Commonly used medications and endometrial cancer survival: a population-based cohort study.

Genomic Identification of Significant Targets (GISTIC) outputs for Circular Binary Segmentation (CBS) - or Piecewise Constant Fit (PCF) - segmented input data. The number of peaks attained by GISTIC on the y-axis is plotted against the two changing parameters α for CBS and γ for PCF on the x-axis. GISTIC peaks of amplification applying CBS-segmented data are illustrated in pink and PCF-segmented data in red, respectively. Deletion peaks are colored in green for CBS-segmented input data and in blue for PCF-segmented data. From top to bottom are shown GISTIC focal peaks for breast, ovarian, endometrial, and cervical cancers, to the left for PCF-segmented input data (A, C, E, and G) and to the right for CBS-segmented input data (B, D, F and H), respectively. For further analysis are the selected α and γ highlighted with a colored square. (PDF 362 kb

Penggunaan Media Gambar Dalam Meningkatkan Kemampuan Membaca Permulaan Siswa Kelas I SDN Uwedaka Kecamatan Pagimana Kabupaten Banggai

Pokok permasalahan dalam penelitian ini adalah rendahnya tingkat kemampuan membaca permulaan siswa kelas I SDN Uwedaka dalam pembelajaran Bahasa Indonesia. Tujuan Penelitian adalah untuk meningkatkan kemampuan membaca permulaan siswa kelas I SDN Uwedaka Kecamatan Pagimana Kabupaten Banggai. Berdasarkan hasil observasi yang didapatkan masih terdapat beberapa siswa yang sama sekali belum bisa membaca. Pembelajaran membaca permulaan di SDN Uwedaka selama ini hanya menggunakan media pembelajaran yang konvensional yaitu dengan menggunakan papan tulis, pembelajaran yang hanya berpusat pada guru, penggunaan media dalam pembelajaran sebagai alat bantu masih sangat terbatas, hal ini menyebabkan kemampuan membaca permulaan yang masih rendah dan terlihat hampir 65% siswa masih mengalami kesulitan membaca dalam proses belajar mengajar. Metode yang digunakan adalah metode deskriptif kualitatif dan kuantitatif. Data kualitatif didapatkan dari hasil tes dan observasi siswa dan guru. data kuantitatif didapatkan dari hasil tes belajar. Desain penelitian ini mengacu pada desain oleh Kemmis dan Mc Taggart yang terdiri dari empat tahapan, yaitu perencanaan, pelaksanaan tindakan, observasi dan refleksi. Data dikumpulkan melalui penilaian proses dan penilaian hasil setiap akhir tindakan. Penelitian ini dilakukan dalam dua siklus. Pada siklus I diperoleh nilai rata-rata siswa yaitu sebesar 67 dengan ketuntasan belajar klasikal sebesar 40% serta daya serap 66,6%. Pada siklus II, nilai rata-rata meningkat menjadi 83 dengan ketuntasan klasikal sebesar 100% serta daya serap klasikal sebesar 83,3%. Bersarkan hasil penelitian maka dapat disimpulkan bahwa penggunaan media gambar dapat meningkatkan kemampuan membaca permulaan terhadap siswa kelas I SDN Uwedaka Kecamatan Pagimana Kabupaten Banggai

Additional file 7: Table S5. of A systematic comparison of copy number alterations in four types of female cancer

GISTIC focal peaks for selected α (CBS) and γ (PCF) - gains and losses. Table S5 reveals the GISTIC focal peaks of the selected values in Table S4. Indicated by asterisks (*) are the joint regions between cancer types for at least two cancers displaying a common genomic region, and (n) representing the number of events in each cohort. (XLSX 37 kb

Additional file 3: Table S2. of A systematic comparison of copy number alterations in four types of female cancer

GISTIC focal peaks for simulated data – gains and losses. Table S2A exemplifies the number of focal peaks of simulation data for PCF-segmented input data to GISTIC and variable γ from 10 to 90. Table S2B represents GISTIC focal peaks of simulated data for CBS-segmented input data to GISTIC and α varied from 0.00001 to 0.1. (XLSX 10 kb

Additional file 4: of Artesunate shows potent anti-tumor activity in B-cell lymphoma

Figure S3. The tumor load is evenly distributed among the treatment and control groups. (A) Tumor growth was monitored with IVIS luminescence measurements (physical units of surface radiance, photons/s/cm2/sr). Image was taken at day 0 when treatment was started (day 4 after injection of BL-41-luc). (B) Graphical distribution of luminescence measurements at treatment start. (C) Artesunate treatment does not affect the weight of the mice. An increased weight was observed for both groups during the treatment. Shown is weight (g) after treatment start (n = 10 for each group). Weight at start was 16.0-22.8 g and the mice were 6-10 weeks old. (EPS 3428 kb

Additional file 1: of Artesunate shows potent anti-tumor activity in B-cell lymphoma

Table S1. Overview of the additional drugs used in the first line screen. (DOCX 36 kb

Additional file 5: of Artesunate shows potent anti-tumor activity in B-cell lymphoma

Table S2. The top five pathways from the ingenuity pathway analysis (IPA) show UPR as the top regulated pathway in all three cell lines with corresponding p-value and percentage overlap of genes. (DOCX 232 kb

Additional file 7: of Artesunate shows potent anti-tumor activity in B-cell lymphoma

Figure S5. IPA upstream regulators analysis predicts MYC to be inhibited. (A) In the top list of the upstream regulators analyzed by IPA, ATF4 and TRIB3 are among the top genes. MYC on the other hand is listed as number ten. The activation z-score predicts if a gene is an upstream activator (red) or an upstream inhibitor (blue). This means that the genes are predicted to regulate other genes, which are shown in B for MYC. (B) Figure shows upstream regulated network for MYC in BL-41, Oci-Ly-2 and SU-DHL-4 merged and show an interaction map between MYC and other regulated genes in the arrays. The dark blue color of MYC means IPA predicts inhibiton of MYC with “more confidence”. The goal of the Regulator Effects analysis in IPA is to provide insight into the causes and effects of differentially expressed genes or proteins in a dataset, and how they might cause differences in downstream outcomes. (EPS 1096 kb

Additional file 2: of Artesunate shows potent anti-tumor activity in B-cell lymphoma

Figure S1. Artesunate induces apoptosis in B-cell lymphoma cell lines. (A) Dose response curves for the four most responsive cell lines and healthy CD19+ B-cells from Fig. 2a. Relative viability indicates relative luminescence units [RLU] from the CellTiter glo assay (n = 3-5, 0.125-8 μM artesunate, 72 h). (B) Propidium iodide (PI) staining detects a high level of dead cells in artesunate-exposed B-cell lymphoma cell lines (5 μM, P < .046, 10 μM, P < 0.05) compared to healthy B-cells (not significant (n.s)). Cells were treated with artesunate (5 and 10 μM, 72 h, n = 3) and stained with PI. The side scatter (SSC)- PI plot shows gating of the PI positive cells in untreated and artesunate treated BL-41 cells. (C) Artesunate induces apoptosis in B-cell lymphoma cell lines (P < 0.04), but not in healthy B-cells (n.s). Staining for active caspase 3 in lymphoma cell lines was done after artesunate treatment (5 μM art, 24 h, n = 3), fixation and permeabilization (PFA/methanol). The plots show example of gating for the positive active caspase 3 populations in both untreated and artesunate treated BL-41 cells. The cells are first gated on single cells, then living cells in forward/side scatter. (EPS 1906 kb

Additional file 8: of Artesunate shows potent anti-tumor activity in B-cell lymphoma

Figure S6. BSO treatment decreases the levels of GSH and attenuates artesunate in lymphoma cell lines. (A) GSH-Glo glutathione assay was used to measure GSH levels after treatment with BSO (50 μM, 24 h) in BL-41, SU-DHL-6 and normal CD19+ B-cells with or without IL21. (B) BSO does not decrease cell viability in lymphoma cell lines after 24 h treatment (50 μM), a small decrease in cell viability was seen in normal B-cells (n.s). (C) Cells were treated with BSO (50 μM) for 24 h before co-treatment with increasing concentration of artesunate for 24 h. Pre-treatment with BSO induced an increased sensitivity to artesunate in malignant cells, but not in normal B-cells (n = 3 (cell lines), n = 2 CD19+ cells; two different donors). (EPS 1102 kb