Influência de rotações de culturas nas propriedades físico-hídricas de um Latossolo Vermelho em plantio direto.

A adoção do plantio direto, com a inclusão de espécies gramíneas de relação C/N alta, poderá minimizar a degradação estrutural do solo. O objetivo deste trabalho foi avaliar a influência da inclusão da braquiária na rotação de culturas nas propriedades físico-hídricas e no conteúdo de matéria orgânica de um Latossolo Vermelho distrófico em plantio direto. O delineamento experimental foi o de blocos ao acaso, em parcelas subdivididas, com quatro repetições. As parcelas foram compostas por quatro sistemas de rotação, e as subparcelas, por quatro profundidades de amostragem (0-10; 10-20; 20-40, e 40-60 cm). As culturas utilizadas foram: arroz (A); feijão irrigado (F); milho (M); soja (S), e arroz (A+B), milho (M+B) e soja (S+B) consorciados com braquiária. Os sistemas de rotação foram: S1 - A/F/M/F/S/F; S2 - A+B/F/M+B/F/S+B/F; S3 - M/F/S/F/S/F, e S4 - M+B/F/S+B/F/S+B/F. Uma área de mata nativa, situada a 500 m do experimento, foi considerada como controle. A inclusão da braquiária na rotação de culturas não afetou a densidade, a macroporosidade e a capacidade de água disponível do solo. A porosidade total e o teor de matéria orgânica do solo foram favorecidos pelas rotações que incluíam maior número de cultivos de soja. A associação de soja com a braquiária contribuiu para a estabilidade dos agregados, e a de gramíneas de verão com a braquiária, para a diminuição da resistência do solo à penetração

Doses e formas de aplicação da adubação potássica na rotação soja, milheto e algodão em sistema plantio direto.

Este estudo teve como objetivo avaliar a eficiência da adubação potássica, com relação às doses, modos (sulco, a lanço e parcelada) e épocas de aplicação (pré-semeadura, semeadura e cobertura), na sucessão de culturas soja-milheto-algodoeiro, cultivadas em sistema plantio direto, em Latossolo Vermelho, no município de Turvelândia, Goiás (17o51?S, 50o18?W). O delineamento experimental adotado foi o de blocos casualizados, com quatro repetições, em esquema fatorial. A fonte de potássio utilizada nas adubações foi o cloreto de potássio. Na soja, os tratamentos utilizados foram doses de K2O (0 kg ha-1, 30 kg ha-1, 60 kg ha-1 e 180 kg ha-1), aplicadas em pré-semeadura (a lanço) e na semeadura (no sulco), com e sem cobertura. Na cultura do algodoeiro, os tratamentos foram doses de K2O (0 kg ha-1, 60 kg ha-1, 120 kg ha-1 e 240 kg ha-1), aplicadas em pré-semeadura (a lanço) e na semeadura (no sulco), com 0, 1 ou 2 coberturas. A adubação em pré-semeadura foi realizada no milheto. Não houve efeito da adubação potássica sobre a produtividade da cultura da soja. O milheto, como cobertura do solo, aproveitou, mais eficientemente, a dose de 60 kg ha-1 de K2O, aplicada na cultura da soja. Para a cultura do algodoeiro, a maior eficiência agronômica foi obtida com dose de 146 kg ha-1 de K2O, em pré-semeadura. Os resultados indicaram, também, que houve efeito positivo da adubação potássica sobre a qualidade da fibra do algodão

FRD characterization in large-scale for FOCCoS of Prime Focus Spectrograph for Subaru telescope

The focal ratio degradation effects on optical fibers, technically referred to as FRD, has been the subject of intense studies since the beginning of the use of optical fibers in the construction of instruments applied in astronomy. A number of studies attempt to relate FRD to light loss in the optical system and other studies attempt to qualify and quantify FRD as a function of the stress induced during assembly of the structures supporting the ends of the optical fibers. In this work, we present a large-scale study to characterize FRD in all the fibers that make up the cables of the FOCCoS, Fiber Optical Cable and Connectors System project. FOCCoS, has the main function of capturing the direct light from the focal plane of Subaru Telescope using 2400 optical fibers, each one with a microlens in its tip, and conducting this light through a route containing connectors to a set of four spectrographs. The optical fiber cable is divided in 3 different segments called Cable A, Cable B and Cable C. Multi-fibers connectors assure precise connection among all optical fibers of the segments, providing flexibility for instrument changes. Our study provides procedures and methods to analyze the effects of FRD on all cable segments for each type of termination involved. Special attention is devoted to the understanding of how angular deviations between the input surface of the fiber and the test beam can significantly influence the calculation of FRD in optical fibers

Bothrops jararaca Peptide with Anti-Hypertensive Action Normalizes Endothelium Dysfunction Involved in Physiopathology of Preeclampsia

Preeclampsia, a pregnancy-specific syndrome characterized by hypertension, proteinuria and edema, is a major cause of fetal and maternal morbidity and mortality especially in developing countries. Bj-PRO-10c, a proline-rich peptide isolated from Bothrops jararaca venom, has been attributed with potent anti-hypertensive effects. Recently, we have shown that Bj-PRO-10c-induced anti-hypertensive actions involved NO production in spontaneous hypertensive rats. Using in vitro studies we now show that Bj-PRO-10c was able to increase NO production in human umbilical vein endothelial cells from hypertensive pregnant women (HUVEC-PE) to levels observed in HUVEC of normotensive women. Moreover, in the presence of the peptide, eNOS expression as well as argininosuccinate synthase activity, the key rate-limiting enzyme of the citrulline-NO cycle, were enhanced. In addition, excessive superoxide production due to NO deficiency, one of the major deleterious effects of the disease, was inhibited by Bj-PRO-10c. Bj-PRO-10c induced intracellular calcium fluxes in both, HUVEC-PE and HUVEC, which, however, led to activation of eNOS expression only in HUVEC-PE. Since Bj-PRO-10c promoted biological effects in HUVEC from patients suffering from the disorder and not in normotensive pregnant women, we hypothesize that Bj-PRO-10c induces its anti-hypertensive effect in mothers with preeclampsia. Such properties may initiate the development of novel therapeutics for treating preeclampsia

Bothrops jararaca Peptide with Anti-Hypertensive Action Normalizes Endothelium Dysfunction Involved in Physiopathology of Preeclampsia

Preeclampsia, a pregnancy-specific syndrome characterized by hypertension, proteinuria and edema, is a major cause of fetal and maternal morbidity and mortality especially in developing countries. Bj-PRO-10c, a proline-rich peptide isolated from Bothrops jararaca venom, has been attributed with potent anti-hypertensive effects. Recently, we have shown that Bj-PRO-10c-induced anti-hypertensive actions involved NO production in spontaneous hypertensive rats. Using in vitro studies we now show that Bj-PRO-10c was able to increase NO production in human umbilical vein endothelial cells from hypertensive pregnant women (HUVEC-PE) to levels observed in HUVEC of normotensive women. Moreover, in the presence of the peptide, eNOS expression as well as argininosuccinate synthase activity, the key rate-limiting enzyme of the citrulline-NO cycle, were enhanced. In addition, excessive superoxide production due to NO deficiency, one of the major deleterious effects of the disease, was inhibited by Bj-PRO-10c. Bj-PRO-10c induced intracellular calcium fluxes in both, HUVEC-PE and HUVEC, which, however, led to activation of eNOS expression only in HUVEC-PE. Since Bj-PRO-10c promoted biological effects in HUVEC from patients suffering from the disorder and not in normotensive pregnant women, we hypothesize that Bj-PRO-10c induces its anti-hypertensive effect in mothers with preeclampsia. Such properties may initiate the development of novel therapeutics for treating preeclampsia

Genotoxic and Cytotoxic Safety Evaluation of Papain (Carica papaya L.) Using In Vitro Assays

Papain, a phytotherapeutic agent, has been used in the treatment of eschars and as a debriding chemical agent to remove damaged or necrotic tissue of pressure ulcers and gangrene. Its benefits in these treatments are deemed effective, since more than 5000 patients, at the public university hospital at Rio de Janeiro, Brazil, have undergone papain treatment and presented satisfactory results. Despite its extensive use, there is little information about toxic and mutagenic properties of papain. This work evaluated the toxic and mutagenic potential of papain and its potential antioxidant activity against induced-H2O2 oxidative stress in Escherichia coli strains. Cytotoxicity assay, Growth inhibition test, WP2-Mutoxitest and Plasmid-DNA treatment, and agarose gel electrophoresis were used to investigate if papain would present any toxic or mutagenic potential as well as if papain would display antioxidant properties. Papain exhibited negative results for all tests. This agent presented an activity protecting cells against H2O2-induced mutagenesis