11 research outputs found
Cyclical Changes in Prolactin Levels among Infertile Women Attending University of Port Harcourt Teaching Hospital
Study was carried out to determine the effect of cyclical changes of prolactin concentrations on infertility in 3 groups of infertile subjects attending university of Port Harcourt teaching hospital for infertility treatments. Prolactin samples of the subjects were determined at follicular (day3), Midcycle (day14) and luteal phase (day21) using enzyme immune assay method (EIA). Prolactin was significantly increased in infertile subjects compared with the controls (Apparently fertile) suggesting an increase in prolactin concentration in infertile subjects. There was also cyclical changes observed in prolactin secretion with midcycle (day14) being the peak. Cyclical changes were also observed in prolactin concentration in both fertile and infertile subjects which peaked at midcycle (day14) suggesting that cycle of subjects should be taken into consideration in estimation, interpretation and investigation of infertility while the elevation of prolactin in the 3 study groups might be responsible for the infertility observed. @JASEMKeyword: Prolactin, immuno assay, follicular phase, midcycle, luteal phasesJ. Appl. Sci. Environ. Manage. Sept, 2011, Vol. 15 (3) 425 - 43
Intestinal parasites and haemoglobin concentrations in pregnant women attending Braithwaite Memorial Specialist Hospital, Port Harcourt
This study was carried out between January to June, 2014 at Braithwaite Memorial Specialist Hospital, Port Harcourt to determine the haemoglobin level and intestinal parasites in pregnant women attending antenatal clinic of the hospital. In total, 200 stool and blood samples were collected from the women. The stool samples were examined using direct wet preparation and formol ether concentration technique while the haemoglobin levels of the women were estimated using cyanmethaeoglobin technique. The overall prevalence of E. histolytical, A. Lumbricoides, Hookworm Spp, and T. trichuria was 8(4.00), 58(29.00), 8(4.00) and 8(4.00) respectively. The proportion of pregnant women with helminithic infestation was 60 (30.0%) while their mean Heamoglobin concentration was 10.85+0.32g/dl while the pregnant women without helminthic infestation (140(70.0%) had Heamoglobin concentration of 11.03+0.16g/dl (P<0.05). The study has shown that pregnant women with intestinal parasites had low haemoglobin level. This may be attributed to poor socioeconomic status of the women coupled with poor environmental sanitation and lack of clean portable water supply may have contributed to the high prevalence
Effect of Cassava based diet on lipids concentration in albino rats fed with crude oil contaminated diet
The study was carried out to ascertain the effect of a cassava based
diet (gari) on lipid profile in albino rats fed crude oil contaminated
diets by feeding diet contaminated with various concentrations of crude
oil mixed with 20% gari to albino rats to determine the protective
effect of gari. The lipid profile (Cholesterol, triglycerides, HDL
cholesterol and LDL cholesterol) were monitored in the animals. Gari
feeding at 20% caused dose dependent reduction in Cholesterol and LDL
cholesterol with dose dependent increases in Triglycerides and HDL
cholesterol in gari fed albino rats compared with Petroleum fed albino
rats (P<0.05) suggesting that gari reversed the effect of crude oil
on changes in lipid profile. Dose dependent increase in cholesterol,
LDL cholesterol and dose dependent decrease triglycerides and HDL
cholesterol was observed in petroleum fed rats compared with their
controls (P<0.05). The study showed that ingestion of petroleum
contaminated diet caused increase cholesterol and LDL cholesterol and
decreased triglycerides and HDL cholesterol, but supplementation of the
diet with 20%Gari lowered the increased concentrations of cholesterol
and LDL cholesterol observed in the Petroleum contaminated diet while
increasing the triglycerides and HDL cholesterol concentrations. This
study showed that feeding on gari diet caused reversed to changes in
lipid concentration caused by crude petroleum. \ua9 JASE
Cyclical Changes in Prolactin Levels among Infertile Women Attending University of Port Harcourt Teaching Hospital
Study was carried out to determine the effect of cyclical changes of
prolactin concentrations on infertility in 3 groups of infertile
subjects attending university of Port Harcourt teaching hospital for
infertility treatments. Prolactin samples of the subjects were
determined at follicular (day3), Midcycle (day14) and luteal phase
(day21) using enzyme immune assay method (EIA). Prolactin was
significantly increased in infertile subjects compared with the
controls (Apparently fertile) suggesting an increase in prolactin
concentration in infertile subjects. There was also cyclical changes
observed in prolactin secretion with midcycle (day14) being the peak.
Cyclical changes were also observed in prolactin concentration in both
fertile and infertile subjects which peaked at midcycle (day14)
suggesting that cycle of subjects should be taken into consideration in
estimation, interpretation and investigation of infertility while the
elevation of prolactin in the 3 study groups might be responsible for
the infertility observed
Effect of Cassava based diet on hepatic proteins in albino rats fed with crude oil contaminated diet
The study was carried out to ascertain the glucose effect of a cassava
based diet (garri) on crude oil hepatotoxicity in albino rats by
feeding diet contaminated with various concentrations of crude oil
mixed with 20% gari to determine the protective effect of gari. The
hepatic enzymes aspartate amino transferase (AST), alanine amino
transferase (ALT), gamma glutamyl transpeptidase (GGT) and alkaline
phosphatase (ALK PHOS) activities with albumin, Total protein and liver
/body weight were monitored in the animals. Gari feeding at 20% caused
dose dependent reduction in enzymes activities (ALT, AST, GGT and
ALKPHOS) with dose dependent increases in albumin and Protein in gari
fed albino rats compared with Petroleum fed albino rats (P<0.05)
suggesting that gari reversed the hepatotoxic effect of crude oil. Dose
dependent increase in enzymes activities and dose dependent decrease in
proteins was observed in petroleum fed rats compared with their
controls (P<0.05). The study showed that ingestion of petroleum
contaminated diet caused increase activities of liver enzymes namely
ALKPHOS, AST, ALT and GGT and decreased Protein concentrations, an
indicator of possible liver damage but supplementation of the diet with
20%Gari lowered the increased activities of ALKPHOS, AST, ALT and GGT
observed in the Petroleum contaminated diet while increasing the
Protein concentration. This study showed that feeding on gari diet
reversed the liver damage caused by crude petroleum as evidenced by
reduced release of liver enzymes through glucose effect
Haemoglobin Level and Intestinal Parasites in Pregnant Women in Eziama, Imo State of Nigeria
This prospective study was carried out between January to June, 2008 at Eziama in Isiala Mbano Local Government Area of Imo State to determine the haemoglobin level and intestinal parasites in pregnant women attending antenatal clinic. In total, 281 stool and blood samples were collected from the women. The stool samples were examined using direct wet preparation and formol ether concentration technique while the haemoglobin levels of the women were estimated using cyanmethaeoglobin technique. The overall prevalence of E. histolytical, A. Lumbricoides, Hookworm Spp, T. trichuria and G. lamblia was 10(3.6), 31(11.0), 29(10.3), 10(3.6) and 3(1.1) respectively. The proportion of pregnant women with helminithic infestation was 83 (29.6%) while their mean Heamoglobin concentration was 9.7+1.8g/dl while the pregnant women without helminthic infestation had Heamoglobin concentration of 10.4 + 1.3g/dl. The poor socioeconomic status of the women coupled with poor environmental sanitation and lack of clean portable water supply may have contributed to the high prevalence
Effects of exercise on growth hormone concentrations of female students in Madonna University
Human growth hormone is secreted in a pulsatile fashion, generally following a circadian rhythm. The growth hormone concentrations of 25 female students of Madonna University, Elele within ages groups 19-27years were determined before (Pre) and after (Post) exercise. Growth hormone levels were assayed using the commercially available Human Growth Hormone quantitative test kit whose principle is based on a solid phase enzyme-linked immunosorbent assay (ELISA). The data generated was calculated statistically using statistical package for social sciences (SPSS) version 17. The result showed significant difference in 5.45 + 0.97 ng/ml in pre exercise and 13.89 + 1.77 ng/ml in post exercise (P<0.05). There was no significant increase in growth hormone pre and Post exercise at different age groups 19-21years (13.91± 9.51 ng/ml and 14.78 ± 3.09 ng/ml), 22-24 (6.73 ±1.73 ng/ml and 11.73 ± 1.97ng/ml) and 25-27(4.77 ± 2.10ng/ml and 19.61 ± 7.54ng/ml).The study has shown that exercise can affect concentration of growth hormone in females
Comparative Study of the Protective Effect of Granulated Sugar and Garri Meal on Petroleum-induced Changes of Lipid Profiles in Albino Rats (Rattusnorvegicus)
Crude oil contaminated diet has been reported to induced changes in lipid profile of rats. In this study, 60 albino rats in 6 groups were fed with crude oil contaminated sugar-garri diets at varied concentrations to monitor petroleum-induce lipid profile changes using standard methods. The lipid profiles estimated were total cholesterols, triglycerides, high density lipoprotein cholesterol and Low density lipoproteincholesterol.The data showed that, the levels of total cholesterol (Mmol/l) increased from 2.89+0.08 in sugar fed rats compared with 3.05+0.12 in Gari fed rats. The concentration of triglycerides(Mmol/l) reduced from 1.10+0.51 in sugar fed rats to 1.06 + 0.03 in Gari fed rats while the high density lipoprotein cholesterol(Mmol/l) concentration of 2. 01+0.08 was significantly higher than 1.89 + 0.03 in Gari fed rats (P<0.05).There was no significant difference in Low density lipoprotein cholesterol (Mmol/l) concentrations of 0.68+0.11 in sugar fed rats compared with 0.83 + 0.10 in Gari fed rats. The effect of pre-treatment of the diet with sugar reduced petroleum-induce changes in lipid profile than gari during the exposure of the albino rats to the petroleum diet suggesting that sugarcould possibly ameliorate petroleum-induce changes in lipid profile as shown by the significantlowering of the cholesterol and Low density lipoprotein cholesterol than gari in albino rats. Keywords: lipid, Petroleum, Sugar, Gari, Cassava, Change
Cyclical Changes in Prolactin Levels among Infertile Women Attending University of Port Harcourt Teaching Hospital
Study was carried out to determine the effect of cyclical changes of
prolactin concentrations on infertility in 3 groups of infertile
subjects attending university of Port Harcourt teaching hospital for
infertility treatments. Prolactin samples of the subjects were
determined at follicular (day3), Midcycle (day14) and luteal phase
(day21) using enzyme immune assay method (EIA). Prolactin was
significantly increased in infertile subjects compared with the
controls (Apparently fertile) suggesting an increase in prolactin
concentration in infertile subjects. There was also cyclical changes
observed in prolactin secretion with midcycle (day14) being the peak.
Cyclical changes were also observed in prolactin concentration in both
fertile and infertile subjects which peaked at midcycle (day14)
suggesting that cycle of subjects should be taken into consideration in
estimation, interpretation and investigation of infertility while the
elevation of prolactin in the 3 study groups might be responsible for
the infertility observed
Effect of Cassava based diet on hepatic proteins in albino rats fed with crude oil contaminated diet
The study was carried out to ascertain the glucose effect of a cassava
based diet (garri) on crude oil hepatotoxicity in albino rats by
feeding diet contaminated with various concentrations of crude oil
mixed with 20% gari to determine the protective effect of gari. The
hepatic enzymes aspartate amino transferase (AST), alanine amino
transferase (ALT), gamma glutamyl transpeptidase (GGT) and alkaline
phosphatase (ALK PHOS) activities with albumin, Total protein and liver
/body weight were monitored in the animals. Gari feeding at 20% caused
dose dependent reduction in enzymes activities (ALT, AST, GGT and
ALKPHOS) with dose dependent increases in albumin and Protein in gari
fed albino rats compared with Petroleum fed albino rats (P<0.05)
suggesting that gari reversed the hepatotoxic effect of crude oil. Dose
dependent increase in enzymes activities and dose dependent decrease in
proteins was observed in petroleum fed rats compared with their
controls (P<0.05). The study showed that ingestion of petroleum
contaminated diet caused increase activities of liver enzymes namely
ALKPHOS, AST, ALT and GGT and decreased Protein concentrations, an
indicator of possible liver damage but supplementation of the diet with
20%Gari lowered the increased activities of ALKPHOS, AST, ALT and GGT
observed in the Petroleum contaminated diet while increasing the
Protein concentration. This study showed that feeding on gari diet
reversed the liver damage caused by crude petroleum as evidenced by
reduced release of liver enzymes through glucose effect