Characterization of the dry bean polygalacturonase-inhibiting protein (PGIP) gene family during Sclerotinia sclerotiorum (Sclerotiniaceae) infection.

Polygalacturonase-inhibiting proteins are leucine-rich repeat proteins that inhibit fungal endopolygalacturonases. The interaction of polygalacturonase-inhibiting protein with endopolygalacturonases limits the destructive potential of endopolygalacturonases and may trigger plant defense responses induced by oligogalacturonides. We examined the expression of fungal pg and plant Pvpgip genes in bean (Phaseolus vulgaris) stems infected with Sclerotinia sclerotiorum to determine whether any of them are associated with the infection process. Transcriptional analysis was carried out by means of semi-quantitative reverse transcription PCR or real-time PCR. The sspg1 gene was highly expressed during infection; sspg3 was regulated during the later phases of infection; sspg5 was more uniformly expressed during infection, whereas sspg6 was only weakly expressed. During the course of infection, Pvpgip1 transcripts were not detected at early stages, but they appeared 72 h post-inoculation. High levels of Pvpgip2 expression were observed during the initial phase of infection; the transcript peaked by 48 h post-inoculation and declined by 72 h post-inoculation. Pvpgip3 expression increased strongly at 96 h post-inoculation. Pvpgip4 was constantly present from 24 h post-inoculation until the end of the experiment. However, we detected higher levels of the Pvpgip4 transcript in the necrotic lesion area than in plants that had been mechanically wounded. Remarkably, only Pvpgip4 appeared to be moderately induced by mechanical wounding. These results provide evidence that endopolygalacturonases contribute to the infection process during host colonization by promoting the release of plant cell oligogalacturonides, which are powerful signaling molecules and may also activate plant defenses, such as polygalacturonase-inhibiting proteins

Classificacao de sitio para povoamento de Pinus taeda no sul do Brasil.

Edição dos Anais do Congresso Florestal Brasileiro, 6., 1990, Campos do Jordão

Construção de um produto terminológico da Rede Agro-Hidro: etapa 5 (gerenciamento da base de dados terminológicos).

A Empresa Brasileira de Pesquisa Agropecuária (Embrapa) deu início ao projeto Rede AgroHidro - Agricultura e Recursos Hídricos nos Biomas Brasileiros, que tem como objetivo pensar em estratégias de gestão, comunicação e capacitação, a fim de desenvolver informações que ajudem a sociedade brasileira a lidar com essa questão

Ising Ferromagnet: Zero-Temperature Dynamic Evolution

The dynamic evolution at zero temperature of a uniform Ising ferromagnet on a square lattice is followed by Monte Carlo computer simulations. The system always eventually reaches a final, absorbing state, which sometimes coincides with a ground state (all spins parallel), and sometimes does not (parallel stripes of spins up and down). We initiate here the numerical study of ``Chaotic Time Dependence'' (CTD) by seeing how much information about the final state is predictable from the randomly generated quenched initial state. CTD was originally proposed to explain how nonequilibrium spin glasses could manifest equilibrium pure state structure, but in simpler systems such as homogeneous ferromagnets it is closely related to long-term predictability and our results suggest that CTD might indeed occur in the infinite volume limit.Comment: 14 pages, Latex with 8 EPS figure

Embedded Star Formation in the Eagle Nebula with Spitzer/GLIMPSE

We present new Spitzer photometry of the Eagle Nebula (M16, containing the optical cluster NGC 6611) combined with near-infrared photometry from 2MASS. We use dust radiative transfer models, mid-infrared and near-infrared color-color analysis, and mid-infrared spectral indices to analyze point source spectral energy distributions, select candidate young stellar objects (YSOs), and constrain their mass and evolutionary state. Comparison of the different protostellar selection methods shows that mid-infrared methods are consistent, but as has been known for some time, near-infrared-only analysis misses some young objects. We reveal more than 400 protostellar candidates, including one massive young stellar object (YSO) that has not been previously highlighted. The YSO distribution supports a picture of distributed low-level star formation, with no strong evidence of triggered star formation in the ``pillars''. We confirm the youth of NGC 6611 by a large fraction of infrared-excess sources, and reveal a younger cluster of YSOs in the nearby molecular cloud. Analysis of the YSO clustering properties shows a possible imprint of the molecular cloud's Jeans length. Multiwavelength mid-IR imaging thus allows us to analyze the protostellar population, to measure the dust temperature and column density, and to relate these in a consistent picture of star formation in M16.Comment: 16p preprint - ApJ accepte