Histotopographic and morphometric characteristics of the oral mucosa postoperative wounds healing, depending on the method of connecting the wound edges

Implementation of high-quality tissue connections and hemostasis in oral surgery is an urgent problem of modern dentistry, due to the lack of available universal methods that would help to facilitate the work of oral surgeon and reduce wound healing time. The aim of the research. To investigate and study the regeneration processes in the oral mucosa after high-frequency electric welding, suturing and medical adhesive composition. Materials and methods. This study compared the effect of three connection methods and hemostasis: high-frequency electric welding (EKVZ-300M1 “PATONMED®”, Ukraine), suture method (nylon 5/0 “PIRUS®”, China), adhesive composition (“Histoacryl®”, Germany). 72 rabbits were involved in experiment. An incision of the soft tissues of the vestibulum of the oral cavity of the maxilla with a length of 1.3–1.5 cm was made. On days 3, 7, 14 and 21, tissue samples were excised, histological and morphometric examination of the material were performed. Results. By statistical data processing of 72 rabbits was found that the reliability of differences in the levels of morphometric parameters between groups on each day is very high, as evidenced by the calculated values ​​of the U-test. In 38 cases (90.5 %), the significance level was p≤0.01, in the other 4 cases – p≤0.05. The lowest value level of neutrophilic granulocytes was in rabbits of group No.1 (electric welding). In rabbits of group No. 1 GFBC were not observed in the 8 fields of view throughout the experiment. Conclusions. High-frequency electric welding of soft tissues can be used for effective intraoperative hemostasis and connection of the intraoral wound edges. Due to the absence of a foreign body (adhesive masses, filaments) and moderate alterative effect, histological examination indicates accelerated activation of regenerative processes in the early stages of observation in rabbits of group No. 1

NHS procurement and the origins of the personal protective equipment crisis

COVID-19 has thrown NHS procurement into the limelight, but the shortage of personal protective equipment (PPE) has complex origins. This article explores the long-lasting struggle for centralisation in NHS procurement and its impact on the current PPE crisis

Interpersonal relations in the teaching staff and pro-environmental behavior of teachers of general educational organizations

The article is devoted to the study of the need for communication, which is the basis of cooperation and the relationship of people with each other. The main question in understanding of the nature of interpersonal relationships is the question of their connection with social relations. This article presents the results of the study of interpersonal relations in the teaching staff and pro-environmental behavior of teashers. The research methods contain a generalization of the best psychological experience in the study of this problem, description, testing, the method of logical generalizations, methods of qualitative and quantitative data analysis. The hypothesis of the relationship between the level of anxiety and the preferred style of interpersonal relationships among teachers is presented. The results obtained in the study can be used to improve the work of teaching collectives, for family and personal counselingб for further research in the field of team management

Revisiting patient expectations and experiences of antibiotics in an era of antimicrobial resistance: Qualitative study.

OBJECTIVE: To investigate contemporary patient expectations and experiences of antibiotic prescribing in England. BACKGROUND: Primary care providers' compliance with patient influences has been identified as a motivation for antibiotic-prescribing behaviour. Since 2013, there have been concerted efforts to publicize and address the growing threat of antimicrobial resistance. A fresh qualitative insight into patient expectations and experiences is needed. DESIGN: Qualitative study using semi-structured interviews. SETTING AND PARTICIPANTS: Two English regions, one an urban metropolitan area and the other a town in rural England. Patients who recently consulted for infections were recruited. The information power approach was used to determine the number of participants, yielding a sample of 31 participants. MAIN MEASURES: Thematic analysis was carried out to analyse the interview data. RESULTS: Five themes were identified: beliefs, expectations, experiences of taking antibiotic, experience of antimicrobial resistance and side-effects, and experiences of consultations. The accounts reflected improved public knowledge: antibiotics were perceived to be much-needed medicines that should be prescribed when appropriate. The data showed that patients formed expectations of expectations, trying to read the prescribers' intentions and reflect on the dependency between what prescribers and patients wanted. Patient experiences featured as nuanced and detailed with knowledge of AMR and side-effects of antibiotics in the context of positive consultation experiences. CONCLUSIONS: The study highlighted complex interplays between adherence to antibiotics and consuming antibiotics in reflexive, informed ways. Ensuring that present and future patients are informed about potential benefits and harms of antibiotic use will contribute to future antimicrobial stewardship

Venture business and control of ь organizations under globalization

У статті розкрито діяльність венчурних компаній та особливості кожного етапу управління венчурним фінансуванням, яке в сучасних умовах є достатньо ризиковим. Проаналізовано стан венчурного підприємництва у світі та в Україні, окреслено головні проблеми, з якими зустрічаються вітчизняні венчурні підприємства та окреслено шляхи їх вирішення. Запропоновано спосіб формування системи венчурного контролінгу на промислових підприємствах, що полягає у виборі організаційного механізму управління службою контролінгу. Визначено етапи технології його проведення, пріоритетні напрями та елементи контролю на етапах життєвого циклу венчурного підприємства. У статті розкрито методичні положення та запропоновано практичні рекомендації з оцінювання та контролювання венчурної діяльності, а також запропоновано форму звітності за результатами венчурної діяльності. Вказано основні проблеми, на які повинні звернути увагу венчурні менеджери під час проходження кожного етапу венчурного проекту.В статье раскрыта деятельность венчурных компаний и особенности каждого этапа управления венчурным финансированием, которое в современных условиях является достаточно рискованным. Проанализировано состояние венчурного предпринимательства в мире и в Украине, определены главные проблемы, с которыми сталкиваются отечественные венчурные предприятия и намечены пути их решения. Предложен способ формирования системы венчурного контроллинга на промышленных предприятиях, заключается в выборе организационного механизма управления службой контроллинга. Обнародованы этапы технологии его проведения, приоритетные направления и элементы контроля на этапах жизненного цикла венчурного предприятия. В статье раскрыто методические положения и предложены практические рекомендации по оценке и контролю венчурной деятельности, а также предложено форму отчетности по результатам венчурной деятельности. Указано основные проблемы, на которые должны обратить внимание венчурные менеджеры при прохождении каждого этапа венчурного проекта.The activity of venture companies and peculiarities of each management stage of venture financing which in modern terms is quite risky has been described in the article. The state of venture entrepreneurship in the world and in Ukraine has been analyzed, the main problems faced by domestic venture enterprises have been outlined and ways of their solution have been determined. The method of forming the system for the venture controlling at industrial enterpriseswhich is to select organizational mechanism for management of controlling service has been proposed. It has been determined stages of technology for its conducting, priority directions and elements of control at the stages of the life cycle of venture enterprise. Methodological provisions have been revealed and practical recommendations on the evaluation and controlling the venture activity have been presented and the form of reporting by results of venture activity has been proposed in the article. It has been indicated the main problems that venture managers have to pay attention during the each stage of the venture project

Modern forms of foreign language distance learning in aquaculture

This article is devoted to a rather relevant topic of the 21st century. The subject of the analysis of the article is distance learning. The object of this article is a comparative analysis of distance learning in different countries. In recent years, the use of modern technical means has become a popular phenomenon in education all over the world. Information educational technologies have been intensively developed and increasingly used in the daily educational process along with traditional forms of education in many countries of the world. In this article, we consider the use of distance education in different countries, which helps to expand access to education and training for everyone. The choice of the topic is due to the relevance and the need to study distance education as an innovative form of education from various points of view. The authors analyze the use of distance learning sampled in various countries. The theoretical significance of the study lies in the fact that the data obtained can contribute to the deepened study of distance learning. The practical significance lies in the fact that the data obtained can be used in the course of modern pedagogy and methodology, in special courses on the problems of modern education

4-Thiazolidinone derivative Les-3833 effectively inhibits viability of human melanoma cells through activating apoptotic mechanisms

Aim To evaluate cytotoxic action of 4-thiazolidinone derivative Les-3833 and study the mechanisms of its pro-apoptotic action toward human melanoma cells and human tumor cell lines of other tissue origin. Methods The effect of Les-3833 or doxorubicin on the viability of 9 cell lines was studied using MTT assay, while human melanoma cells of WM793 line were additionally examined using light and fluorescent microscopies for evaluating cytomorphological changes. The Western-blot and flow cytometric analyses were carried out to study signaling pathways of melanoma cell cycling and death. Results Les-3833 was the most efficient against melanoma cells. Its half maximal inhibitory concentration (IC50) was 0.22 μg/mL for WM793 cells and 0.3 μg/mL for SK-Mel- 28 melanoma cells. For human lung A549, breast MCF-7, colon HCT116, and ovarian SKOV3 carcinoma cell lines IC50 was in between 2.5 to >5.0 μg/mL. Les-3833 was relatively not toxic (IC50 > 5 μg/mL) for human embryonic kidney HEK293 cells. Results of Annexin V/PI staining of melanoma cells and activation of caspase 3, PARP, MAPK, and EndoG protein suggest apoptosis in Les-3833-treated cells. Les- 3833 also induced ROS production in melanoma cells and their arrest in G0/G1 phase of cell cycle. Conclusion Novel 4-thiazolidinone derivative Les-3833 is effective against human melanoma cells in vitro, and such effect is tumor specific since it is much less pronounced in human carcinoma and leukemia cells. In melanoma cells Les-3833 induces apoptosis (morphological changes and increased pro-apoptotic proteins), ROS production, and arrest in G0/G1 phase of cell cycle

Risks of use and non-use of antibiotics in primary care: qualitative study of prescribers' views.

PURPOSE: The emergence of antimicrobial resistance has led to increasing efforts to reduce unnecessary use of antibiotics in primary care, but potential hazards from bacterial infection continue to cause concern. This study investigated how primary care prescribers perceive risk and safety concerns associated with reduced antibiotic prescribing. METHODS: Qualitative study using semistructured interviews conducted with primary care prescribers from 10 general practices in an urban area and a shire town in England. A thematic analysis was conducted. RESULTS: Thirty participants were recruited, including twenty-three general practitioners, five nurses and two pharmacists. Three main themes were identified: risk assessment, balancing treatment risks and negotiating decisions and risks. Respondents indicated that their decisions were grounded in clinical risk assessment, but this was informed by different approaches to antibiotic use, with most leaning towards reduced prescribing. Prescribers' perceptions of risk included the consequences of both inappropriate prescribing and inappropriate withholding of antibiotics. Sepsis was viewed as the most concerning potential outcome of non-prescribing, leading to possible patient harm and potential litigation. Risks of antibiotic prescribing included antibiotic resistant and Clostridium difficile infections, as well as side effects, such as rashes, that might lead to possible mislabelling as antibiotic allergy. Prescribers elicited patient preferences for use or avoidance of antibiotics to inform management strategies, which included educational advice, advice on self-management including warning signs, use of delayed prescriptions and safety netting. CONCLUSIONS: Attitudes towards antibiotic prescribing are evolving, with reduced antibiotic prescribing now being approached more systematically. The safety trade-offs associated with either use or non-use of antibiotics present difficulties especially when prescribing decisions are inconsistent with patients' expectations