352 research outputs found
Regeneration and genetic transformation in cowpea
Over the last three decades, sporadic efforts have been made to develop regeneration
and transformation systems in cowpea (Vigna unguiculata L. Walp). This paper
reviews the progress made to date, including highlights of culture media and
explants used for regeneration and chimeric gene constructs employed in transformations.
Progress has been slow, mainly due to limited resources, since very few
laboratories have been involved. There is an urgent need for more focused and
consistent efforts to develop genotype, and tissue-culture dependent and independent
approaches for obtaining stable genetic transformation in cowpea
Use of somatic embryogenesis as a vehicle for cotton transformation
Cotton has been aptly described as the prosperity plant owing to its unrivalled economic importance as
a source of feedstock, food and oil, as well as raw material for diverse industrial applications, ranging
from textile and footwear to automobiles, energy, medical and pharmaceutical. As such, over 180
million people of the world depend on its production for livelihood. However, cotton production is
grossly hampered, and has long been peaked in many regions where it is being grown. Without
prejudice to the genetic improvement already made by conventional breeding with respect to yield and
quality over the years, genetic transformation is arguably the last recourse for further development of
cotton, especially with respect to the prevailing production constraints of insect pests, weeds,
environmental stresses and diseases. This review therefore focuses on the use of somatic
embryogenesis as a vehicle for cotton genetic transformation. It indeed attempts to overview the
challenges of cotton transformation with respect to narrow genetic base coupled with the recalcitrant
nature of the crop species, as well as the research success achieved so far. It then discusses the
underlying mechanisms of somatic embryogenesis as well as the current constraints and various
strategies being used to overcome them; all with the aim of motivating interest groups to initiate
research activities in cotton biotechnology and to strive for its optimization for further genetic
improvement
Irregular deposition of cell wall polymers resulting from defective cellulose synthase complexes
The crystalline cellulose microfibril is formed by
the spontaneous association of about 36 β-D-glucan
chains, which are simultaneously synthesised by a large
membrane-localised multi-enzyme cellulose synthase
complex. Antisense technology has been previously
employed separately on two of the constituent cellulose
synthase catalytic subunits (CesA) of the complex in
potato (Solanum tuberosum), namely CesA2 and CesA4,
to generate potato tuber cell walls with reduced cellulose
content. Genetic crossing of two transgenic potato lines
csr2-1 and csr4-8 was carried out to investigate the
effects of two defective CesAs in the same genetic
background, with respect to cellulose deposition in the
potato tuber cell walls. It was striking to observe, through
fluorescence microscopy with calcofluor white, a strong
fluorescence in the cell corners and less prominent and
uneven fluorescence around the cells of the csr2 tubers
as compared to others. It was also noted that these
phenotypes were not pronounced in the csr2/csr4 double
transformants as expected
Endogenous levels of reducing sugars, free amino acids and phenols during various stages of in vitro culture of cotton (Gossypium Spp.)
Somatic embryogenesis is widely preferred as the regeneration route for in vitro studies in cotton.
However, the regeneration efficiency through this approach is low; a problem that is believed to be as a result
of the biochemical properties of the plant. The objective of this study was to investigate possible relationships
between three biochemical factors (reducing sugars, phenols, and free amino acids) and somatic
embryogenesis. In vitro cultures of the different embryogenic and non-embryogenic cultivals were established.
The levels of reducing sugars, phenols and free amino acids were determined at different developmental stages
of the cultures. Higher levels of reducing sugars and lower level of phenol were observed in embryogenic
cultivars compared to their non-embryogenic counterparts. There was a general increase in the levels of free
amino acids, which decreased with time in the highly embryogenic cultivars, whereas the levels remained high
in the poorly embryogenic and non-embryogenic cultivars. The higher content of phenols and free amino acids
may be implicated in the poor somatic embryogenic response. The data show that there are factors that may
serve as markers of somatic embryogenesis in cotton, which need to be empirically determined for any
particular cultivar chosen for genetic improvement through embryogenesis
The plant biotechnology flight: Is Africa on board?
The development of plant biotechnologies has been very rapid in recent times, especially in the
developed countries. The technologies have created a new branch of biotechnology known as
molecular farming, where plants are engineered to produce pharmaceutical and technical proteins in
large quantities. An evaluation of the status of plant biotechnology development in Africa revealed that
majority of the countries that are involved in biotech activities are still at the level of tissue culture
applications. This calls for urgent and sincere commitments on the part of the various stakeholders in
Africa, especially the governments, to the development of biotechnology capacity
Expression of an expansin carbohydrate-binding module affects xylem and phloem formation
Expansins are believed to be involved in disrupting the non-covalent adhesion of cellulose to matrix
polysaccharides, thereby promoting wall creep. We have targeted a putative potato expansin (EXPA)
carbohydrate-binding module (CBM) to the cell walls of tobacco plants. Histological examinations and
electron microscopy indicated that 30% of the xylem cells of the transgenic stems with high expression
of the expansin CBM are wider (radial surface area) than those of the controls. Similarly, 37% of the
xylem cells of the stems of the high expressers have thinner cell walls than those of the controls. There
were no such phenotypes in the low and none expressers, as well as in the control plants. The
transgenic tobacco plants expressing the potato expansin CBM did not exhibit marked change in plant
morphology. Analysis of cellulose content in the stem cell walls was similar between the high expresser
of the transgene and the control plants. Nonetheless, our results taken together demonstrate that
expansin CBM alone can bring about changes in the plant cell walls
Expression of the C-terminal family 22 carbohydratebinding module of xylanase 10B of Clostridium themocellum in tobacco plant
Carbohydrate-binding modules have been shown to alter plant cell wall structural architecture. Hence,
they have the potential application of being used to engineer the plant to produce tailor-made natural
fibers in the cell wall. The Clostridium thermocellum xylanase, Xyn10B, contains two CBMs that belong
to family 22 (CBM22). The C-terminal CBM22-2 of the glycoside hydrolase (GH) 10 had been
characterized to interact with xylan, a major hemicellulosic component in the secondary cell wall of
plants. In this work, the expression of the CBM22-2 in transgenic tobacco plants was evaluated.
Histological examinations of the transgenic stems did not reveal marked cell wall phenotype. In
addition, there were no observable changes in the height or the appearance of the transgenic plants
expressing the CBM22-2 module. The results indicate that the family 22 carbohydrate binding module is
not a potential candidate for use in in planta modification of the cell wall
STUDIES ON KOLA TISSUE CULTURE I: Protocols for Establishing Kola Tissues in vitro
The micropropagation of Cola nitida (Vent.) Schott and Endlicher by means of tissue culture was
investigated to provide baseline infonnation on the requ irements for the survival of kola tissues and
organs in vitro. Investigations were conducted on development of sterilization protocols, and medium
selection and modification. The best sterilization procedure was established with the step-wise
treatment of 70% ethanol, for 20 seconds and 10% (w/v) CaOCl, for I 0 minutes .. The use of modified
Murashige-Skoog (MS) medium (without Zn and Cu elements) as basal medium was found as
appropriate as the original MS medium, for explants' survival. The appropriate antioxidant technique
was also established with 10mgl-1 ascorbic acid
Progeny from the crosses of two antisense potato plants exhibit ectopic xylem differentiation
Progeny from the crosses of two transgenic potato lines csr2-1 and csr4-8, containing two different
antisense constructs, csr2 and csr4 had been previously characterized to exhibit altered tuber
production. Histochemical staining and microscopic examinations of the tubers were made to
investigate cellular phenotype in the tubers. We observed ectopic proliferation of xylem, which is most
pronounced in the csr2 tubers. Light microscopy of csr2 tubers revealed that the proliferation of xylem
was associated with lignification of their cell walls. This paper provides evidence of cellular phenotype
as a consequence of the presence of the antisense construct
Seed pretreatments enhance germination in Occimum gratissimum (lameaceae)
Occimum gratissimum (fever plant) is a crop with a wide range of use as a medicinal plant and as a spice. In spite of
its huge economic importance, the plant is only grown in homestead. The study investigated the effects of light, temperature
and water treatments on the germination of the seeds. The incubation of seeds at 25 °C under light condition,
coupled with leaching in running water for 12 hours was found to release the dormancy in the seeds of Occimum gratissimum
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