Influence of repeated intravital extraction of eggs from sturgeon hybrids on their oogenesis under conditions of recirculation aquaculture system

Dynamics of cytomorphological characteristics of oocytes in ovaries is considered for two sturgeon hybrids: besters of the breed Burtlevskaya Huso huso (Linnaeus) x Acipenser ruthenus (Linnaeus) and the breed Aksayskaya Acipenser ruthenus x ( Huso huso x Acipenser ruthenus ) on the experimental data obtained under controlled conditions in the aquatic complex in Moscow (Russia) in 2010-2012. Methods of intravital eggs extraction, ultrasound diagnostics, biopsy with the probe, anesthesia and histological analysis are applied. Structure of membrane is similar for straight sturgeon species and hybrids, but the membrane thickness and the term of its emergence on certain maturity stage are somewhat different, so the eggs of bester could be distinguished by the oocyte membrane thickness. Mean interspawning interval for the hybrids in conditions of recirculation aquaculture system at water temperature 20-21 °C is determined as 10-12 months

Interpenetrated (8,3)-c and (10,3)-b Metal–Organic Frameworks Based on {Fe III 3 } and {Fe III 2 Co II } Pivalate Spin Clusters

MOFs composed of interpenetrated (8,3)-c and (10,3)-b coordination networks based on trinuclear Fe(III) and Fe(III)/Co(II) pivalate clusters and N-donor organic linkers were synthesized and characterize

Aberrant Methylation of 20 miRNA Genes Specifically Involved in Various Steps of Ovarian Carcinoma Spread: From Primary Tumors to Peritoneal Macroscopic Metastases

Our work aimed to differentiate 20 aberrantly methylated miRNA genes that participate at different stages of development and metastasis of ovarian carcinoma (OvCa) using methylation-specific qPCR in a representative set of clinical samples: 102 primary tumors without and with metastases (to lymph nodes, peritoneum, or distant organs) and 30 peritoneal macroscopic metastases (PMM). Thirteen miRNA genes (MIR107, MIR124-2, MIR124-3, MIR125B-1, MIR127, MIR129-2, MIR130B, MIR132, MIR193A, MIR339, MIR34B/C, MIR9-1, and MIR9-3) were hypermethylated already at the early stages of OvCa, while hypermethylation of MIR1258, MIR137, MIR203A, and MIR375 was pronounced in metastatic tumors, and MIR148A showed high methylation levels specifically in PMM. We confirmed the significant relationship between methylation and expression levels for 11 out of 12 miRNAs analyzed by qRT-PCR. Moreover, expression levels of six miRNAs were significantly decreased in metastatic tumors in comparison with nonmetastatic ones, and downregulation of miR-203a-3p was the most significant. We revealed an inverse relationship between expression levels of miR-203a-3p and those of ZEB1 and ZEB2 genes, which are EMT drivers. We also identified three miRNA genes (MIR148A, MIR9-1, and MIR193A) that likely regulate EMT–MET reversion in the colonization of PMM. According to the Kaplan–Meier analysis, hypermethylation of several examined miRNA genes was associated with poorer overall survival of OvCa patients, and high methylation levels of MIR130B and MIR9-1 were related to the greatest relative risk of death

Interpenetrated (8,3)‑c and (10,3)‑b Metal–Organic Frameworks Based on {Fe^III₃} and {Fe^III₂Co^II} Pivalate Spin Clusters

Two new magnetic cluster-based 3D coordination polymers consisting of μ₃-oxo-centered cationic homometallic [Fe^III₃O­(O₂CCMe₃)₆]⁺ or neutral heterometallic [Fe^III₂Co^IIO­(O₂CCMe₃)₆] coordination clusters bridged by different <i>N</i>,<i>N</i>′-donor ligands into three-dimensional networks of {[Fe₃O­(O₂CCMe₃)₆(4,4′-bpy)_1.5]­(OH)·0.75­(CH₂Cl₂)·<i>x</i>(H₂O)}_<i>n</i> (<b>1</b>, <i>x</i> ≈ 8) and {[Fe₂CoO­(O₂CCMe₃)₆(bpe)_0.5(pyz)]}_<i>n</i> (<b>2</b>) (where 4,4′-bpy = 4,4′-bipyridine; bpe = 1,2′-bis­(4-pyridyl)­ethylene; pyz = pyrazine) have been prepared under solvothermal conditions. Single-crystal X-ray diffraction studies reveal the existence of a 6-fold interpenetrated network with rare (8,3)-c (<b>etc</b>) topology for <b>1</b> and a 3-fold interpenetrated network with (10,3)-b (<b>ths</b>) topology for <b>2</b>. The interpenetration effectively results in very low BET surface areas, and the compounds have to be regarded as nonporous. Magnetic studies of <b>1</b> and <b>2</b> point to both ferro- and antiferromagnetic intra- and intercluster exchange interactions between the isotropic Fe^III and the strongly anisotropic Co^II spin centers. ⁵⁷Fe Mössbauer spectroscopy confirms the uniform ferric (+III) valence state in both <b>1</b> and <b>2</b>, and low-temperature data for <b>1</b> point toward distinct hyperfine fields for the Fe sites

Interpenetrated (8,3)‑c and (10,3)‑b Metal–Organic Frameworks Based on {Fe^III₃} and {Fe^III₂Co^II} Pivalate Spin Clusters

Two new magnetic cluster-based 3D coordination polymers consisting of μ₃-oxo-centered cationic homometallic [Fe^III₃O­(O₂CCMe₃)₆]⁺ or neutral heterometallic [Fe^III₂Co^IIO­(O₂CCMe₃)₆] coordination clusters bridged by different <i>N</i>,<i>N</i>′-donor ligands into three-dimensional networks of {[Fe₃O­(O₂CCMe₃)₆(4,4′-bpy)_1.5]­(OH)·0.75­(CH₂Cl₂)·<i>x</i>(H₂O)}_<i>n</i> (<b>1</b>, <i>x</i> ≈ 8) and {[Fe₂CoO­(O₂CCMe₃)₆(bpe)_0.5(pyz)]}_<i>n</i> (<b>2</b>) (where 4,4′-bpy = 4,4′-bipyridine; bpe = 1,2′-bis­(4-pyridyl)­ethylene; pyz = pyrazine) have been prepared under solvothermal conditions. Single-crystal X-ray diffraction studies reveal the existence of a 6-fold interpenetrated network with rare (8,3)-c (<b>etc</b>) topology for <b>1</b> and a 3-fold interpenetrated network with (10,3)-b (<b>ths</b>) topology for <b>2</b>. The interpenetration effectively results in very low BET surface areas, and the compounds have to be regarded as nonporous. Magnetic studies of <b>1</b> and <b>2</b> point to both ferro- and antiferromagnetic intra- and intercluster exchange interactions between the isotropic Fe^III and the strongly anisotropic Co^II spin centers. ⁵⁷Fe Mössbauer spectroscopy confirms the uniform ferric (+III) valence state in both <b>1</b> and <b>2</b>, and low-temperature data for <b>1</b> point toward distinct hyperfine fields for the Fe sites

Interpenetrated (8,3)‑c and (10,3)‑b Metal–Organic Frameworks Based on {Fe^III₃} and {Fe^III₂Co^II} Pivalate Spin Clusters

Two new magnetic cluster-based 3D coordination polymers consisting of μ₃-oxo-centered cationic homometallic [Fe^III₃O­(O₂CCMe₃)₆]⁺ or neutral heterometallic [Fe^III₂Co^IIO­(O₂CCMe₃)₆] coordination clusters bridged by different <i>N</i>,<i>N</i>′-donor ligands into three-dimensional networks of {[Fe₃O­(O₂CCMe₃)₆(4,4′-bpy)_1.5]­(OH)·0.75­(CH₂Cl₂)·<i>x</i>(H₂O)}_<i>n</i> (<b>1</b>, <i>x</i> ≈ 8) and {[Fe₂CoO­(O₂CCMe₃)₆(bpe)_0.5(pyz)]}_<i>n</i> (<b>2</b>) (where 4,4′-bpy = 4,4′-bipyridine; bpe = 1,2′-bis­(4-pyridyl)­ethylene; pyz = pyrazine) have been prepared under solvothermal conditions. Single-crystal X-ray diffraction studies reveal the existence of a 6-fold interpenetrated network with rare (8,3)-c (<b>etc</b>) topology for <b>1</b> and a 3-fold interpenetrated network with (10,3)-b (<b>ths</b>) topology for <b>2</b>. The interpenetration effectively results in very low BET surface areas, and the compounds have to be regarded as nonporous. Magnetic studies of <b>1</b> and <b>2</b> point to both ferro- and antiferromagnetic intra- and intercluster exchange interactions between the isotropic Fe^III and the strongly anisotropic Co^II spin centers. ⁵⁷Fe Mössbauer spectroscopy confirms the uniform ferric (+III) valence state in both <b>1</b> and <b>2</b>, and low-temperature data for <b>1</b> point toward distinct hyperfine fields for the Fe sites

"Flora of Russia" on iNaturalist: a dataset

The "Flora of Russia" project on iNaturalist brought together professional scientists and amateur naturalists from all over the country. Over 10,000 people are involved in the data collection.Within 20 months the participants accumulated over 750,000 photo observations of 6,853 species of the Russian flora. This constitutes the largest dataset of open spatial data on the country’s biodiversity and a leading source of data on the current state of the national flora. About 85% of all project data are available under free licenses (CC0, CC-BY, CC-BY-NC) and can be freely used in scientific, educational and environmental activities

"Flora of Russia" on iNaturalist: a dataset

The "Flora of Russia" project on iNaturalist brought together professional scientists and amateur naturalists from all over the country. Over 10,000 people are involved in the data collection.Within 20 months the participants accumulated over 750,000 photo observations of 6,853 species of the Russian flora. This constitutes the largest dataset of open spatial data on the country’s biodiversity and a leading source of data on the current state of the national flora. About 85% of all project data are available under free licenses (CC0, CC-BY, CC-BY-NC) and can be freely used in scientific, educational and environmental activities