1 research outputs found
Decreased rotavirus infection of MA104 cells via probiotic extract binding to Hsc70 and 脽3 integrin receptors
Probiotic bacteria are microorganisms beneficial to human health, useful to improving biological conditions. Thanks to probiotic bacteria the symptoms of viral infections can be alleviated. Different mechanisms whereby probiotic bacteria exert they antiviral effect have been proposed. The aim of this study was to determine whether probiotic bacteria extracts bind to receptors of host cells susceptible of rotavirus (RV) infection. To accomplish this objective, four probiotic bacterial strains of Lactobacillus spp. and Bifidobacterium spp. were tested. Probiotic extracts were obtained after bacterial growth, cell lysis and centrifugation. Obtained probiotic extracts were used in assays to interfere with adhesion and penetration of a RV strain in the mammal cell line MA104. Furthermore, the interaction between probiotic extracts and MA104 cell receptors was evaluated by co-immunoprecipitation assays using anti-脽3-integrins and anti-Hsc70 antibodies. All four probiotic, protein-rich, extracts reduced RV infections in MA104 cells, suggesting a successful antiviral activity mediated by these probiotic extracts. All probiotic extracts significantly exerted thir antiviral activity by interfering with RV adhesion on MA104 cell receptors, with proteins in probiotic extracts competitively interacting with cell surface receptors necessary to RV infection. Co-immunoprecipitation assay results showed that proteins in probiotic extracts were able to bind to 脽3-integrinsand Hsc70, which are two cellular receptors required to viral infection. The most significant contribution of this study is an insight into the mechanisms of probiotic antiviral activity, thus expanding current probiotics fundamental knowledge.Las bacterias probi贸ticas son microorganismos con efectos positivos en la salud humana, gracias a las bacterias probi贸ticas los s铆ntomas de infecciones virales pueden mitigarse. Al respecto, varios mecanismos antivirales de las bacterias probi贸ticas han sido propuestos. El prop贸sito de este estudio fue determinar, de manera experimental, si extractos de bacterias probi贸ticas reducen la infecci贸n rotav铆rica al interferir con la uni贸n entre el rotavirus y sus receptores celulares blanco. Extractos de cuatro cepas probi贸ticas de Lactobacillus spp. y Bifidobacterium spp. fueron obtenidos a partir de cultivos bacterianos lisados y centrifugados. Cada uno de los extractos fue usado en experimentos para determinar si estos interfieren con la adhesi贸n y penetraci贸n del rotavirus en c茅lulas de mam铆fero MA104. Adem谩s, la interacci贸n entre extractos probi贸ticos y receptores de las c茅lulas MA104 fue evaluada con ensayos de co-inmunoprecipitaci贸n, usando anticuerpos anti-integrina 尾3 y anti-Hsc70. Se observ贸 que los cuatro extractos probi贸ticos, ricos en prote铆nas, redujeron significativamente la infecci贸n de rotavirus en las c茅lulas MA104. Tambi茅n se estableci贸 que la que la actividad antiviral de los extractos probi贸ticos es mediada por la interacci贸n competitiva de sus prote铆nas con los receptores integrina 尾3 y Hsc70 de las c茅lulas MA104, necesarios para iniciar la infecci贸n por rotavirus. Estos hallazgos constituyen un aporte al conocimiento de los mecanismos b谩sicos de acci贸n antiviral de las bacterias probi贸ticas