TOTAL POLYPHENOLS, FLAVONOIDS CONTENTS AND ANTIOXIDANT ACTIVITY OF ROSA SP. GENOTIPES FROM DIFFERENT ALTITUDE OF ROMANIAN REGIONS

Eight wild rose hip genotypes from different altitudes varying from 3m to 902m were analyzed in order to evaluate the total polyphenols, flavonoids content and the antioxidant activity. The Rosa spp.rosehips collected from the Northeastern and the Southeasternof Romanianwere, as follows: Rosa canina, R. caesia, R. corymbifera, R. micrantha, R. nitidula, R. rubiginosa, R. subcanina, and R. vosagiaca. In some genotypes, the level of flavonoids and polyphenol content increased with the increasing altitude while in other it was observed a decrease. Polyphenol content reached a maximum of 144.36 mg GAE/g DW in R. rubiginosa whereas the lowest content of 61.72 mg GAE/g DW was recorded in R. caesia. The highest polyphenol content was reached at altitude of 860 m. The amount of flavonoids content ranged between 7.32 mg CE/g DW and 19.45 CE/g DW in R. caesia and in R. nitidula, respectively. The radical scavenging capacities of Rosa genotypes extracts were not positively correlated with altitude, except the R. corymbifera extractswhere the antioxidant activity increased with the increase of altitude

ENZYMATIC ACTIVITY AND SOLUBLE PROTEIN CONTENT IN SEEDLINGS OF CALENDULA OFFICINALIS L. UNDER SALT STRESS

[EN] Enzymatic activity and soluble protein content in relation to salt stress tolerance were investigated in Calendula officinalis seedlings after 24 days of treatment with different salt treatments, including NaCl, CaCl2, MgCl2 and mixtures of them. The marigold seedlings were used in order to investigate the possible salt-inducible responses and the possible alleviative role of calcium and magnesium salts in respect with adverse salinity conditions. Activity of superoxide dismutase (SOD; EC 1.15.1.1) and peroxidase (POD; EC 1.11.1.7) generally slightly decreased under salt treatments, with minor variations from the value recorded for control series and within applied treatments. Catalase (CAT; EC 1.11.1.6) activity was stimulated by NaCl salinity and MgCl2 addition; its activity was found to be lowest under calcium and magnesium chloride treatments solelyOprica, L.; Vicente Meana, Ó.; Boscaiu Neagu, MT.; Grigore, MN. (2016). ENZYMATIC ACTIVITY AND SOLUBLE PROTEIN CONTENT IN SEEDLINGS OF CALENDULA OFFICINALIS L. UNDER SALT STRESS. Journal of Plant Development (Online). 23:71-79. http://hdl.handle.net/10251/91887S71792

Characterizing the effects of salt stress in Calendula officinalis L

[EN] In this study the effects of salt stress on growth and several stress markers were investigated in the ornamental and medicinal plant Calendula officinalis. One-month-old plants were submitted to increasing salt concentrations, up to 150 mM NaCl, for a period of 30 days. Salinity affected growth in terms of relative reduction of stem length and fresh weight of the plants, but water content remained unchanged indicating a certain tolerance to low and mild NaCl concentrations. Although Na+ and Cl- increased in parallel to increasing salinity, the levels of K+ and Ca2+ showed no significant change, while Mg2+ levels recorded a twofold increase upon the ap- plication of the highest salt concentration. Other measured para- meters showed a more significant change, notably proline levels, which registered a nine-fold increase in the presence of 150 mM NaCl. In conclusion, although plants suffered from salt stress, as shown by the degradation of photosynthetic pigments and induction of oxidative stress (increased MDA levels), they continued their ve- getative growth under low concentrations of salt. The main mechanisms of response to salt stress in this species appear to be based on the maintenance of K+ and Ca2+ homeostasis and the accumulation of proline as a functional osmolyte.Kozminska, A.; Al Hassan, M.; Kumar, D.; Oprica, L.; Martinelli, F.; Grigore, MN.; Vicente, O.... (2017). Characterizing the effects of salt stress in Calendula officinalis L. Journal of Applied Botany and Food Quality. 90:323-329. doi:10.5073/JABFQ.2017.090.040S3233299

THE IMPACT OF CoFeO 4 NANOPARTICLES ON SOLUBLE PROTEIN CONTENT AT WHITE ROT FUNGUS Phanerochaete chrysosporium

Experimental investigation focused on influence of different CoFeO4 nanoparticles concentrations on soluble protein and electrophoretic pattern of Phanerochaete chrysosporium fungus. Number of electrophoretic fraction is higher in older mycelium P. chrysosporium 14-day-old compared to that 7-day-old. Moreover, there is an increase in staining intensity of polypeptides of mycelium 14-day-old fact confirmed also by the higher amount of soluble protein. On the other hand, there are no visible differences as regards the different concentrations of nanoparticles effect on the polypeptides pattern

IMPACT OF INORGANIC SALT SOLUTIONS ON ANTIOXIDATIVE ENZYMES ACTIVITY AND PHOTOSYNTHETIC PIGMENTS CONTENT IN TRIGONELLA FOENUM-GRAECUM SEEDLINGS

Fenugreek (Trigonella foenum-graecum) is known to have several pharmacological effects such as hypoglycemia, hypocholesterolemia, gastroprotective, chemopreventive, antioxidant, antiinflammatory, antipyretic and appetite stimulation attributes. This plant is known to contain many secondary metabolites like alkaloids, flavonoids, salicylate, and nicotinic acid. Present investigation was undertaken to study the effect of salinity on fenugreek antioxidative defense mechanism. The response of the superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities as well as the pigment content in fenugreek seedlings was investigated after 24 days of treatment with inorganic salt solutions. It was used for treatment different concentration of NaCl alone and in combination with CaCl 2 as well as MgCl 2 . The fenugreek treatment was effected to elucidate the adverse effect of NaCl salinity and its possible amelioration by CaCl 2 or MgCl 2 . Generally, all treatments diminished the activity of antioxidant enzymes take in the study with one exception (150mM NaCl+10mM CaCl 2 in case of SOD). Only a few concentrations (50mM NaCl, 100mM NaCl, 50mM NaCl+10mM CaCl 2 , 150mM NaCl+10mM CaCl 2 , 100mM NaCl+20 MgCl 2 ) increased the fenugreek soluble protein content. Both CaCl 2 and MgCl 2 treatments applied alone and in combination determined an increase of photosynthetic pigments (Chl a, Chl b and carotenoids) contents compared with contro

Influence of the attack of the fungus Polystigma rubrum (pers.) dc (red leaf spot) on nutritional value of fruits in different plum cultivars

This paper carried out of the influence of Polystigma rubrum attack on the soluble and insoluble glucides content in the plum cultivars, with different degrees of resistance to fungus attack: Poroabe and Vinete româneşti (sensitive cultivars), Renclod violet and Grase româneşti (middle-resistant cultivars) and Renclod verde (resistant cultivar), during the year 2008. Analyzing the results we observed that soluble glucides and insoluble glucides content with one exception was higher in healthy fruits comparatively with the attacked fruit. In generally, glucides was low in attacked fruit, but cannot establish a correlation between glucides content and the degree of resistance of the plum cultivars to attack of Polystigma rubru

INFLUENCE OF Polystigma rubrum (Pers.) D.C ATTACK ON SOME BIOCHEMICAL PARAMETERS IN DIFFERENT PLUM CULTIVARS

The aim of this study is to evaluate the effects of the influence of Polystigma rubrum attack on some enzymes (catalase, peroxidase and polyphenol oxydase) activity from healthy and attacked leaves of the following plum cultivars with different degrees of resistance to diseases attack: Poroabe and Vinete româneúti (sensitive cultivars), Renclod violet and Grase româneúti (middle-resistant cultivars) and Renclod verde (resistant cultivar), during the year 2008. The pathogen Polystigma rubrum determined a peroxidase activity variation in all plum cultivars according to these phenophases registering a growth of its in the initial attack phase and a decrease in the intermediate and final phases, in the attcked leveas. The peroxidase activity was higher in healthy leaves comparatively with the attacked leaves. The evolution of catalase activity depending on the attack evolution but can established modifications from one cultivars to another. Analyzing the dynamic of polyphenol oxydase activity we observed that Polystigma rubrum produced some variations of this enzyme activity in all plum cultivars and recorded significant increases in the attacked leaves in the initial phase and terminal phase of attack of this fungus. In generally, it can not established a correlation between oxidoreductases activity and the degree of resistance of the plum cultivars to attack of Polystigma rubrum

EVALUATION OF SOME PHYTOCHEMICAL CONSTITUENTS AND THE ANTIOXIDANT ACTIVITY IN SIX ROSE HIPS SPECIES COLLECTED FROM DIFFERENT ALTITUDE OF SUCEAVA DISTRICT

In this study, species of rose hips fruits were assayed for the composition of polyphenol, flavonoids, anthocyanins contents as well as the antioxidant activity. Samples of rose hips representing six species (Rosa pendulina, R. tomentosa, R. canina, R. rubiginosa, R. corymbifera and R. nitidula) were collected from spontaneous flora of Suceava district. The smallest content of total polyphenols was recorded in R. nitidula, both in seeds (3.11mg GAE/g DW) and pulp (23.99 mg GAE/g DW). The maximum concentration of total polyphenols was found in R. pendulina, both in seeds (17.68 mg GAE/g DW) and pulp (71.48 mg GAE/g DW). The most abundant anthocyanin content in pulp of all specie studied was in R. canina (16.60 mg%). The relevant differences between pulp and seed regarding flavonoid content, about 7-fold were reached at R. tomentosa collected from Vatra Dornei. The results revealed that antioxidant activity of pulp extract from R. pendulina showed the largest scavenging activity while the lowest scavenging capacity was recorded for R. nitidula

THE EFFECT OF SOME RHIZOBACTERIAN STRAINS ON SOLUBLE PROTEINS CONTENT IN SOYBEANS (GLYCINE MAX L. MERR.)

Now it is an accepted fact that plant growth-promoting rhizobacteria (PGPR) can increase the productivity of several crops. The main objective of the present study was to find if there are any differences in protein content in the seeds of soybean (Glycine max L. MERR.). Using spectrophotometric methods for analyzing the protein contents and electrophoretic methods for qualitative analysis it was observed that no major modifications occur in protein spectrum. Looking at the quantitative side there was a small improvement in protein quantity

INFLUENCE DU CHAMP ÉLECTROMAGNÉTIQUE SUR L’ACTIVITÉ DE LA CATALASE ET DE LA PEROXYDASE DANS DES CULTURES MIXTES DE CHAETOMIUM GLOBOSUM ET TRICHODERMA VIRIDE

Les auteurs de cette étude présentent les résultats de leurs recherches sur l’influence du champ électromagnétique (uniforme et pulsatoire) sur l’activité de la catalase et de la peroxydase en cultures mixtes de Chaetomium globosum et Trichoderma viride, à 7 et 14 jours d’ensemencement. Il a été constaté que l’évolution de ces enzymes dépend du type de champ électromagnétique et de l’âge de la culture