The waking brain: an update

Wakefulness and consciousness depend on perturbation of the cortical soliloquy. Ascending activation of the cerebral cortex is characteristic for both waking and paradoxical (REM) sleep. These evolutionary conserved activating systems build a network in the brainstem, midbrain, and diencephalon that contains the neurotransmitters and neuromodulators glutamate, histamine, acetylcholine, the catecholamines, serotonin, and some neuropeptides orchestrating the different behavioral states. Inhibition of these waking systems by GABAergic neurons allows sleep. Over the past decades, a prominent role became evident for the histaminergic and the orexinergic neurons as a hypothalamic waking center

Examination of investment proposals for their inclusion in the infrastructure programs of the energy industry

The paper discusses the key issues that allow finding approaches to solving the problem of investment activity in the process of developing and implementing investment projects and construction programs in the real sector of the economy. The mechanism of formation of investment programs is considered. The process of examination of investment projects for their inclusion in the investment program has been determined. The algorithm for making investment decisions is proposed

Intersubjective management in aerospace engineering

This article presents a postnonclassical approach to create the science of management processes organization in a developing society, the focus of which is “the man of culture”, i.e. the man, not just adhering to cultural norms, but also creating new concepts and products of culture. This science is proposed to be called Evergetics. The purpose of the study is the analysis science of management processes organization in a developing aerospace engineering and other industrial areas of society. The authors describe the main aspects and procedures evergetics management in aerospace engineering. They uses the comparison method, compares classical and modern approaches and technologies of management. In evergetics management model each member of society or organization is interested in augmenting his cultural heritage he is producing, which entails a raise of stability in process of engineering actions and a raise cultural potential of the society as a whole and, as a consequence, an increase in the proportion of moral and ethical managerial decisions and corresponding to them benevolent actions in organizational life. Summarize the article’s main findings, authors may in some main conclusions about necessity evergetics model and intersubjective technologies in the creation and development of aerospace engineering

Intersubjective management in aerospace engineering

This article presents a postnonclassical approach to create the science of management processes organization in a developing society, the focus of which is “the man of culture”, i.e. the man, not just adhering to cultural norms, but also creating new concepts and products of culture. This science is proposed to be called Evergetics. The purpose of the study is the analysis science of management processes organization in a developing aerospace engineering and other industrial areas of society. The authors describe the main aspects and procedures evergetics management in aerospace engineering. They uses the comparison method, compares classical and modern approaches and technologies of management. In evergetics management model each member of society or organization is interested in augmenting his cultural heritage he is producing, which entails a raise of stability in process of engineering actions and a raise cultural potential of the society as a whole and, as a consequence, an increase in the proportion of moral and ethical managerial decisions and corresponding to them benevolent actions in organizational life. Summarize the article’s main findings, authors may in some main conclusions about necessity evergetics model and intersubjective technologies in the creation and development of aerospace engineering

A new simple method for quantification and locating P and N reserves in microalgal cells based on energy-filtered transmission electron microscopy (EFTEM) elemental maps.

We established a new simple approach to study phosphorus (P) and nitrogen (N) reserves at subcellular level potentially applicable to various types of cells capable of accumulating P- and/or N-rich inclusions. Here, we report on using this approach for locating and assessing the abundance of the P and N reserves in microalgal and cyanobacterial cells. The approach includes separation of the signal from P- or N-rich structures from noise on the energy-filtered transmission electron microscopy (EFTEM) P- or N-maps. The separation includes (i) relative entropy estimation for each pixel of the map, (ii) binary thresholding of the map, and (iii) segmenting the image to assess the inclusion relative area and localization in the cell section. The separation is based on comparing the a posteriori probability that a pixel of the map contains information about the sample vs. Gaussian a priori probability that the pixel contains noise. The difference is expressed as relative entropy value for the pixel; positive values are characteristic of the pixels containing the payload information about the sample. This is the first known method for quantification and locating at a subcellular level P-rich and N-rich inclusions including tiny (< 180 nm) structures. We demonstrated the applicability of the proposed method both to the cells of eukaryotic green microalgae and cyanobacteria. Using the new method, we elucidated the heterogeneity of the studied cells in accumulation of P and N reserves across different species. The proposed approach will be handy for any cytological and microbiological study requiring a comparative assessment of subcellular distribution of cyanophycin, polyphosphates or other type of P- or N-rich inclusions. An added value is the potential of this approach for automation of the data processing and evaluation enabling an unprecedented increase of the EFTEM analysis throughput

Applying the competence-based approach to management in the aerospace industry

Problems of management in aerospace manufacturing are similar to those we observe in other sectors, the main of which is the flattening of strategic management. The main reason lies in the attitude towards human resource of the organization. In the aerospace industry employs 250 thousand people, who need individual approach. The individual approach can offer competence-based approach to management. The purpose of the study is proof of the benefits of the competency approach to human resource management in context strategic management of the aerospace organization. To achieve this goal it is possible to obtain the method of comparative analysis. The article compares two approaches to personnel management. The transition to competence-based human resource management means (a) a different understanding of the object of management; (b) involvement in all functions of human resource management «knowledge – skills – abilities» of the employee; (c) to change the approach to strategic management aerospace industry

Applying the competence-based approach to management in the aerospace industry

Problems of management in aerospace manufacturing are similar to those we observe in other sectors, the main of which is the flattening of strategic management. The main reason lies in the attitude towards human resource of the organization. In the aerospace industry employs 250 thousand people, who need individual approach. The individual approach can offer competence-based approach to management. The purpose of the study is proof of the benefits of the competency approach to human resource management in context strategic management of the aerospace organization. To achieve this goal it is possible to obtain the method of comparative analysis. The article compares two approaches to personnel management. The transition to competence-based human resource management means (a) a different understanding of the object of management; (b) involvement in all functions of human resource management «knowledge – skills – abilities» of the employee; (c) to change the approach to strategic management aerospace industry

Light absorption and scattering by high light-tolerant, fast-growing Chlorella vulgaris IPPAS C-1 cells

Algal cells are highly complex optical systems that can dynamically change their structure. Consequently, absorption and scattering properties of algae change, while the cells are acclimating to different light conditions or during growth and division in a cell cycle. This may be particularly important in algal species that can grow rapidly under very high-light such as Chlorella vulgaris IPPAS C-1 that is studied here. From cell transmittance measured conventionally and using integrating sphere, we evaluated absorption and scattering coefficients and cross sections per cell dry weight and chlorophyll content. This was done for asynchronous cell culture grown in low-light (LL; 220 μmol (photons) m−2 s−1) or high-light (HL; 1760 μmol (photons) m−2 s−1) light, as well as during cell cycle of synchronous culture grown in HL. During the cell cycle, we also determined cell ultrastructural organization by transmission electron microscopy, and correlated its parameters with absorption and scattering cross sections per cell dry weight. We found that the IPPAS C-1 cells of asynchronous culture scatter light more than other cells, however, internal organization of the cells that is decisive for scattering is less sensitive to HL and LL treatment than the cell pigment content that controls absorption. The light scattering and absorption were dynamically changed during cell cycle of synchronous cells grown in the HL. Changes in ratio of chloroplast to protoplast area, reflecting amount of scattering chloroplast membrane (outer, inner) interfaces, best correlated with changes in light scattering. We suggest that the increased light scattering by the HL-acclimated IPPAS C-1 cells might be responsible for increased HL resilience reported in the literature. Biotechnological aspect of this study is that the scattering and absorption properties of phytoplankton cells ought to be calibrated for each particular growth phase or irradiance to which the cells are acclimated

Sunscreen Effect Exerted by Secondary Carotenoids and Mycosporine-like Amino Acids in the Aeroterrestrial Chlorophyte <i>Coelastrella rubescens</i> under High Light and UV-A Irradiation

The microalga Coelastrella rubescens dwells in habitats with excessive solar irradiation; consequently, it must accumulate diverse compounds to protect itself. We characterized the array of photoprotective compounds in C. rubescens. Toward this goal, we exposed the cells to high fluxes of visible light and UV-A and analyzed the ability of hydrophilic and hydrophobic extracts from the cells to absorb radiation. Potential light-screening compounds were profiled by thin layer chromatography and UPLC-MS. Coelastrella accumulated diverse carotenoids that absorbed visible light in the blue–green part of the spectrum and mycosporine-like amino acids (MAA) that absorbed the UV-A. It is the first report on the occurrence of MAA in Coelastrella. Two new MAA, named coelastrin A and coelastrin B, were identified. Transmission electron microscopy revealed the development of hydrophobic subcompartments under the high light and UV-A exposition. We also evaluate and discuss sporopollenin-like compounds in the cell wall and autophagy-like processes as the possible reason for the decrease in sunlight absorption by cells, in addition to inducible sunscreen accumulation. The results suggested that C. rubescens NAMSU R1 accumulates a broad range of valuable photoprotective compounds in response to UV-A and visible light irradiation, which indicates this strain as a potential producer for biotechnology