A tumoral mass (local recurrence of renal cell carcinoma) causing massive intraabdominal bleeding after blunt abdominal trauma

AbstractBackgroundSolid organ injury after abdominal trauma is a common condition, however, injury of the local recurrent tumoral masses following abdominal trauma is rare. The injuries and bleeding in recurrent tumors tend to be highly serious since they are more fragile. The bleedings caused by renal cell carcinomas and by the traumatic laceration of their recurrence commonly occur in the retroperitoneum.In this report, we present a 55-year-old female patient who underwent emergency surgery due to intraabdominal bleeding and bleeding was from the recurrence of a renal cell carcinomas.Presentation of caseThe 55-year-old female patient was admitted to the emergency service with intraabdominal bleeding. Physical examination revealed tenderness in the right lower quadrant, particularly in the traumatic area. Ultrasonography and computed tomography revealed diffuse intraabdominal fluid and a ruptured bleeding mass was excised. Pathological analysis indicated that the mass was isolated local recurrence of renal cell carcinoma.DiscussionSolid organ injury caused by blunt abdominal trauma may be accompanied by tumoral laceration; however, minor bleeding may occur in cases with blunt trauma, coexistence of blunt abdominal trauma with local recurrence and massive bleeding is extremely rare.Control of bleeding is more challenging in tumoral tissues compared to normal tissues. The bleeding intraabdominal area rather than the retroperitoneal area, and this condition was attributed to the peritoneal tear caused by the trauma.ConclusionLocal recurrent tumoral masses may be the source of the intraabdominal massive bleeding after blunt trauma

At gübresı̇ ve arıtma çamuru kompostunun, xerofluvent bı̇r toprağın özellı̇klerı̇ne ve bı̇tkı̇sel üretı̇me etkı̇sı̇

Bu çalışmada at gübresi kompostu ve arıtma çamuru kompostu materyallerinin organik gübre olarak hem teksel (2t/da ve 4 t/da), hem birlikte uygulaması yapılmış ve mineral gübre uygulaması altındaki xerofluvent topraklarda mısır bitkisi yetiştirilmiştir. Çalışma kısa süreli olarak iki farklı bolgelde gerçekleştirilmiştir. Uygulamaların toprak verimlilik özellikleri ile mısır bitkisinin mikro element ve mısır verimi parametreleri üzerine (koçan çapı, koçan boyu, tane sayısı, yaprak sayısı) etkileri incelenmiştir. Her bölümde (killi ve kumlu) toprakların , bazı fiziksel ve kimıyasal ozellikleri arıtma çamuru kompostunun teksel ve at gübresi kompostuyla birlikte kullanıldığı uygulamalar ile artış göstermiştir. Killi tın topraklarda, 4 t/da arıtıma çamuru kompostu teksel ve at gübresi kompostuyla birlikte aynı oranda uygulamasının, toprakların organik madde, alınabilir N, P, ve K içeriklerini olumlu yönde etkilenmiş, bu içerikler kontrole göre artış göstermiştir. En yüksek koçan verimi sırasıyla 4 t/da arıtma çamuru teksel ve at gübresi kompostu karışımı ile elde edilirken, toprakların ve bitkilerin mikro element içerikleri arıtma çamuru kompostu uygulamaları ile artış göstermiş fakat her iki ortamda da bitkisel üretime zarar verecek konsantrasyonlara ulaşmamıştır. HA, TK, SN, SSI, DO ve SY gibi toprağın temel fiziksel parametreleri, tüm uygulamalar, özellikle de arıtma çamuru kompostu uygulamalarında (AÇK-4) iyileşmeler göstermiştir. Kumlu topraklarda , 4t/ arıtma çamuru kompostu uygulamasının toprakların TK, SY, DO ve DY iyileştirmiştir. Arıtma çamuru kompostu uygulamalarının teksel ya da at gübresi kompostu ile birlikte gerçekleştirildiği durumlarda toprakların özellikle Kireç , EC ve alınabilir Cu içerikleri kapsamlarında artış sağladığı saptanmıştır. Fe, Zn, Cu ve Mn elementleri AÇK-4 ve NPK uygulanan parsellerdeki yaprak ve gövdelerde daha fazla çıkmıştır. Mısır verimi açısından , en yüksek koçan boyu ve en fazla yaprak sayısı arıtma çamuru kompostu uygulamalarının teksel ya da at gübresi kompostu ile birlikte yapılması ile elde edilmiştir . En yüksek koçan tane sayısı ve en yüksek koçan çapı sadece arıtma çamur kompostu uygulamaları ile elde edilmiştir.In this study the sewage sludge compost and the horse manure compost were applied alone or , in combination with the NPK mineral fertilizer to a xerofluvent soil. The study was carried out shortly in two different regions. Soil fertility characteristics of the applications, corn plant micro and macro elements and heavy metal content, corn yield parameters (cob diameter, cob length, grain number, number of leaves) were investigated. In each section (clay and sandy) soils, some physical and chemical properties have increased with applications where the treatment sludge is used with monopoly and horse manure. In clay loam soils, the application of sewage sludge compost to 4 t / da in combination with monopoly and horse manure in the same proportion showed positive effects on the organic matter, available N, P, and K contents of the soils, these contents increased compared to the control. While the highest yield of cobs was obtained with a mixture of sludge and horse manure compost at 4 t / h respectively, the microelement content of soils and plants increased with purification sludge applications, but it did not reach concentrations that would damage vegetable production in both environments. The basic physical parameters of soil such as HA, TK, SN, SSI, DO and SY showed improvements in all applications, especially in purification sludge compost applications (AÇK-4). In sandy soils, the application of 4t / purification mud compost improved the soils TK, SY, DO and DY. In cases where treatment sludge applications are carried out together with single or horse manure compost, it has been determined that the soils increase especially in the scope of CaCO3 % , EC and obtainable Cu contents. Fe, Zn, Cu and Mn elements were more exposed in the leaves and stems in the plots where AÇK-4 and NPK were applied. In terms of corn yield, the highest crop length and the highest number of leaves were obtained with the monopoly or horse manure compost of the sludge applications. The highest number of stems and the highest diameter of sticks were obtained only by purification sludge compost applications

Differential effects of p38 MAP kinase inhibitors SB203580 and SB202190 on growth and migration of human MDA-MB-231 cancer cell line

WOS: 000405499100014PubMed ID: 28393288p38 mitogen-activated protein kinase (MAPK) belongs to the MAPK superfamily, phosphorylating serine and/or threonine residues of the target proteins. The activation of p38 MAPK leads to cell growth, differentiation, inflammation, survival or apoptosis. In this study, we tested the effect of two highly specific and potent inhibitors of p38 MAPK (namely, SB203580 and SB202190) on human breast cancer cell line MDA-MB-231 to elucidate the controversial role of p38 MAPK on cell proliferation and/or cell migration/metastasis further. It was determined that the IC50 value of SB203580 was 85.1 mu M, while that of SB202190 was 46.6 mu M, suggesting that SB202190 is slightly more effective than SB203580. To verify the effect of each inhibitor on cell proliferation and cytotoxicity, the cells were treated with various doses of SB203580 and SB202190 and examined using iCELLigence system. No significant effect of 1 and 5 mu M of both inhibitors were seen on cell proliferation as compared to the DMSO-treated control cells for up to 96 h. On the other hand, both SB203580 and SB202190 significantly prevented cell proliferation at a concentration of 50 mu M. SB202190 was again more effective than SB203580. Afterwards, we tested the effect of each inhibitor on cell migration using wound assay. Both SB203580 and SB202190 significantly reduced cell migration in a time-dependent manner at a concentration of 50 mu M. However, interestingly it was observed that a low and noncytotoxic dose of 5 mu M of SB203580 and SB202190 also did cause significant cell migration inhibition at 48 h of the treatment, corroborating the fact that p38 MAPK pathway has a critical role in cell migration/metastasis. Then, we tested whether each p38 MAPK inhibitor has any effect on cell adhesion during a treatment period of 3 h using iCELLigence system. A concentration of only 50 mu M of SB202190 reduced cell adhesion for about 1.5 h (p < 0.001); after that period of time, cell adhesion in 50 mu M SB202190-treated cells returned to the level of the control cells. To determine the mechanism of growth and cell migration inhibitory effects of p38 MAPK inhibitors, the activation/inactivation of various proteins and enzymes was subsequently analyzed by PathScan (R) Intracellular Signaling Array kit. The ERK1/2 phosphorylation level was not modified by low concentrations (1 or 5 mu M) of SB202190 and SB203580; while a high concentration (50 mu M) of both inhibitors caused significant reductions in the ERK1/2 phosphorylation. In addition, it was determined that both p38 MAPK inhibitors caused significant increases on the Ser15 phosphorylation of mutant p53 in MDA-MB-231 under these experimental conditions; while SB202190 was more potent than SB203580.Dumlupinar University [2015-85]This study was funded by Dumlupinar University Scientific Project No. 2015-85

Kütahya -Simav Yöresi Sera Topraklarının ve Sulama Suyu Özelliklerinin Araştırılması Üzerine Bir Ön Çalışma

The study was carried out to investigate some quality properties of greenhouse soils and irrigation waters in the Simav region of Kutahya. For this aim, soil and water samples were taken from 20 different greenhouses. Soil samples were taken two times before and after planting period; while water samples were taken three times before, middle and after planting period. Texture, pH, electrical conductivity, organic matter and the concentrations of macro and micro elements and heavy metals in the soil samples were analyzed. Also, the chemical properties of irrigation water samples were investigated. According to the results; the majority of soils were found light textured, slightly acidic, low salinity and they contained low amount of organic matter. the total nitrogen (N) amounts of soil samples were rich level; available phosphorus (P), potassium (K), magnesium (Mg) and copper (Cu) amounts were high level. Extractable sodium (Na) and available calcium (Ca) contents were moderate level; available iron (Fe) and manganese (Mn) were efficient level; zinc amounts were low level before planting period and high level after planting period. Moreover, it was observed that heavy metal concentrations of soils did not exceed limit values. When the analyses of chemical properties of water samples, it was observed that water was suitable for irrigation and classified C2S1 according to U.S. Salinity Laboratory System classification.Bu çalışma Kütahya'nın Simav ilçesindeki seralardan alınan su ve toprak örneklerinin kalite özelliklerinin araştırılması amacıyla yapılmıştır. Bu amaçla Simav yöresindeki 20 farklı seradan toprak ve su örneği alınmıştır. Toprak örnekleri, seralarda başlayan bitkisel üretim öncesi ve sonrası olmak üzere iki kez; su örnekleri ise üretim başlangıcı, ortası ve sonunda olmak üzere üç defa alınmıştır. Toprak örneklerinde bünye, pH, elektriksel iletkenlik, organik madde, makro ve mikro elementler ile ağır metal analizleri yapılmıştır. Su örneklerinin ise kimyasal özellikleri incelenmiştir. Analiz sonuçlarına göre toprak örneklerinin büyük bir kısmının hafif bünyeli, hafif asit, tuzsuz, az düzeyde organik madde içerdiği bulunmuştur. Toprakların toplam azot (N) kapsamı bakımından zengin; alınabilir fosfor (P), potasyum (K), magnezyum (Mg) ve bakır (Cu) bakımından ise fazla düzeyde olduğu gözlenmiştir. Alınabilir sodyum (Na) ve kalsiyum (Ca) içeriği bakımından orta; demir (Fe) ve mangan (Mn) bakımından yeterli; çinko (Zn) bakımından üretim öncesi düşük, üretim sonrası ise yüksek olarak belirlenmiştir. Ayrıca toprakların ağır metal konsantrasyonlarının sınır değerlerini aşmadığı gözlenmiştir. Su örneklerinin kimyasal özelliklerinin incelenmesi sonucunda ise sulama suyu olarak kullanımında herhangi bir sakınca olmadığı ve ABD Tuzluluk Laboratuvarı Sistemi'ne göre sınıflandırıldığında C2S1 sınıfına girerek kullanılabilir nitelikte olduğu görülmüştür