18 research outputs found

    Comparative assessment of SSAP, AFLP and SSR markers for evaluation of genetic diversity of durum wheat (triticum turgidum l. var. durum)

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    Comparative assessment of genetic diversity of 122 durum wheat genotypes (Triticum turgidum L. var. durum) was performed using 73 SSAP polymorphic fragments, 123 AFLP polymorphic loci and 104 SSR alleles. SSAP and AFLP data showed a clear demarcation between the cultivars and landraces and SSR data classified cultivars and landraces according to their origin. Furthermore, the estimated genetic diversity of Iranian landraces was higher compared to the foreign entries and a loss of genetic diversity was observed from landraces to cultivars. This study determined that differences in genetic relationships revealed by SSAP, AFLP and SSR distances could not be attributed solely to differences in the level of polymorphism detected by each marker system. The molecular evidence of genetic diversity decrease of the durum wheat gene pool further strengthens the strategic relevance of undertaking appropriate genetic conservation measures for food security

    Ultrarapid Measurement of Diagnostic Antibodies by Magnetic Capture of Immune Complexes

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    Rapid point-of-care, antibody-based testing is not currently available for the diagnosis of most autoimmune and infectious diseases. Here we report a simple, robust and ultrafast fluid-phase immunocapture method for clinical measurements of antibody levels. This method employs neodymium magnetic sticks that capture protein A/G-coated paramagnetic beads bound to antibody-luciferase-labeled antigen complexes. We demonstrate the ability to effectively measure specific antibody levels in serum samples from patients with varied infectious or autoimmune disorders, and in the case of Sjögren's syndrome directly in saliva, requiring about a minute per assay. We also show the feasibility of coupling this method with a hand-held luminometer for portable testing. Our method offers the potential to quickly diagnose a multitude of autoimmune and infectious diseases in point-of-care settings
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