Seminar 2: Biochemistry based techniques

Este documento contiene un pdf con las diapositivas utilizadas para el seminario 2, dentro de la asignatura de Bioquímica y Biología Molecular, para los alumnos de 1 curso del grado de Medicina. El seminario es una introducción a las técnicas de laboratorio que se utilizan en un laboratorio de bioquímica y biología molecular, incluyendo técnicas como la cromatografía, la electroforesis de DNA o de proteínas.This document contains a pdf with the slides used for seminar 2, within the subject of Biochemistry and Molecular Biology, for students of 1 year of the Medicine degree. The seminar is an introduction to laboratory techniques used in a biochemistry and molecular biology laboratory, including techniques such as chromatography, DNA or protein electrophoresis

Seminar 2_Clinical Enzimology

Este documento ha sido revisado por el Servicio de política Linguística. Su labor ha sido muy importante para la correción del documento.Este documento contiene un pdf con las diapositivas utilizadas para el seminario 2, dentro de la asignatura de Integración de Bioquímica y Biología Molecular, para los alumnos de 2º curso del grado de Medicina. El seminario habla de los ensayos clínicos realizados en sangre, del valor diagnóstico de determinadas activades enzimáticas, y como realizar correctamente un análisis de sangre. Una segunda parte de este seminario trata sobre las isoenzimas.This document contains a pdf with the slides used for the seminar 2, within the subject of Integration of Biochemistry and Molecular Biology, for students in the 2nd year of the Medicine degree. The seminar talks about clinical test carried out on blood, the diagnostic value of certain enzymatic activities, and how to correctly perform a blood test. The second part of this seminar deals with isoenzymes

Calpains, the proteases of two faces controlling the epithelial homeostasis in mammary gland

Calpain-1 and calpain-2 are calcium-dependent Cys-proteases ubiquitously expressed in mammalian tissues with a processive, rather than degradative activity. They are crucial for physiological mammary gland homeostasis as well as for breast cancer progression. A growing number of evidences indicate that their pleiotropic functions depend on the cell type, tissue and biological context where they are expressed or dysregulated. This review considers these standpoints to cover the paradoxical role of calpain-1 and -2 in the mammary tissue either, under the physiological conditions of the postlactational mammary gland regression or the pathological context of breast cancer. The role of both calpains will be examined and discussed in both conditions, followed by a brief snapshot on the present and future challenges for calpains, the two-gateway proteases towards tissue homeostasis or tumor development

MET-EGFR dimerization in lung adenocarcinoma is dependent on EGFR mtations and altered by MET kinase inhibition.

Advanced lung cancer has poor survival with few therapies. EGFR tyrosine kinase inhibitors (TKIs) have high response rates in patients with activating EGFR mutations, but acquired resistance is inevitable. Acquisition of the EGFR T790M mutation causes over 50% of resistance; MET amplification is also common. Preclinical data suggest synergy between MET and EGFR inhibitors. We hypothesized that EGFR-MET dimerization determines response to MET inhibition, depending on EGFR mutation status, independently of MET copy number. We tested this hypothesis by generating isogenic cell lines from NCI-H1975 cells, which co-express L858R and T790M EGFR mutations, namely H1975L858R/T790M (EGFR TKI resistant); H1975L858R (sensitized) and H1975WT (wild-type). We assessed cell proliferation in vitro and tumor growth/stroma formation in derived xenograft models in response to a MET TKI (SGX523) and correlated with EGFR-MET dimerization assessed by Förster Resonance Energy Transfer (FRET). SGX523 significantly reduced H1975L858R/T790M cell proliferation, xenograft tumor growth and decreased ERK phosphorylation. The same was not seen in H1975L858R or H1975WT cells. SGX523 only reduced stroma formation in H1975L858R. SGX523 reduced EGFR-MET dimerization in H1975L858R/T790M but induced dimer formation in H1975L858R with no effect in H1975WT. Our data suggests that MET inhibition by SGX523 and EGFR-MET heterodimerisation are determined by EGFR genotype. As tumor behaviour is modulated by this interaction, this could determine treatment efficacy

Lab book_BBM_22_23

Este documento ha sido revisado por el Servicio de política Linguística. Su labor ha sido muy importante para la correción del documento.Este documento contiene el cuadernillo de prácticas que utilizan los alumnos de Bioquímica y Biología Molecular, asignatura de primer año del grado de Medicina. El documento describe las diferentes sesiones de prácticas que se realizan en el laboratorio. Para cada práctica, se incluye una pequeña base teórica, así como el protocolo de cada sesión de laboratorio. Además, en algunos casos se incluyen preguntas relacionadas con las prácticas, para ser resueltas por el alumno.This document contains the lab book used by Biochemistry and Molecular Biology students, a first-year subject of the Medicine degree. The document describes the different practical sessions that are carried out in the laboratory. For each practical session, a small theoretical content is included, as well as the protocol of each laboratory session. In addition, in some cases, questions related to the sessions are included, to be resolved by the student

Lesson 6: Enzymatic reactions

Este documento ha sido revisado por el Servicio de política Linguística. Su labor ha sido muy importante para la correción del documento.Este documento contiene un pdf con las diapositivas utilizadas en el tema 6 de los alumnos de primero del Grado de Medicina, dentro de la asignatura Bioquímica y Biología Molecular. Se describen los conceptos y funciones de las enzimas, las propiedades de las mismas, así como la nomenclatura y clasificación, mecanismos de la acción enzimática y el concepto de cofactor enzimático.This document contains a pdf doc with the slides used in lesson 6 of the first-year students of the Medicine Degree, within the Biochemistry and Molecular Biology subject. The lesson is about enzymes and comprises the concept and functions of enzymes, their properties, the nomenclature, classification, and mechanisms of enzymatic action. Finally, the concept of enyzme cofactors is described

LAb book IBBC_22_23

Este documento ha sido revisado por el Servicio de política Linguística. Su labor ha sido muy importante para la correción del documento.Este documento contiene el cuadernillo de prácticas que utilizan los alumnos de Integración Bioquímica y Biología Molecular, asignatura de segundo año del grado de Medicina. El documento describe las diferentes sesiones de prácticas que se realizan en el laboratorio. Para cada práctica, se incluye una pequeña base teórica, así como el protocolo de cada sesión de laboratorio. Además, en algunos casos se incluyen preguntas relacionadas con las prácticas, para ser resueltas por el alumno.This document contains the lab book used by the Integration of Biochemistry and Clinical Biochemistry students, a second-year subject of the Medicine degree. The document describes the different practical sessions that are carried out in the laboratory. For each practical session, a small theoretical content is included, as well as the protocol of each laboratory session. In addition, in some cases, questions related to the sessions are included, to be solved by the student

Lesson 7: Enzymatic kinetics

Este documento ha sido revisado por el Servicio de política Linguística. Su labor ha sido muy importante para la correción del documento.Este documento contiene un pdf con las diapositivas utilizadas en el tema 7 de los alumnos de primero del Grado de Medicina, dentro de la asignatura Bioquímica y Biología Molecular. Se describen los conceptos de cinética enzimática y la ecuación de Michaelis Menten. También se describe el fenómeno de cooperatividad y el alosterismo enzimático.This document contains a pdf doc with the slides used in lesson 7 for first-year students of the Medicine Degree, within the Biochemistry and Molecular Biology subject. This lesson describes the concepts of enzyme kinetics and the Michaelis Menten equation. The phenomenon of cooperativity and enzymatic allosterism are also described

KIF22 coordinates CAR and EGFR dynamics to promote cancer cell proliferation

The cell junction–associated receptor CAR coordinates the dynamics between EGFR signaling and the cytoskeleton during cell proliferation.</jats:p