Optimalisation of species identification of common carp (Cyprinus carpio) using SYBR® green I real-time PCRmethod

European Union Member States, together with a number of countries around the world, places great emphasis on ensuring the protection of consumers as a potential food allergic from food allergies. to inform consumers that their product may contain any of the risk of allergenic ingredients. For species identification of fish and fish products as a potential food allergens are used by many analytical methods as well as their authentication. We are in our work applied the method of SYBR® Green I. Real-Time PCR. We focused on pre-designed molecular - genetic marker of common carp (Cyprinus carpio), which comes from the mtDNA control D - loop area. We analyzed its presence in DNA isolates from the 5 kinds of freshwater fish, diluted to 10 % concentration. Results of using the optimized SYBR ® Green I Real-Time PCR method for species identification common carp (Cyprinus carpio) indicate to its suitability

Aligned nanofibres made of poly(3-hydroxybutyrate) grafted to hyaluronan for potential healthcare applications

In this work, a hybrid copolymer consisting of poly(3-hydroxybutyrate) grafted to hyaluronic acid (HA) was synthesised and characterised. Once formed, the P(3HB)-g-HA copolymer was soluble in water allowing a green electrospinning process. The diameters of nanofibres can be tailored by simply varying the Mw of polymer. The optimization of the process allowed to produce fibres of average diameter in the range of 100-150 nm and low polydispersity. The hydrophobic modification has not only increased the fibre diameter, but also the obtained layers were homogenous. At the nanoscale, the hybrid copolymer exhibited an unusual hairy topography. Moreover, the hardness and tensile properties of the hybrid were found to be superior compared to fibres made of unmodified HA. Particularly, this reinforcement was achieved at the longitudinal direction. Additionally, this work reports the use in the composition of a water-soluble copolymer containing photo cross-linkable moieties to produce insoluble materials post-electrospinning. The derivatives as well as their nanofibrous mats retain the biocompatibility of the natural polymers used for the fabrication

Odhad polohy mobilných zariadení

This contribution describes mathematical model (kinematics) for Mobile Robot carriage. The mathematical model is fully parametric. Model is designed universally for any measures three or four wheeled carriage. The next conditions are: back wheels are driving-wheel, front wheels change angle of Robot turning. Position of the front wheel gives the actual position of the robot. Position of the robot is described by coordinates x, y and by angle of the front wheel α in reference position. Main reason for model implementation is indoor navigation. We need some estimation of robot position especially after turning of the Robot. Next use is for outdoor navigation especially for precising GPS information.Príspevok popisuje matematický model (kinematiku) podvozku mobilného robota. Matematický model je plne parametrický a je navrhnutý univerzálne pre ľubovoľné rozmery troj- alebo štvor-kolesového podvozku s prihliadnutím na tieto podmienky: zadné kolesá sú hnacie, predné kolesá menia smer pohybu. Využitie pre navigáciu vo vnútorných priestoroch. Matematický odhad je potrebný hlavne pri otáčaní robota. Ďalšie využitie môže byť aj pri navigácii vo vonkajších priestoroch na spresnenie GPS informácie. Pozícia predných kolies dáva aktuálnu pozíciu robota. Poloha robota je popísaná súradnicami x, y a uhlom predných kolies α

Location Estimation of Mobile Devices

This contribution describes mathematical model (kinematics) for Mobile Robot carriage. The mathematical model is fully parametric. Model is designed universally for any measures three or four wheeled carriage. The next conditions are: back wheels are driving-wheel, front wheels change angle of Robot turning. Position of the front wheel gives the actual position of the robot. Position of the robot is described by coordinates x, y and by angle of the front wheel α in reference position. Main reason for model implementation is indoor navigation. We need some estimation of robot position especially after turning of the Robot. Next use is for outdoor navigation especially for precising GPS information

Detection of Lupine (Lupinus spp. L.) as a food allergen using three methods: end-point PCR, Real-Time PCR and Elisa

<span style="font-size:10.0pt;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;; mso-fareast-font-family:&quot;Times New Roman&quot;;mso-ansi-language:SK;mso-fareast-language: SK;mso-bidi-language:AR-SA">The aim of this work was to compare three methods for the detection and quantification of lupine as an allergen in food. The methods that were used in this work were the direct method: ELISA and the indirect methods: end-point PCR and real-time PCR. We examined the detection limit (the sensitivity with which we can detect the presence of the allergen in a&nbsp;sample) and the reliability for performing an analysis. We used 17 samples of plant species from a processing plant for dehydrated soups production and lupine samples from lupine processing companies.</span><span style="font-size:12.0pt;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;; mso-fareast-font-family:&quot;Times New Roman&quot;;mso-ansi-language:EN-GB;mso-fareast-language: SK;mso-bidi-language:AR-SA"> </span><span style="font-size:10.0pt; font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;; mso-ansi-language:EN-GB;mso-fareast-language:SK;mso-bidi-language:AR-SA" lang="EN-GB">Its practical use is wide and it is used mainly in the bakery industry, in the manufacture of confectionery, pasta, sauces, as a substitute for soy and also in the production of gluten-free food, because it does not contain gluten.</span><span style="font-size:12.0pt;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;; mso-fareast-font-family:&quot;Times New Roman&quot;;mso-ansi-language:EN-GB;mso-fareast-language: SK;mso-bidi-language:AR-SA" lang="EN-GB"> </span><span style="font-size:10.0pt; font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;; mso-ansi-language:EN-GB;mso-fareast-language:SK;mso-bidi-language:AR-SA" lang="EN-GB">Lupine, however, is also included in the list of 14&nbsp;allergenic substances, which in accordance with the EU legislation must be listed on food labels. The high risk group, which suffers from primary sensitization or cross-reaction with peanuts, are allergic patients. In the EU, people who are allergic to peanuts range from 0.7 to 1.5%. In experiment 1, we detected the presence of lupine using primers for the detection of &alpha;- and &delta;-conglutine in the samples, using the end-point PCR method and the detection limit of this reaction was at the level of 100 ppm. For the vizualization of the DNA fragments, we used a&nbsp;2% agarose gel and UV visualizer.</span><span style="font-size:12.0pt; font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;; mso-ansi-language:EN-GB;mso-fareast-language:SK;mso-bidi-language:AR-SA" lang="EN-GB"> </span><span style="font-size:10.0pt;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;; mso-fareast-font-family:&quot;Times New Roman&quot;;mso-ansi-language:EN-GB;mso-fareast-language: SK;mso-bidi-language:AR-SA" lang="EN-GB">In experiment 2 we detected lupine using the TaqMan real-time PCR reaction and primers for the detection of &alpha; and &delta;-conglutine at the level of 10 ppm of lupine in sample. The CP values of lupine using primers for the detection of &alpha;-conglutine was 24.85 &plusmn; 0.12 and the reliability equation was R² = 0.9767. The CP lupine values using primers for the detection of &delta;-conglutine was 22.52 &plusmn; 0.17 and the reliability equation was R² = 0.9925. In experiment 3, we used a&nbsp;sandwich ELISA method for the detection of lupine and the detection limit was within the range of 2-30 ppm and the reliability of the method according to the reliability equation was R² = 0.9975. The high sensitivity and equation of reliability justify the use of these methods for the detection and quantification of lupine in practice. The&nbsp;most sensitive indirect method for the detection of lupine in our study was the method of real-time TaqMan PCR with a detection limit&nbsp; 10&nbsp;000-10 ppm of lupine. The most sensitive direct methot was ELISA with detection limit 2-30 ppm of lupine.</span

Synthesis and Physicochemical Characterization of Undecylenic Acid Grafted to Hyaluronan for Encapsulation of Antioxidants and Chemical Crosslinking

In this work, a new amphiphilic derivative made of 10-undecylenic acid grafted to hyaluronan was prepared by mixed anhydrides. The reaction conditions were optimized, and the effect of the molecular weight (Mw), reaction time, and the molar ratio of reagents was explored. Using this methodology, a degree of substitution up to 50% can be obtained. The viscosity of the conjugate can be controlled by varying the substitution degree. The physicochemical characterization of the modified hyaluronan was performed by infrared spectroscopy, Nuclear Magnetic Resonance, Size-Exclusion Chromatography combined with Multiangle Laser Light Scattering (SEC-MALLS), and rheology. The low proton motility and self-aggregation of the amphiphilic conjugate produced overestimation of the degree of substitution. Thus, a novel method using proton NMR was developed. Encapsulation of model hydrophobic guest molecules, coenzyme Q10, curcumin, and &alpha;-tocopherol into the micellar core was also investigated by solvent evaporation. HA-UDA amphiphiles were also shown to self-assemble into spherical nanostructures (about 300 nm) in water as established by dynamic light scattering. Furthermore, HA-UDA was crosslinked via radical polymerization mediated by ammonium persulphate (APS/TEMED). The cross-linking was also tested by photo-polymerization catalyzed by Irgacure 2959. The presence of the hydrophobic moiety decreases the swelling degree of the prepared hydrogels compared to methacrylated-HA. Here, we report a novel hybrid hyaluronan (HA) hydrogel system of physically encapsulated active compounds and chemical crosslinking for potential applications in drug delivery