The 2015-2016 El Niño event: expected impact on food security and main response scenarios in East and Southern Africa

El Niño is a periodic climate phenomenon defined by anomalously warm sea surface temperatures in the eastern and central tropical Pacific Ocean which affect local weather worldwide and generally leads to increased drought risk at global level. In 2015, since July a strong El Niño is being observed with increasing intensity in September and October. It is expected to last for the first 3 months of 2016 and could reach a very high level of intensity in this period. To date, it has already affected climate in many parts of Asia and in the Northern parts of East Africa, causing serious rainfall deficits. Although the impact on agriculture is not directly proportional to the intensity of the climatic anomalies, the event is expected to impact East and Southern Africa in different ways. In East Africa, for the bimodal areas, El Niño events in the second half of the year usually lead to wetter than average conditions and are generally beneficial for agriculture. In other areas with a long crop season in the second half of the year, such as parts of Ethiopia, Sudan and Eritrea, it can cause drier than average conditions followed by rainfall at harvesting time causing drying problems. For both bimodal and single season zones it can lead to flooding in riverine areas and increase the risk of livestock diseases. On the contrary, in Southern Africa, strong El Niño events frequently cause drought and reduce crop production and this effect could be particularly dangerous considering the low crop production of this region in the 2014-2015 season. These risks need to be taken into consideration for response planning in East and Southern Africa and this report lists some main recommeJRC.H.4-Monitoring Agricultural Resource

Auto-protective redox buffering systems in stimulated macrophages

BACKGROUND: Macrophages, upon encounter with micro-organisms or stimulated by cytokines, produce various effector molecules aimed at destroying the foreign agents and protecting the organism. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are front line molecules exerting strong cytotoxic activities against micro-organisms and many cells, including macrophages themselves. Using cells of the murine macrophage cell line (RAW 264.7) stimulated in vitro with lipopolysaccharide (LPS) and/or interferon (IFN-γ), which induce strong endogenous NO production, we examined by which mechanisms a fraction of activated macrophages protect themselves from nitrosative stress and manage to escape destruction? RESULTS: We observed that survivors (10–50% depending on the experiments) had acquired a resistant phenotype being capable to survive when further exposed in vitro to an apoptosis inducing dose of the NO donor compound DETA-NO. These cells expressed an increased steady-state levels of Mn SOD, CuZn SOD and catalase mRNA (130–200%), together with an increased activity of the corresponding enzymes. Intracellular concentration of glutathione was also increased (× 3.5 fold at 6 hours, still maintained × 5.2 fold at 48 hours). Neither mRNA for glutathione peroxydase, γ-glutamylcysteine synthase and glutathione reductase, nor thioredoxine and thioredoxine reductase, were significantly modified. Additional experiments in which RAW 264.7 cells were stimulated with LPS and/or IFN-γ in the presence of relatively specific inhibitors of both Mn and Cu/Zn SOD, aminotriazol (ATZ) catalase inhibitor and buthionine sulfoximine (BSO) glutathione inhibitor, showed that inhibiting LPS-induced up-regulation of intracellular redox buffering systems also prevented acquisition of the resistant phenotype. CONCLUSIONS: Our data suggest a direct causal relationship between survival of a fraction of macrophages and a up-regulation of key sets of auto-protective intracellular redox buffering systems, occurring simultaneously with modulation of expression of apoptotic molecules of the Bcl(2)-Bcl-(XL)/Bax-Bad family

Subjective sleep dysfunction and insomnia symptoms in Parkinson's disease: Insights from a cross-sectional evaluation of the French CoPark cohort

Introduction: Twenty-seven to 80% of patients with Parkinson's Disease (PD) complain of subjective sleep dysfunction and insomnia symptoms. Our aim is to describe the prevalence and features of subjective sleep dysfunction and insomnia symptoms in patients with PD compared to other patients. Methods: Cross-sectional analysis of 636 adult PD patients compared to 143 age and sex-matched non-PD control patients consulting their general practitioners. Insomnia symptoms and other sleep features were assessed by the Pittsburgh Sleep Quality Index (PSQI), a global score > 5 defining impaired sleep. The Chi-square test or the Student's t-test were used to assess the potential clinical and demographic differences between groups and between PD patients with vs. without sleep dysfunction. Logistic regression analysis was employed to test multivariate effects. Results: Sleep dysfunction and insomnia symptoms were more frequent in PD patients compared to control patients (63 vs. 45%, p = 0.001). Female gender, PD duration, presence of depression and anxiety were associated with the presence of insomnia in PD. Subjective sleep efficiency, habitual sleep quality, sleep disturbance and daytime dysfunction, but not sleep latency, were reduced in PD patients compared to controls. Conclusions: The prevalence of sleep dysfunction is higher in PD than in other general medical conditions. Insomnia in PD seems to affect sleep maintenance and consolidation, but not sleep onset.Fil: Ratti, Pietro Luca. Université Paul Sabatier; Francia. Inserm; Francia. Sleep and Epilepsy Center; Suiza. Toulouse University Hospital; FranciaFil: Negre Pages, Laurence. Toulouse University Hospital; Francia. Université Paul Sabatier; FranciaFil: Pérez Lloret, Santiago. Toulouse University Hospital; Francia. Université Paul Sabatier; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Manni, Raffaele. Istituto di Ricovero e Cura a Carattere Scientifico. Istituto Neurologico Nazionale a Carattere Scientifico; ItaliaFil: Damier, Philippe. Universite de Nantes; FranciaFil: Tison, Francois. Universite de Bordeaux; FranciaFil: Destée, Alain. Centre Hospitalier Universitaire de Lille. Pôle de Neurologie. Service de Neurologie et pathologie du mouvement; FranciaFil: Rascol, Olivier. Toulouse University Hospital; Francia. Inserm; Francia. Toulouse University Hospital; Francia. Université Paul Sabatier; Franci

Falls in ambulatory non-demented patients with Parkinson's disease

This study aimed at determining the prevalence of falling in PD patients, to assess generic and disease-specific clinical and pharmacological factors, relationship with health-related quality of life (HR-QoL) and changes in falls from OFF to ON in patients with motor fluctuations. Six-hundred and eighty-three PD patients of the COPARK survey were evaluated (11 had missing data and were excluded from the analysis). Patients with falls were identified as those with a UPDRS Item 13 ¡Ý 1 in the ON condition. All patients were assessed in a standardized manner [demographics, treatments, Unified PD Rating Scale (UPDRS), Hospital Anxiety and Depression Scale, Pittsburg questionnaire and HR-QoL scales (SF36, PDQ39)]. Falling was reported by 108/672 (16 %) PD patients during the ON state and prevalence increased according to PD severity, from 5 % in Hoehn and Yahr stage 1-60 % in stage 4. Falling was significantly related to lower HR-QoL. Falling correlated with (1) generic factors such as female gender, age at the end of academic studies and diuretics consumption, (2) motor PD-specific factors including disease severity, frozen gait, difficulties when arising from a chair, dyskinesia and higher levodopa daily equivalent dose and (3) non-motor PD-specific factors such as orthostatic hypotension and hallucinations. Falling was more frequent in OFF than in ON in 48/74 (64 %) patients with motor fluctuations and remained unchanged in 27 patients (36 %). In summary, falling affected a significant proportion of PD patients, especially in advanced stages. It was associated with a variety of generic and PD-specific factors and was related to reduced HR-QoL.Fil: Rascol, Olivier. NS-Park Network; Francia. Université Paul Sabatier; Francia. Inserm; FranciaFil: Pérez Lloret, Santiago. Université Paul Sabatier; Francia. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: Damier, Philippe. Hôpital Laënnec; Francia. NS-Park Network; Francia. Inserm; FranciaFil: Delval, Arnaud. Seul Centre Hospitalier Universitaire; FranciaFil: Derkinderen, Pascal. Hôpital Laënnec; FranciaFil: Destée, Alain. NS-Park Network; Francia. Inserm; Francia. Seul Centre Hospitalier Universitaire; FranciaFil: Meissner, Wassilios G.. Universite de Bordeaux; Francia. Institut des Maladies Neurodégénératives; Francia. Centre Hospitalier Universitaire de Bordeaux; FranciaFil: Tison, Francois. Universite de Bordeaux; Francia. Centre Hospitalier Universitaire de Bordeaux; Francia. Institut des Maladies Neurodégénératives; Francia. NS-Park Network; FranciaFil: Negre Pages, Laurence. Inserm; Francia. NS-Park Network; Franci

Ciliary Beating Recovery in Deficient Human Airway Epithelial Cells after Lentivirus Ex Vivo Gene Therapy

Primary Ciliary Dyskinesia is a heterogeneous genetic disease that is characterized by cilia dysfunction of the epithelial cells lining the respiratory tracts, resulting in recurrent respiratory tract infections. Despite lifelong physiological therapy and antibiotics, the lungs of affected patients are progressively destroyed, leading to respiratory insufficiency. Recessive mutations in Dynein Axonemal Intermediate chain type 1 (DNAI1) gene have been described in 10% of cases of Primary Ciliary Dyskinesia. Our goal was to restore normal ciliary beating in DNAI1–deficient human airway epithelial cells. A lentiviral vector based on Simian Immunodeficiency Virus pseudotyped with Vesicular Stomatitis Virus Glycoprotein was used to transduce cultured human airway epithelial cells with a cDNA of DNAI1 driven by the Elongation Factor 1 promoter. Transcription and translation of the transduced gene were tested by RT–PCR and western blot, respectively. Human airway epithelial cells that were DNAI1–deficient due to compound heterozygous mutations, and consequently had immotile cilia and no outer dynein arm, were transduced by the lentivirus. Cilia beating was recorded and electron microscopy of the cilia was performed. Transcription and translation of the transduced DNAI1 gene were detected in human cells treated with the lentivirus. In addition, immotile cilia recovered a normal beat and outer dynein arms reappeared. We demonstrated that it is possible to obtain a normalization of ciliary beat frequency of deficient human airway epithelial cells by using a lentivirus to transduce cells with the therapeutic gene. This preliminary step constitutes a conceptual proof that is indispensable in the perspective of Primary Ciliary Dyskinesia's in vivo gene therapy. This is the first time that recovery of cilia beating is demonstrated in this disease

Agroecological practices supporting food production and reducing food insecurity in developing countries: A study on scientific literature in 17 countries

This report represents the initial effort to structure existing knowledge about agroecology as farming and food system in support of the EC Knowledge Centre on Food and Nutrition Security (https://ec.europa.eu/knowledge4policy/global-food-nutrition-security_en). Scientific literature has been screened on a selection of developing countries in which food and nutrition security and sustainable agriculture represent a focal sector for EU intervention (Benin, Burkina Faso, Cuba, Ethiopia, Ghana, Guatemala, Kenya, Lao PDR, Madagascar, Malawi, Mali, Nicaragua, Niger, Senegal, Tanzania, Togo, Zimbabwe). In total, 172 documents hae been synthesised, a country brief has been prepared for each investigated country, and some general conclusions have been drawn. Overall, the majority of analysed publications focuses on small scale, extensive farming systems that produce food at subsistence levels and for sale on local markets. This includes smallholder farming producing staple crops (millet, sorghum, yam, etc.) or vegetable gardening for local markets. The agroecological practices that are mostly studied are: agroforestry, intercropping (or mixed cropping), introduction of legumes in rotations, soil and water conservation practices (mulching, return of crop residues, zaï holes etc.), use of animal manure, biocontrol methods to mitigate chemical pesticide use. At least 50% of the analysed papers report a positive contribution of agroecological practices to food security, mostly due to improved yields and/or a better economic situation of producers. The improvement of soil quality is key to improve yields and consequently income and food security; this can be achieved using various practices including the use of residue mulch from tree leaves in agroforestry, as well as crop mixtures or intercropping and longer more diversified crop rotations. Moreover, higher on-farm crop species diversity often results in more diversified diets. Diversified crop systems, including the introduction of agroforestry, improves household nutritional status and has positive links to better health conditions. On the other hand, the lack of access to inputs is an important limitation to the improvement of soil fertility (manure, mineral fertilizer, leaf litter, etc.), which remains a major hindrance for food security. The agroecological cultivation of cash crops, post-harvest practices and crop-livestock integration were overall lacking in the scientific analyses, as well as the assessments of fully agroecological systems compared to individual agroecological practices or groups of practices. The report identifies three factors required for the substantial development of agroecology at the farm level: more financial support from the government, greater scientific knowledge on novel agroecological practices, and a higher market value for agroecological products.JRC.D.5-Food Securit

Vers la thérapie génique des hémoglobinopathies (sécurité oncogénique, et expansion cellulaire in vivo)

PARIS7-Bibliothèque centrale (751132105) / SudocSudocFranceF

Context-Aware Recommender Systems: Aggregation-Based Dimensionality Reduction

International audienceContext-aware recommender systems (CARS) rest on a multidimensional rating function: Users × Items × Context → Ratings. This multidimensional modeling should improve the quality of the recommendation process, but unfortunately, it is rare or even impossible to have ratings for all possible cases of context. Our objective is therefore twofold: (i) to reduce the dimensionality of the contextual information (in order to reduce the sparsity), which leads to (ii) propose a technique for aggregating the ratings associated with the aggregated dimensions. To do this, we organize, in the CARS utility matrix, the contextual informationaccording to hierarchical dimensions as is done in OLAP (OnLine Analytical Processing) and we use a regression-based approach for the rating aggregation according to previously defined hierarchies. Our approach supports multiple dimensions and hierarchical aggregation of ratings. It was validated on two real-world datasets

Problème de démarrage à froid pour les systèmes de recommandation dans les entrepôts de données

National audienceL’exploration OLAP (On-Line Analytical Processing) de données est un processus incrémental basé sur des requêtes effectuant une recherche d’informations dans un Entrepôt de Données (ED) multidimensionnelles. Afin de faciliter l’exploration d’un décideur, des systèmes de recommandation ont été proposés. Toutefois, lors de l’utilisation d’un nouveau système, ces systèmes de recommandations ne fonctionnent plus (problème de démarrage à froid). Dans cet article, nous proposons des recommandations pour un décideur qui est confronté à un problème de démarrage à froid. Notre processus est composé de quatre étapes : identifier le squelette des requêtes OLAP, prévoir des opérations candidates, calculer des recommandations sur les opérations candidates et classer ces recommandations

Contribution à l'étude du Syndrome de dépérissement du Ouistiti (comparaison avec la Maladie cœliaque humaine)

Le Syndrome de dépérissement du Ouistiti (WMS) est une pathologie majeure des callithricidés dont l'étiologie est incertaine. Ses manifestations sont principalement perte de poids, faiblesse et apathie. Une modification de la paroi intestinale aboutissant à une abrasion des villosités peut être observée. Histologiquement, on décèle souvent un phénomène inflammatoire diffus. Ces observations sont proches de celles faites sur des prélèvements de tubes digestifs de patients humains atteints de Maladie Cœliaque (MC) et un rapprochement de ces deux entités est envisageable. Une comparaison histologique de 35 prélèvements intestinaux concernant 9 individus morts du WMS avec des lésions observées chez les patients humains atteints de MC n'a mis en évidence qu'un cas dont les modifications peuvent être dues à un phénomène semblable. Un essai d'application du dépistage sérologique de la Maladie Cœliaque sur des sérums de 13 individus atteints du WMS n'a donné que des résultats négatifs.TOULOUSE3-BU Santé-Centrale (315552105) / SudocTOULOUSE-EN Vétérinaire (315552301) / SudocSudocFranceF