5 research outputs found
SDS-Page characterization of some elite cowpea (Vigna unguiculata L. Walp) varieties
The shortcomings of genotype x environment interaction necessitated the use of molecular methods in characterizing many plant species and in determining their phylogenetic relationships. In this study, some selected cowpea lines (27 varieties) from Obafemi Awolowo University, Ile – Ife, the Institute of Agricultural Research (IAR), Samaru, Kaduna and Genetic Resource Centre, IITA, Ibadan were characterized using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) profiling. The protein banding profiles of the 27 cowpea varieties were scored and subjected to cluster analysis using Ward's minimum-variance method (WMVM) for dendrogram grouping. The dendrogram generated from the SDS-PAGE profiles grouped the varieties into seven clusters at 52% similarity coefficient. Hence, the biochemical characterization revealed more precise discrimination among the 27 cowpea varieties studied.
Keywords: Cowpea, electrophoretic banding profiles, dendrogram grouping, total protein
Capacity of Nigerian national institutions to conduct biotechnology research to improve livestock productivity
Biotechnology development has been the subject of interest in Nigeria over the past decade. As
part of the study by the International Livestock Research Institute (ILRI) sponsored by the US
Agency for International Development (USAID) to investigate the development and/or prospects
of biotechnology tools as applied to animal science research in Nigeria, a survey aimed at assessing biotechnology capacity of Nigeria for animal research was conducted between June and July
2004. The survey used a structured questionnaire, interviews and personal visits to some
universities, polytechnics and national research institutions situated in strategic locations in
Nigeria. Though a general deterioration of facilities in most universities was noted, there are
quite a number of institutions identified to be capable of applying some biotechnology tools to
improve animal production in Nigeria. There are well-trained Nigerian researchers in the country
who when supplied with tools in biotechnology and financial support to carry out well-focused
or coordinated research can help propel the country towards self-sufficiency in animal production.
The role of ILRI and development investors like USAID in making significant intervention in
the attainment of this goal is highlighted
African suid genomes provide insights into the local adaptation to diverse African environments
African wild suids consist of several endemic species that represent ancient members of the family Suidae and have colonized diverse habitats on the African continent. However, limited genomic resources for African wild suids hinder our understanding of their evolution and genetic diversity. In this study, we assembled high-quality genomes of a common warthog (Phacochoerus africanus), a red river hog (Potamochoerus porcus), as well as an East Asian Diannan small-ear pig (Sus scrofa). Phylogenetic analysis showed that common warthog and red river hog diverged from their common ancestor around the Miocene/Pliocene boundary, putatively predating their entry into Africa. We detected species-specific selective signals associated with sensory perception and interferon signaling pathways in common warthog and red river hog, respectively, which contributed to their local adaptation to savannah and tropical rainforest environments, respectively. The structural variation and evolving signals in genes involved in T-cell immunity, viral infection, and lymphoid development were identified in their ancestral lineage. Our results provide new insights into the evolutionary histories and divergent genetic adaptations of African suids