Isolation And Characterization Of Microsatellite Markers From The Stingless Bee Nannotrigona Testaceicornis

Conservation of natural populations and handling of breeding programs would benefit from the availability of molecular markers. Stingless bees are one of the most important pollinators in several ecosystems. Thus, seventeen microsatellite markers were developed from an enriched genomic library of Nannotrigona testaceicornis. They were characterized using 50 samples. The expected and observed heterozygosities ranged from 0.59 to 0.89 and from 0.39 to 0.79, respectively. These markers will contribute to advance researches on the genetic conservation, characterization and preservation of the Brazilian native bees. © Springer Science+Business Media B.V. 2009.119799Billotte, N., Lagoda, P.J.L., Risterucci, A.M., Baurens, F.C., Microsatellite-enriched libraries: Applied methodology for the development of SSR markers in tropical crops (1999) Fruits, 54, pp. 277-288Creste, S., Tulmann-Neto, A., Figueira, A., Detection of single sequence repeat polymorphism in denaturing polyacrylamide sequencing gels by silver staining (2001) Plant Mol Biol Report, 19, pp. 1-8Francisco, F.O., Silvestre, D., Arias, M.C., Mitochondrial DNA characterization of five species of Plebeia (Apidae: Meliponini): RFLP and restriction map (2001) Apidologie, 32, pp. 323-332Green, C.L., Franck, C., Oldroyd, B.P., Characterization of microsatellite loci for Trigona carbonaria, a stingless bee endemic to Australia (2001) Mol Ecol Notes, 1, pp. 89-92Kerr, W.E., Carvalho, G.A., Nascimento, V.A., (1996) Abelha Uruçu, , Biologia, manejo e conservação, Fundação Acangaú, Belo HorizonteNei, M., Estimation of average heterozygosity and genetic distance from a small number of individuals (1978) Genetics, 89, pp. 583-590Paxton, R.J., Weißschuh, N., Quezada-Euan, J.J.G., Characterization of dinucleotide microsatellite loci for stingless bees (1999) Mol Ecol, 8, pp. 685-702Peters, J.M., Queller, D.C., Imperatriz-Fonseca, V.L., Strassmann, J.E., Microsatellite loci for stingless bees (1998) Mol Ecol, 7, pp. 783-792Raymond, M., Rousset, F., GENEPOP (version 1.2): Population genetics software for exact tests and ecumenicism (1995) J Hered, 86, pp. 248-249Rozen, S., Skaletsky, H.J., PRIMER 3 on the WWW for general users and for biologist programmers (2000) Bioinformatics Methods and Protocols: Methods in Molecular Biology, pp. 365-386. , In: Krawetz S, Misener S (eds), Humana Press, Totowa, NJSambrook, J., Fritsch, E.F., Maniatis, T., (1989) Molecular Cloning: A Laboratory Manual, , 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NYvan Oosterhout, C., Hutchinson, W.F., Wills, D.P.M., Shipley, P., MICRO-CHECKER: Software for identifying and correcting genotyping errors in microsatellite data (2004) Mol Ecol Notes, 4, pp. 535-53