

A method based on wavelet techniques has been developed and applied to background rejection in the data of the IGEX dark matter experiment. The method is presented and described in some detail to show how it efficiently rejects events coming from noise and microphonism through a mathematical inspection of their recorded pulse shape. The result of the application of the method to the last data of IGEX is presented.Comment: 14 pages, 8 figures. Submitted to Astrop. Phy

Neutron background at the Canfranc Underground Laboratory and its contribution to the IGEX-DM dark matter experiment

A quantitative study of the neutron environment in the Canfranc Underground Laboratory has been performed. The analysis is based on a complete set of simulations and, particularly, it is focused on the IGEX-DM dark matter experiment. The simulations are compared to the IGEX-DM low energy data obtained with different shielding conditions. The results of the study allow us to conclude, with respect to the IGEX-DM background, that the main neutron population, coming from radioactivity from the surrounding rock, is practically eliminated after the implementation of a suitable neutron shielding. The remaining neutron background (muon-induced neutrons in the shielding and in the rock) is substantially below the present background level thanks to the muon veto system. In addition, the present analysis gives us a further insight on the effect of neutrons in other current and future experiments at the Canfranc Underground Laboratory. The comparison of simulations with the body of data available has allowed to set the flux of neutrons from radioactivity of the Canfranc rock, (3.82 +- 0.44) x 10^{-6} cm^{-2} s^{-1}, as well as the flux of muon-induced neutrons in the rock, (1.73 +- 0.22(stat) \+- 0.69(syst)) x 10^{-9} cm^{-2} s^{-1}, or the rate of neutron production by muons in the lead shielding, (4.8 +- 0.6 (stat) +- 1.9 (syst)) x 10^{-9} cm^{-3} s^{-1}.Comment: 17 pages, 8 figures, elsart document class; final version to appear in Astroparticle Physic

Status of the ANAIS Dark Matter Project at the Canfranc Underground Laboratory

The ANAIS experiment aims at the confirmation of the DAMA/LIBRA signal. A detailed analysis of two NaI(Tl) crystals of 12.5 kg each grown by Alpha Spectra will be shown: effective threshold at 1 keVee is at reach thanks to outstanding light collection and robust PMT noise filtering protocols and the measured background is well understood down to 3 keVee, having quantified K, U and Th content and cosmogenic activation in the crystals. A new detector was installed in Canfranc in March 2015 together with the two previous modules and preliminary characterization results will be presented. Finally, the status and expected sensitivity of the full experiment with 112 kg will be reviewed.Comment: Contributed to the 11th Patras Workshop on Axions, WIMPs and WISPs, Zaragoza, June 22 to 26, 201

Prevalencia de hipertensión arterial en la población española a partir de la encuesta europea de salud

La hipertensión arterial (HTA) constituye un problema de salud pública en todos los países desarrollados, lo cual es debido por una parte a su elevada frecuencia y por otra a la gravedad que pueden tener sus posibles complicaciones. Constituye uno de los motivos de consulta más frecuente en Atención Primaria (AP); y es en este punto donde se realizan su diagnóstico, intervención terapéutica y seguimiento. El objetivo de nuestro estudio ha sido conocer la prevalencia de HTA en la población española y conocer los factores de riesgo sociodemográficos que se relacionan con la misma. Para ello, se ha llevado a cabo un estudio descriptivo y transversal a partir de la base de datos recogidos en la Encuesta Europea de Salud de 2014. El análisis de los resultados pone de relieve que una cuarta parte de la población española es hipertensa y prueba la relación de causalidad entre la HTA y los factores de riesgo edad, baja actividad física y la condición de sobrepeso/obesidad. Estos dos últimos, factores de riesgo modificables y por tanto objetivos de necesaria intervención para conseguir una disminución de la prevalencia de hipertensión y una mayor calidad y esperanza de vida en nuestra población de estudio