Knowledge, Attitude and Practice of Blood Conservation Strategies amongst Physicians’ in Tertiary Hospitals

Aim: To assess the extent of available knowledge, as well as the attitudes and the utilization of blood conservation strategies amongst medical doctors, who are the implementers of blood transfusion and conservation in clinical practice. Study Design: A cross-sectional study. Place and Duration of Study: Clinical Departments in both Lagos State University Teaching Hospital, Lagos State and Babcock University Teaching Hospital, Ogun State between August 2013 and November 2013. Methodology: We included clinicians from various medical specialties in the study (78 from public hospital and 26 from private hospital). A pre-tested, self-administered questionnaire was devised to collect data. Data was entered and analyzed descriptively and qualitatively. Result: A total of 104 doctors participated in the study consisting of 57 males (54.80%) and 47 females (45.29%). Almost all participants had transfused blood in their practice and all participants were aware that blood transfusions have complications. Majority 80 (76.9%) had heard about blood conservation techniques, about 51 (49%) had used any of the strategies. If available, 93 (89.4%) would use any of the strategies, only 1 (1%) would not. Conclusion: There is a need for hospital transfusion committees to educate and increase the drive for utilization of blood conservation methods amongst doctors, so as to increase its practice

Operations Research, Mathematics, Computer Science and Statistics: The Relationships

Many people have difficulty in seeing any difference between Mathematics, Operations Research, Statistics, Computer Science and other disciplines while others are just plain confused. In this work, OR and its applications are being exposed and then compared in order to look into the relationships between OR, Mathematics, Computer Science, Statistics and other fields. It has been realized that all these areas of knowledge are also interrelated with other areas such as Engineering, Physics, Microbiology, Economics etc

Ebolavirus is evolving but not changing: No evidence for functional change in EBOV from 1976 to the 2014 outbreak

The 2014 epidemic of Ebola virus disease (EVD) has had a devastating impact in West Africa. Sequencing of ebolavirus (EBOV) from infected individuals has revealed extensive genetic variation, leading to speculation that the virus may be adapting to humans, accounting for the scale of the 2014 outbreak. We computationally analyze the variation associated with all EVD outbreaks, and find none of the amino acid replacements lead to identifiable functional changes. These changes have minimal effect on protein structure, being neither stabilizing nor destabilizing, are not found in regions of the proteins associated with known functions and tend to cluster in poorly constrained regions of proteins, specifically intrinsically disordered regions. We find no evidence that the difference between the current and previous outbreaks is due to evolutionary changes associated with transmission to humans. Instead, epidemiological factors are likely to be responsible for the unprecedented spread of EVD

Adaptive HIV-1 evolutionary trajectories are constrained by protein stability

Despite the use of combination antiretroviral drugs for the treatment of HIV-1 infection, the emergence of drug resistance remains a problem. Resistance may be conferred either by a single mutation or a concerted set of mutations. The involvement of multiple mutations can arise due to interactions between sites in the amino acid sequence as a consequence of the need to maintain protein structure. To better understand the nature of such epistatic interactions, we reconstructed the ancestral sequences of HIV-1's Pol protein, and traced the evolutionary trajectories leading to mutations associated with drug resistance. Using contemporary and ancestral sequences we modelled the effects of mutations (i.e. amino acid replacements) on protein structure to understand the functional effects of residue changes. Although the majority of resistance-associated sequences tend to destabilise the protein structure, we find there is a general tendency for protein stability to decrease across HIV-1's evolutionary history. That a similar pattern is observed in the non-drug resistance lineages indicates that non-resistant mutations, for example, associated with escape from the immune response, also impacts on protein stability. Maintenance of optimal protein structure therefore represents a major constraining factor to the evolution of HIV-1

Response of okra (Abelmoschus esculentus (L.) Moench) to weed control by mulching

The effects of mulching material on okra performance during the rainy seasons of 1999 and 2000 were evaluated at the Teaching and Research Farm of the Ladoke Akintola University of Technology, Ogbomoso, Nigeria. Three mulch types–plastic mulch, grass mulch (Panicum maximum) and wood shavings (of Tectonia grandis)– were compared with hand weeding and no weeding control in a randomized complete block experiment with three replications. Growth and yield characteristics of okra were assessed together with weed control efficiency under the five treatments. Okra growth and yield were significantly (P hoe weeding > wood shavings > grass mulch.Les expériences se sont déroulées au champ d'enseignement et de recherche de l'Université de Technology de Ladoke Akintola à Ogbomoso pour évaluer les effets de matériel de paillis sur le rendement de gombo pendant les saisons des pluies de 1999 et 2000. Trois types de paillis-paillis en plastique, paillis en herbe (Panicum maximum) et paillis en copeaux de bois (de Tectonia grandis) étaient comparés avec le désherbage à la main et le contrôle de non désherbage dans une expérience de bloc complet choisi au hasard avec trois reproductions. Les paramètres de croissance et de rendement de gombo étaient évalués ensemble avec l'efficacité du contrôle de mauvaise herbe sous les cinq traitements. La croissance et le rendement de gombo étaient considérablement (P sarclage > copeaux de bois > paillis en herbe. Ghana Journal of Agricultural Science Vol. 39 (1) 2006: pp. 35-4

Influence of Weed Control Methods on Viability and Vigour of Maize (Zea mays L.) Seeds

Weed control method of choice should achieve the objective of controlling weeds without sacrificing seed quality. The influence of Combination of mechanical method (slashing at 2, and 4 week intervals), and herbicide (pre-emergence and post emergence) for weed control in maize on seed quality (viability and vigour) of maize seeds were evaluated.. Field and laboratory experiments were conducted to investigate the influence of pre-emergence (Diuron 50% SC {N (3, 4-dichlorophenyl) N, N-dimethyl urea} and post-emergence (Atrazine 50FW-{6 chloro-N-methyl-N-(1-methylethyl) 1, 3, 5-triazine, 2, 4 diamine}) herbicides either alone, or in combination with slashing at two and four-week, on seed quality. Combination of pre-emergence herbicide and slashing 2-weeks after planting  slashing gave the highest viability (91.37%) and lowest conductivity value (2.79 µS/cm/g), whereas combination of post emergence herbicide and 4 week slashing gave the lowest viability (77.67%) and the highest conductivity value (15.71 µS/cm/g). In accelerated ageing test, combination of pre-emergence herbicide with either 2 week slashing, or 4 week slashing  WAP gave the highest viability (84%), whereas combination of post emergence herbicide with 2 week slashing gave the lowest viability (51.33%) and lowest germination rate index (10.37). Combination of pre-emergence herbicide and 2-week slashing is the best complementary option for weed control in maize seed crop. Keywords; pre-emergence, post-emergence herbicides,  seed physiology,  maize seed viabilit

CORPORATE GOVERNANCE AND HUMAN RESOURCE MANAGEMENT IN NIGERIA’S DOWNSTREAM PETROLEUM SECTOR

The emergence of a ‘new world economy’ makes it imperative for corporate entities to adjust their corporate values, practices and internal processes. This paper explored the interrelatedness of selected corporate governance practices and human resource management outcomes. The paper relied on established corporate management theories as a platform for empirical consideration of selected issues relative to four established players in Nigeria’s downstream petroleum sector. A descriptive method was adopted and data was collected via a survey of 112 respondents. Contextual arguments were captured to achieve a robust appreciation of issues affecting individual participation and operations of corporate entities. The study found that there is a significant relationship between corporate governance practices and human resource management outcomes. Requisite conclusions and recommendations were provided in the light of empirical and theoretical findings

Prevalence of pulmonary cryptococcosis in hiv/aids patients

Cryptococcus neoformans causes both pulmonary and meninges infection in healthy and immunocompromised hosts. The objective of this study was to determine the prevalence of pulmonary cryptococcosis in HIV/AIDS patients and assess the clinical presentations due to the infection. The subjects recruited for this study were confirmed HIV/AIDS patients, presenting with pneumonia complications. The demographic and clinical features were abstracted from the medical records and administered questionnaire. Early morning sputum samples were collected from the patients and examined by bacteriological methods. The isolated Cryptococcus neoformans were sub-cultured onto thistle bird seed agar and the identity was confirmed with API 20C Kit. Out of 250 patients investigated, 47 (18.8%) had pulmonary cryptococcosis. The C.neoformans isolates were more in females, 26 (10.4%) than the males, 21 (8.4%), while the age group of 20-40 years old had 39 (15.6%) isolates. The clinical features that were associated with the pulmonary cryptococcosis were the productionof purulent and bloody sputum, and fever. The patients with prolonged duration of HIV/AIDS and are not on HAART regimens had higher C.neoformans isolates. The isolation of C.neoformans from HIV/AIDS patients is a major public health concern, since it complicatesthe severity of the disease. There is a need for routine check of pulmonary cryptococcosis, especially, in those who do not avail themselves for antiretroviral therapy.Keywords: Cryptococcus neoformans, Cryptoccosis, HIV/AIDS, HAART, Thistle bird seed agar

Prevalence of Rhesus C and D Alloantibodies among Rhesus-Negative Women of Child Bearing Age at a Tertiary Hospital in South-West Nigeria

Background: A major cause of hemolytic disease of the fetus and newborn (HDFN) is an incompatibility of the Rhesus (Rh) blood group between the mother and fetus. Aim: To determine the prevalence of Rh c and D alloantibodies among Rh-negative women of childbearing age (18–49 years). We conducted a cross-sectional study among women who attended the antenatal, gynecology and blood donor clinics at a Tertiary Hospital in South-West Nigeria from January to August 2019. Serological typing of Rh c and D was done manually with the tube test using anti-c and anti-D antisera, while indirect antiglobulin test was then performed to screen for Rh antibodies. Subjects and Methods: Data was analyzed using Stata 16.1 software; Categorical data was summarized using frequency and percentages while continuous variables were described using the mean and standard deviation or median and interquartile range. Pearson's Chi-square (or Fisher's exact) test was used to test for association between categorical variables and Rh status. P values of ≤0.05 were assumed to be statistically significant. Results: A total of 700 consenting women, comprising 505 pregnant (72.1%) and 195 non-pregnant (27.9%) women were recruited into this study. The mean age was 30.7 ± 4.9 years. All (100%) participants were Rhc positive while 641 (91.6%) were RhD positive and 59 (8.4%) were RhD negative. All 59 RhD negative subjects tested negative for anti-D. There was no statistically significant difference between proportion of RhD-negative women who had a jaundiced baby and the proportion of RhD-positive women who had a jaundiced baby (15.6% vs. 18.6%, P = 0.540). Conclusions: This study did not identify any Rhc and D alloantibodies in the study population suggesting there is a low risk of alloimmunization and HDFN due to anti-Rhc and D in this population

A Rare Case of Nonsecretory Multiple Myeloma in Lagos, Nigeria: A Case Report and Literature Review

Multiple myeloma (MM) is a plasma cell disorder associated with clonal proliferation of plasma cells. Nonsecretory multiple myeloma (NSMM) is a rare variant of MM and accounts for approximately 1% to 5% of all cases. It is defined as symptomatic myeloma without detectable monoclonal immunoglobulin on serum or urine electrophoresis. This variant usually poses a diagnostic challenge to the clinician. We present a 60-year-old Nigerian man who was investigated extensively for bone pain, weight loss, and anaemia. He was eventually diagnosed as having nonsecretory multiple myeloma based on histology and immunohistochemistry results of bone marrow trephine biopsy. He is currently being managed with bortezomib, doxorubicin, and thalidomide, as well as zoledronic acid. He is also on anticoagulation. He continues to show remarkable clinical improvement. We describe this case report and literature review for better awareness amongst medical practitioners and pathologists