In vitro antibacterial activity of Synclisa scabrida whole root extracts

Ethanolic, cold water and hot water extracts of the root of Synclisia scabrida were tested, by the agarwell diffusion and macro-broth methods, for activity against clinical and typed strains of Escherichia coli, Salmonella sp., Staphylococcus aureus, Pseudomonas aeruginosa, Proteus sp. and Bacillussubtilis. The ethanolic extract was bactericidal at the minimum inhibitory concentration (MIC) against 8 of the 10 test organisms (MIC, 3.125 to 12.50 mg/ml and MBC, 3.125 to 25.00 mg/ml). The cold water extract was bactericidal against E. coli (clin) and B. subtilis ATCC 6051 strains and bacteriostatic against 5 others while the hot water extract was bacteriostatic against 2 of the organisms at MIC. Three column chromatographic fractions of the crude ethanolic extract, Fss-1, Fss-2, and Fss-3, were active against 5 to 8 of the 10 test bacterial strains. Fss-3 was bacteriostatic against the Proteus sp. (clin), which was not susceptible to the crude ethanolic extract. Conversely, the crude ethanolic extract was bactericidal against P. aeruginosa ATCC 10145 to which none of the fractions showed activity. Thus, the results justify the folklore application of extracts of S. scabrida in treatment of bacterialgastroenteritis among other diseases

Treatment of Brewery Waste Water in a Fluidized Bed Digester

This study examined anaerobic fluidized- bed reactor performance employed to treat brewery waste water in terms of treatment efficiency on COD and BOD5 reduction under different hydraulic retention time (HRT). It employed a fluidized-bed digester, with liquid volumetric flow rate of 1.7 x 10-6m3/s, superficial velocity of 0.0012 m/s and 0.00026m/s which achieved the velocity  required to fluidize 20g activated carbon bed of height 0.03m with 0.384 voidage. The pressure drop across the fluidized-bed was calculated to be 1.848 N/m2. The COD and BOD5 concentration reduction efficiencies were monitored .The results obtained indicate that as HRT increases, the concentration of COD and BOD5 initially at 7137.67 COD mg/l and 2177 BOD5 mg/l decreased to final concentration of 1845.92 mg COD/l and 154.17 mg BOD5/l respectively at10h. The COD and BOD5 reduction efficiencies were observed to be 74.1% and 92.9% at the 10h HRT. The experimental data obtained for COD reduction in this work was used for determination of kinetic parameters from modified mixed-flow equation based on Michaelis-Menten’s kinetics and the correlation coefficient was  0.944 .The maximum substrate utilization rate, rmax and the Michaelis-Menten’s constant, KM, were determined to be 301.5 mg COD/l. h and 1345 mg COD/l respectively. Correction factor (F) of 1.1 when applied to the data will fitted simple mixed flow Michaelis Menten kinetic model well. The deviation from typical Michaelis Menten kinetic model can be attributed to acclimatization time required for effective digestion, flow dynamics in the reactor and existence of multi-organism media which would have promoted other side reactions. Organisms identified from the anaerobic digester include clostridium spp, peptococcus anaerobus, bfidobacteriums spp, desulphoribrio spp, corynebacterium spp, lacto. Keywords: Anaerobic decomposition; Brewery wastewater; Fluidized-bed reactor; Modified Michaelis-Menten’s kinetics

Effects of human and porcine bile on the proteome of Helicobacter hepaticus

<p>Abstract</p> <p>Background</p> <p><it>Helicobacter hepaticus </it>colonizes the intestine and liver of mice causing hepatobiliary disorders such as hepatitis and hepatocellular carcinoma, and has also been associated with inflammatory bowel disease in children. In its habitat, <it>H. hepaticus </it>must encounter bile which has potent antibacterial properties. To elucidate virulence and host-specific adaptation mechanisms of <it>H. hepaticus </it>modulated by human or porcine bile, a proteomic study of its response to the two types of bile was performed employing two-dimensional gel electrophoresis (2-DE) and mass spectrometry.</p> <p>Results</p> <p>The 2-DE and mass spectrometry analyses of the proteome revealed that 46 proteins of <it>H. hepaticus </it>were differentially expressed in human bile, 18 up-regulated and 28 down-regulated. In the case of porcine bile, 32 proteins were differentially expressed of which 19 were up-regulated, and 13 were down-regulated. Functional classifications revealed that identified proteins participated in various biological functions including stress response, energy metabolism, membrane stability, motility, virulence and colonization. Selected genes were analyzed by RT-PCR to provide internal validation for the proteomic data as well as provide insight into specific expressions of motility, colonization and virulence genes of <it>H. hepaticus </it>in response to human or porcine bile.</p> <p>Conclusions</p> <p>Overall, the data suggested that bile is an important factor that determines virulence, host adaptation, localization and colonization of specific niches within host environment.</p

Soul Mate Selection Among Adolescents: The Kinetics and Health Perceptions and Interpretations

Soulmate is a brother/sister relationship, practiced and known by many people but just a few especially adolescents understand the exact meaning hence the need to provide adequate information regarding the selection. Adolescents have the tendency to deviate from social norms in search of identity and most often unsure of themselves as most of their experiences rest in the hands of their peer groups. Soulmate comes in form of stranger, healing, wrecking ball, twin and true love soulmates. These are highlighted in the elements of soulmate as one who makes you feel entirely whole, healed, and intact like no piece is missing. Adolescents make selection on the basis of physical attractiveness, economic and social status, age, religion. Finding a true soulmate result in having a feeling of security and protection, falling in love, sharing the same life goals, respect for each other’s differences and opinions. However, wrong mate choices could result in social and health consequences such as prostitution, medical problems and suicide. Kinetics and Health strongly emphasize on aesthetics, general wellness including spiritual wellbeing as strong indices of wellness which could be cached on during mate selection. Conclusively, mate selection is a matter of destiny ordered by God but as two loving people who have settled for each other’s strength or weakness, the decision is yours. The recommendation among others is that adolescents should be counseled to focus selection of mate on attitude as against physical attributes and made a priority among undergraduate students due to growing cases of rape in Nigeria.Key Words: Soulmate, Adolescent, Kinetics and Health DOI: 10.7176/JHMN/79-06 Publication date:August 31st 202

Factors Affecting Biogas Production during Anaerobic Decomposition of Brewery effluent- wastewater in a Fluidized Bed Digester.

This  work determined the dependency of biogas and carbon (iv) oxide production on microbial concentration X (Cfu/l), hydraulic retention time (HRT), ratio of total volatile fatty acid (TVFA) and total alkalinity K, during anaerobic decomposition of brewery effluent wastewater in a fluidized-bed reactor system. The volume of biogas and carbon (iv) oxide produced were monitored as the treatment time progressed. For  brewery waste water volume of 2 litres treated in the bioreactor, the volume of biogas rich in methane, produced in this work was described as s-curve with initial volume of biogas produced as 0.003l  at 4h HRT and maximum volume of 0.0045l at 8 h HRT.Similarly,0.0033l of carbon(iv)oxide was recorded at 2 h HRT and it achieved its maximum of 0.0042l at 8 h HRT. Total microbial count was conducted on the reactor sample water. The maximum concentration of the gases recorded at 8 h HRT corresponded to the favourable operating environment and good system stability ratio  (VFA/Alkalinity)&lt;0.5 achieved. Key words: Anaerobic decomposition, brewery wastewater, fluidized-bed reactor, Biogas, carbon (iv) oxide

Responses of selected inflammatory, kidney and liver function markers in Serum of Nigerian Children with Severe Falciparum Malaria to treatment with artesunate/artemether-lumefantrine combination therapy

Malaria tolerance is a defence strategy that limits the damage caused by Plasmodium species irrespective of pathogen burden. The mechanisms responsible for this, responses of these mechanisms and their impact on organs to treatment have not been extensively studied. Thus, in this study, serum levels of selected pro- and anti-inflammatory markers, liver and kidney function indices with leucocytes indices in 100 children (1-10 years) with severe falciparum malaria were determined before treatment, at 48 hours during treatment and 48 hours after treatment with WHO recommended dosage of artesunate/artemether-lumefantrine combination therapy using standard methods. Data were analysed using SPSS, differences were considered significant at p&lt;0.05. The results revealed that the serum levels of interleukin-12 (IL-12), interleukin-4 (IL-4), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), C-reactive protein (CRP), nitric oxide (NO), creatinine, albumin, total protein and conjugated bilirubin were not significantly changed at higher parasite densities before treatment. Only serum IL-4, CRP, total bilirubin, urea and creatinine levels and alanine aminotransferase activity were significantly reduced below the ranges of those with severe malaria. The results suggest a self-protective feed-back control, indicating tolerance, which reduced the adverse effects of the disease on kidney and liver functions at higher parasite densities. The results also suggest serum IL-12, IL-4, TNF-α, IFN-γ, CRP and NO levels as immune-protective markers for tolerance and serum IL-4 level as an effective marker for disease severity and recovery from the disease in children with severe malariaKeywords: Immunity, falciparum malaria, inflammatory markers, childrenAfr. J. Biomed. Res. Vol. 22 (May, 2019); 165-17

The Mobile Conference Information System: Unwiring Academic Conferences with Wireless Mobile Computing

Wireless mobile computing promises to usher in the next major paradigm in personal computing. Handheld computers in particular are truly portable and they are becoming increasingly capable of meeting most users\u27 computing needs. In this paper we outline a vision for deploying wireless mobile computing technology within the realm of professional conferences by creating a Mobile Conference Information System (MCIS). With detailed descriptions of applications and hypothetical usage scenarios, we describe how the system can be used to access conference information, to network people, and enhance common conference services. We also describe several constraints, limitations, and challenges with this concept, and we suggest how these problems can be overcome. In this paper we consolidate many current applications of wireless networked computing into one comprehensive system; we highlight several of the complexities and challenges that apply to any wirelessly mobile information system; and we offer a vision for a valuable platform for future research on the use of mobile technologies. The MCIS will significantly change how conferences are managed and enhance the experiences of the attendees

Comparative efficacy of three plant extracts for the control of leaf spot disease in fluted pumpkin (Telfairia occidentalis Hook F.)

The production and leaf quality of Fluted pumpkin (Telfairia occidentalis hook f) in Nigeria are threatened by leaf spot and blight. The comparative efficacy of Siam weed (Chromolaena odorata (L.), Utazi leaf (Gongronema latifolium and Bitter leaf (Vernonia amygdalina) and a synthetic fungicide (Forcelet) against the mycelia growth of the leaf spot pathogen (Phoma sorghina), leaf spot disease, growth and yield of fluted pumpkin (Telfairia occidentalis hook f) was studied in vitro and in vivo. In 2013 and 2014. The experiments design for the in vitro experiment was Completely Randomize Design (CRD) with six replicates and Randomize Complete Block Design (RCBD) with four replications in the in vitro study. All plant extracts consistently inhibited the growth of P. sorghina in vitro, and suppressed leaf spot development by between 26.2 and 53.54% in 2013 and by between 26.3 and 51.79% in 2014 under field conditions. Leaf and pod yield were also enhanced, C. odorata leaf extract was significantly (p≤0.05) superior to all other treatments followed by V. amygdalina whose effect did not differ significantly (P≥ 0.05) from Forcelet in all the parameters measured. These results show that leaf extracts of C.odorata and V. amygdalina could be used for the control of leaf spot disease and enhanced yield in fluted pumpkin. Keyword: Efficacy, Plant extract, Control, Leaf spot disease, Yield

Anti-inflammatory activity of fatty extract of Vitalleria paradoxa Kernel (Shea butter) and pattern of its clinical use in arthritis in Enugu, South East Nigeria

Background: The fatty seed extract of Vitellaria paradoxa Kernel (Sapotaceae) commonly called “Shea butter”, is a popular remedy for arthritis in west Africa. This study investigated the knowledge, prevalence and pattern of use of Shea butter in clinically diagnosed arthritic patients as well as its effects on acute (topical and systemic) and chronic inflammation in rodents.Methods: Knowledge, prevalence and pattern of use were determined using pre-tested questionnaire in clinically diagnosed patients whereas the anti-inflammatory activity was studied using xylene-induced mouse ear edema, carrageenan-induced rat paw edema, formaldehyde-induced arthritis in rats and cotton pellet granuloma test in rats.Results: The results showed that of the 164 respondents, 94.1% know about Shea butter and 59.6% have used it mainly as a massage ointment once or twice daily. However, 73.7% of the users combine this remedy with analgesics to achieve relief. The pharmacological tests showed that topical application of Shea butter inhibited acute edema of the mouse ear. Systemic oral administration caused significant (p<0.05) suppression of the development of systemic acute edema of the rat paw in a non-dose related manner. Twice daily topical application of Shea butter significantly (p<0.05) inhibited the edematous response to formaldehyde arthritis whereas once daily administration was not effective. Shea butter also caused a significant (p<0.05) non-dose related inhibition of granuloma tissue formation on implanted cotton pellets.Conclusions: These findings provide a scientific rationale for the use of Shea butter in treatment of disorders of inflammation in traditional medicine

Demand Response for Caborhydrate and Food Insecurity Dynamics in Imo State, Nigeria

Demand for cabohydrate food stuffs responds its accesibility and unsteady price regime, thus food insecurity dynamics in Imo State. The study investigated a cheap and steady market demand for carbohydate foodstuffs in the state using 50 weekly observations on mean own prices, quantities demanded of both gari and rice as substitute from the management of Owerri Market Traders Assocition (OMATA) between January to December 2012. Data were analysed using simple decriptive statistics, charts and econometric tools. In general terms gari had a normal demand behaviour between June and August but abnonormal during festival periods. All the Error correction vectors -0.0545, -0.444 and -0.425 for Ekeonunwa, Relief and Ihiagwa demand models respectively were statistically significant ( ). Therefore, the speed of adjustment of lagged prices to long-run actual demand were completed in the next period, hence food insecurity flunctuates. Lagged own price of gari is negative and inelastic in all the models except that of Ihiagwa market (1.277). Gari is thus a normal goods in all the markets but has a more than 0.28% proportionate increase in demand with a unit decrease in its own price in Ihiagwa market. Therefore, food security will be achieved if there is a more effort to reduce the price of gari in Ihiagwa market. Rice is better subtitute to gari in Relief than the rest of the markets, hence increase in supply of rice in Relief market is imperative for food security in the area. The study therefore, recommends a steady reduced price conditions in both Relief and Ihiagwa markets for rice and gari respectively to ensure increased returns and supply for sellers and steady food security in the state. Keywords: Demand, Response, Food Security, Carbohydrate, Markets and Error Correction Vector