8 research outputs found
The production of species-specific highly unsaturated fatty acyl-containing LCOs from Rhizobium leguminosarum bv. trifolii is stringently regulated by nodD and involves the nodRL genes
Microbial Biotechnolog
Novel branched Nod factor structure results from a-(1-3) fucosyl transferase activity: the major lipo-chitin oligosaccharides from Mesorhizobium loti strain NZP2213 bear an a-(1-3) fucosyl substituent on a nonterminal backbone residue
Microbial Biotechnolog
Native structural and functional proteoform characterization of the prolyl-alanyl-specific endoprotease EndoPro from Aspergillus niger
Proteomic
Effect of pH and soybean cultivars on the quantitative analyses of soybean rhizobia populations
Microbial Biotechnolog
Comparative Proteome Analysis of Saccharomyces cerevisiae grown in chemostat cultures limited for glucose or ethanol
Heterologous protein production in the yeast Kluyveromyces lactis
Kluyveromyces lactis is both scientifically and biotechnologically one of the most important non-Saccharomyces yeasts. Its biotechnological significance builds on its history of safe use in the food industry and its well-known ability to produce enzymes like lactase and bovine chymosin on an industrial scale. In this article, we review the various strains, genetic techniques and molecular tools currently available for the use of K. lactis as a host for protein expression. Additionally, we present data illustrating the recent use of proteomics studies to identify cellular bottlenecks that impede heterologous protein expression
Heterologous protein production in the yeast Kluyveromyces lactis
Kluyveromyces lactis is both scientifically and biotechnologically one of the most important non-Saccharomyces yeasts. Its biotechnological significance builds on its history of safe use in the food industry and its well-known ability to produce enzymes like lactase and bovine chymosin on an industrial scale. In this article, we review the various strains, genetic techniques and molecular tools currently available for the use of K. lactis as a host for protein expression. Additionally, we present data illustrating the recent use of proteomics studies to identify cellular bottlenecks that impede heterologous protein expression