Oxidative Stress: Mechanistic Insights into Inherited Mitochondrial Disorders and Parkinson's Disease

Oxidative stress arises when cellular antioxidant defences become overwhelmed by a surplus generation of reactive oxygen species (ROS). Once this occurs, many cellular biomolecules such as DNA, lipids, and proteins become susceptible to free radical-induced oxidative damage, and this may consequently lead to cellular and ultimately tissue and organ dysfunction. Mitochondria, as well as being a source of ROS, are vulnerable to oxidative stress-induced damage with a number of key biomolecules being the target of oxidative damage by free radicals, including membrane phospholipids, respiratory chain complexes, proteins, and mitochondrial DNA (mt DNA). As a result, a deficit in cellular energy status may occur along with increased electron leakage and partial reduction of oxygen. This in turn may lead to a further increase in ROS production. Oxidative damage to certain mitochondrial biomolecules has been associated with, and implicated in the pathophysiology of a number of diseases. It is the purpose of this review to discuss the impact of such oxidative stress and subsequent damage by reviewing our current knowledge of the pathophysiology of several inherited mitochondrial disorders together with our understanding of perturbations observed in the more commonly acquired neurodegenerative disorders such as Parkinson’s disease (PD). Furthermore, the potential use and feasibility of antioxidant therapies as an adjunct to lower the accumulation of damaging oxidative species and hence slow disease progression will also be discussed

Developmental responses of bread wheat to changes in ambient temperature following deletion of a locus that includes FLOWERING LOCUS T1

FLOWERING LOCUS T (FT) is a central integrator of environmental signals that regulates the timing of vegetative to reproductive transition in flowering plants. In model plants, these environmental signals have been shown to include photoperiod, vernalization, and ambient temperature pathways, and in crop species, the integration of the ambient temperature pathway remains less well understood. In hexaploid wheat, at least 5 FT‐like genes have been identified, each with a copy on the A, B, and D genomes. Here, we report the characterization of FT‐B1 through analysis of FT‐B1 null and overexpression genotypes under different ambient temperature conditions. This analysis has identified that the FT‐B1 alleles perform differently under diverse environmental conditions; most notably, the FT‐B1 null produces an increase in spikelet and tiller number when grown at lower temperature conditions. Additionally, absence of FT‐B1 facilitates more rapid germination under both light and dark conditions. These results provide an opportunity to understand the FT‐dependent pathways that underpin key responses of wheat development to changes in ambient temperature. This is particularly important for wheat, for which development and grain productivity are sensitive to changes in temperature

Understanding Town Centre Performance in Wales: Using GIS to Develop a Tool for Benchmarking

Welsh Government policy establishes town centres as central places of community activity and local prosperity, recognising the positive impact towns have on the local economy and the well-being and cohesion felt amongst local communities. In light of this, recent declines in the usage of town centres are a major cause for concern. These have not been experienced uniformly across all towns, with some towns out-performing others. This paper applies principles outlined in Welsh Government’s Planning Policy Wales to develop a tool which classifies a sample of 71 towns and cities in Wales based on their centre and catchment characteristics. Catchment areas have been delineated using a Spatial Interaction Model to account for complex consumer behaviours and competition between centres. The tool identifies six distinct types of towns alongside key socio-economic catchment area characteristics. Once developed, we demonstrate our tool’s application by exploring variations in town centre performance between and within each town type. Case study examples exemplify how policymakers may use this tool to benchmark between towns, evaluating the suitability of a town’s retail offering based on its performance relative to the benchmark, guiding decisions relating to the types of businesses and uses a town should pursue to improve its appeal to its catchment community. In conclusion, several recommendations to policymakers are suggested