132 research outputs found
Propagating front in an excited granular layer
A partial monolayer of ~ 20000 uniform spherical steel beads, vibrated
vertically on a flat plate, shows remarkable ordering transitions and
cooperative behavior just below 1g maximum acceleration. We study the stability
of a quiescent disordered or ``amorphous'' state formed when the acceleration
is switched off in the excited ``gaseous'' state. The transition from the
amorphous state back to the gaseous state upon increasing the plate's
acceleration is generally subcritical: An external perturbation applied to one
bead initiates a propagating front that produces a rapid transition. We measure
the front velocity as a function of the applied acceleration. This phenomenon
is explained by a model based on a single vibrated particle with multiple
attractors that is perturbed by collisions. A simulation shows that a
sufficiently high rate of interparticle collisions can prevent trapping in the
attractor corresponding to the nonmoving ground state.Comment: 16 pages, 9 figures, revised version, to appear in Phys. Rev. E, May
199
Dynamics of electrostatically-driven granular media. Effects of Humidity
We performed experimental studies of the effect of humidity on the dynamics
of electrostatically-driven granular materials. Both conducting and dielectric
particles undergo a phase transition from an immobile state (granular solid) to
a fluidized state (granular gas) with increasing applied field. Spontaneous
precipitation of solid clusters from the gas phase occurs as the external
driving is decreased. The clustering dynamics in conducting particles is
primarily controlled by screening of the electric field but is aided by
cohesion due to humidity. It is shown that humidity effects dominate the
clustering process with dielectric particles.Comment: 4 pages, 4 fig
Non-Gaussian Velocity Distribution Function in a Vibrating Granular Bed
The simulation of granular particles in a quasi two-dimensional container
under the vertical vibration as an experimental accessible model for granular
gases is performed. The velocity distribution function obeys an
exponential-like function during the vibration and deviates from the
exponential function in free-cooling states. It is confirmed that this
exponential-like distribution function is produced by Coulomb's friction force.
A Langevin equation with Coulomb's friction is proposed to describe the motion
of such the system.Comment: 4 pages, 4 figures. to be published in Journal of Physical Society of
Japan Vol.73 No.
An affinity matured minibody for PET imaging of prostate stem cell antigen (PSCA)-expressing tumors
PurposeProstate stem cell antigen (PSCA), a cell surface glycoprotein expressed in normal human prostate and bladder, is over-expressed in the majority of localized prostate cancer and most bone metastases. We have previously shown that the hu1G8 minibody, a humanized anti-PSCA antibody fragment (single-chain Fv-C(H)3 dimer, 80 kDa), can localize specifically and image PSCA-expressing xenografts at 21 h post-injection. However, the humanization and antibody fragment reformatting decreased its apparent affinity. Here, we sought to evaluate PET imaging contrast with affinity matured minibodies.MethodsYeast scFv display, involving four rounds of selection, was used to generate the three affinity matured antibody fragments (A2, A11, and C5) that were reformatted into minibodies. These three affinity matured anti-PSCA minibodies were characterized in vitro, and following radiolabeling with (124)I were evaluated in vivo for microPET imaging of PSCA-expressing tumors.ResultsThe A2, A11, and C5 minibody variants all demonstrated improved affinity compared to the parental (P) minibody and were ranked as follows: A2 > A11 > C5 > P. The (124)I-labeled A11 minibody demonstrated higher immunoreactivity than the parental minibody and also achieved the best microPET imaging contrast in two xenograft models, LAPC-9 (prostate cancer) and Capan-1 (pancreatic cancer), when evaluated in vivo.ConclusionOf the affinity variant minibodies tested, the A11 minibody that ranked second in affinity was selected as the best immunoPET tracer to image PSCA-expressing xenografts. This candidate is currently under development for evaluation in a pilot clinical imaging study
Scaling, Multiscaling, and Nontrivial Exponents in Inelastic Collision Processes
We investigate velocity statistics of homogeneous inelastic gases using the
Boltzmann equation. Employing an approximate uniform collision rate, we obtain
analytic results valid in arbitrary dimension. In the freely evolving case, the
velocity distribution is characterized by an algebraic large velocity tail,
P(v,t) ~ v^{-sigma}. The exponent sigma(d,epsilon), a nontrivial root of an
integral equation, varies continuously with the spatial dimension, d, and the
dissipation coefficient, epsilon. Although the velocity distribution follows a
scaling form, its moments exhibit multiscaling asymptotic behavior.
Furthermore, the velocity autocorrelation function decays algebraically with
time, A(t)= ~ t^{-alpha}, with a non-universal dissipation-dependent
exponent alpha=1/epsilon. In the forced case, the steady state Fourier
transform is obtained via a cumulant expansion. Even in this case, velocity
correlations develop and the velocity distribution is non-Maxwellian.Comment: 10 pages, 3 figure
NMR Experiments on a Three-Dimensional Vibrofluidized Granular Medium
A three-dimensional granular system fluidized by vertical container
vibrations was studied using pulsed field gradient (PFG) NMR coupled with
one-dimensional magnetic resonance imaging (MRI). The system consisted of
mustard seeds vibrated vertically at 50 Hz, and the number of layers N_ell <= 4
was sufficiently low to achieve a nearly time-independent granular fluid. Using
NMR, the vertical profiles of density and granular temperature were directly
measured, along with the distributions of vertical and horizontal grain
velocities. The velocity distributions showed modest deviations from
Maxwell-Boltzmann statistics, except for the vertical velocity distribution
near the sample bottom which was highly skewed and non-Gaussian. Data taken for
three values of N_ell and two dimensionless accelerations Gamma=15,18 were fit
to a hydrodynamic theory, which successfully models the density and temperature
profiles including a temperature inversion near the free upper surface.Comment: 14 pages, 15 figure
The Physics of the Colloidal Glass Transition
As one increases the concentration of a colloidal suspension, the system
exhibits a dramatic increase in viscosity. Structurally, the system resembles a
liquid, yet motions within the suspension are slow enough that it can be
considered essentially frozen. This kinetic arrest is the colloidal glass
transition. For several decades, colloids have served as a valuable model
system for understanding the glass transition in molecular systems. The spatial
and temporal scales involved allow these systems to be studied by a wide
variety of experimental techniques. The focus of this review is the current
state of understanding of the colloidal glass transition. A brief introduction
is given to important experimental techniques used to study the glass
transition in colloids. We describe features of colloidal systems near and in
glassy states, including tremendous increases in viscosity and relaxation
times, dynamical heterogeneity, and ageing, among others. We also compare and
contrast the glass transition in colloids to that in molecular liquids. Other
glassy systems are briefly discussed, as well as recently developed synthesis
techniques that will keep these systems rich with interesting physics for years
to come.Comment: 56 pages, 18 figures, Revie
Transport Coefficients for Granular Media from Molecular Dynamics Simulations
Under many conditions, macroscopic grains flow like a fluid; kinetic theory
pred icts continuum equations of motion for this granular fluid. In order to
test the theory, we perform event driven molecular simulations of a
two-dimensional gas of inelastic hard disks, driven by contact with a heat
bath. Even for strong dissipation, high densities, and small numbers of
particles, we find that continuum theory describes the system well. With a bath
that heats the gas homogeneously, strong velocity correlations produce a
slightly smaller energy loss due to inelastic collisions than that predicted by
kinetic theory. With an inhomogeneous heat bath, thermal or velocity gradients
are induced. Determination of the resulting fluxes allows calculation of the
thermal conductivity and shear viscosity, which are compared to the predictions
of granular kinetic theory, and which can be used in continuum modeling of
granular flows. The shear viscosity is close to the prediction of kinetic
theory, while the thermal conductivity can be overestimated by a factor of 2;
in each case, transport is lowered with increasing inelasticity.Comment: 14 pages, 17 figures, 39 references, submitted to PRE feb 199
Growth delay of human bladder cancer cells by Prostate Stem Cell Antigen downregulation is associated with activation of immune signaling pathways
<p>Abstract</p> <p>Background</p> <p>Prostate stem cell antigen (PSCA) is a glycosylphosphatidylinositol (GPI) anchored protein expressed not only in prostate but also in pancreas and bladder cancer as shown by immunohistochemistry and mRNA analysis. It has been targeted by monoclonal antibodies in preclinical animal models and more recently in a clinical trial in prostate cancer patients. The biological role played in tumor growth is presently unknown. In this report we have characterized the contribution of PSCA expression to tumor growth.</p> <p>Methods</p> <p>A bladder cell line was engineered to express a doxycycline (dox) regulated shRNA against PSCA. To shed light on the PSCA biological role in tumor growth, microarray analysis was carried out as a function of PSCA expression. Expression of gene set of interest was further analyzed by qPCR</p> <p>Results</p> <p>Down regulation of the PSCA expression was associated with reduced cell proliferation <it>in vitro </it>and <it>in vivo</it>. Mice bearing subcutaneous tumors showed a reduced tumor growth upon treatment with dox, which effectively induced shRNA against PSCA as revealed by GFP expression. Pathway analysis of deregulated genes suggests a statistical significant association between PSCA downregulation and activation of genes downstream of the IFNα/β receptor.</p> <p>Conclusions</p> <p>These experiments established for the first time a correlation between the level of PSCA expression and tumor growth and suggest a role of PSCA in counteracting the natural immune response.</p
Hemolymph microbiome of Pacific oysters in response to temperature, temperature stress and infection
Microbiota provide their hosts with a range of beneficial services, including defense from external pathogens. However, host-associated microbial communities themselves can act as a source of opportunistic pathogens depending on the environment. Marine poikilotherms and their microbiota are strongly influenced by temperature, but experimental studies exploring how temperature affects the interactions between both parties are rare. To assess the effects of temperature, temperature stress and infection on diversity, composition and dynamics of the hemolymph microbiota of Pacific oysters (Crassostrea gigas), we conducted an experiment in a fully-crossed, three-factorial design, in which the temperature acclimated oysters (8 or 22 °C) were exposed to temperature stress and to experimental challenge with a virulent Vibrio sp. Strain. We monitored oyster survival and repeatedly collected hemolymph of dead and alive animals to determine the microbiome composition by 16s rRNA gene amplicon pyrosequencing. We found that the microbial dynamics and composition of communities in healthy animals (including infection survivors) were significantly affected by temperature and temperature stress, but not by infection. The response was mediated by changes in the incidence and abundance of operational taxonomic units (OTUs) and accompanied by little change at higher taxonomic levels, indicating dynamic stability of the hemolymph microbiome. Dead and moribund oysters, on the contrary, displayed signs of community structure disruption, characterized by very low diversity and proliferation of few OTUs. We can therefore link short-term responses of host-associated microbial communities to abiotic and biotic factors and assess the potential feedback between microbiota dynamics and host survival during disease
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